ABSTRACT
Objective:
To establish a
high-performance liquid chromatography-
tandem mass spectrometry (
LC-MS/MS)
method for the
determination of voliconazole (VRC), posaconazole (PCZ), and linazolam (LNZ) in
human serum.
Methods:
This study is a methodological validation by
LC-MS/MS. The
blood concentration results of VRC, PCZ, and LNZ in our
hospital′s anti-
infection patients were collected.
Voriconazole, Posaconazole, and
Linezolid were accurately weighed and prepared.
Linezolid-[2H3] was used as the internal standard. After gradient elution on the ACE PFP column, the residuals were analyzed by
LC-MS/MS in the positive electrospray ionization mode and multiple reaction monitor (MRM) mode. The
method′s linearity, precision, lower
limit of detection, and recovery rate were validated according to standard guidelines.
Results:
The linear correlation coefficient ( r) of the standard curve was above 0.99 ( r>0.99). The linear range of VRC and PCZ were 0.10 mg/L~10.00 mg/L, and the lower
limit of detection were 0.01 mg/L. The linear range of LNZ was 0.50 mg/L~50.00 mg/L, and the lower
limit of detection was 0.05 mg/L. The recoveries of VRC, PCZ and LNZ were 90.96%-103.18%, 91.84%-99.17%, and 97.04%-100.41%, respectively. Intra-and inter-batch precision (% CV) for VRC were less than 8.30%. Intra-and inter-batch precision (% CV) for PCZ was less than 9.78%. Intra-and inter-batch precision (% CV) for LNZ was less than 7.14%.
Drug concentrations in 155 cases of VRC, 44 cases of PCZ, and 59 cases of LNZ were detected.
Conclusion:
We have established an
LC-MS/MS
method for the rapid, accurate, highly specific
determination of VRC, PCZ, and LNZ concentrations in
human serum. This
method is suitable for analyzing large clinical sample sets.