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Hepatology International ; 17(Supplement 1):S265-S266, 2023.
Artigo em Inglês | EMBASE | ID: covidwho-2327204


Background: Hepatocellular carcinoma (HCC) is the second leading cause of malignancy-related mortality and the fifth most common worldwide. Immuno-cancer microenvironment (ICME) was highlighted recently because scientists want to unlock the detailed mechanism in carcinogenesis pathway and find the novel interactions in ICME. Besides, single cell analysis could mitigate the interrupted signals between cells and tissues. On the other hand, COVID-19 angiotensin I converting enzyme (ACE) previously was reported associated with cancer. However, the robust association between COVID-19 and HCC ICME is still unaddressed. Aim(s): We plan to investigate the COVID-19 ACE relevant genes to HCC ICME regarding survival. Method(s): We used Reactome for COVID-19 ACE gene pathway mapping and explored the positive relevant gene expression. DISCO website was applied for single cell analyses using the above-collected genes from Reactome. Finally, we implanted the biomedical informatics into TIMER 2.0 for ICME survival analyses. Result(s): In Fig. 1, the gene-gene interaction mapping was shown. We collected 13 genes (CPB2, ACE2, AGT, MME, ANPEP, CPA3, ENPEP, GZMH, CTSZ, CTSD, CES1, ATP6AP2, and AOPEP) for further single cell relevant analyses, in Table 1, with detailed expression level (TPM). Among the above 13 genes, AGT, GZMH, CTSZ, CTSD, CES1, and ATP6AP2 were strongly expressed in liver tissue. We then applied the initial 13 genes to TIMER 2.0 for HCC ICME 2-year survival analyses. CPA3 and GZMH low expressions with high macrophage infiltration in HCC ICME showed significantly worse 2-year cumulative survival [hazard ratio (HR):CPA3 2.21, p-value 0.018;GZMH 2.07, p-value 0.0341]. ACE2, CPB2, AGT, MME, ANPEP, ENPEP, CTSZ, CTSD, CES1, and ATP6AP2 high expressions with high macrophage infiltration in HCC ICME revealed significantly worse 2-year cumulative survival. Conclusion(s): We demonstrate that ACE2 was strongly associated with HCC clinical survival with macrophage infiltration. However, the bidirectional translational roles about ACE2 relevant genes in HCC should be documented.

New Journal of Chemistry ; : 7, 2022.
Artigo em Inglês | Web of Science | ID: covidwho-1704237


Carbohydrate microarrays, featuring high-throughput and rapid detection, provide a powerful tool for getting better insights into carbohydrate-mediated interactions. In this study, poly(2-methylacrylic acid) (pMAA)-modified substrates have been developed for fabricating carbohydrate microarrays. The abundant carboxyl groups of the pMAA polymer provide large amounts of reaction sites for subsequent carbohydrate immobilization. After the activation of the carboxyl groups, amino-modified carbohydrates were covalently and site-specifically immobilized on the pMAA-based substrates. As a typical example, the specific interaction between 4-aminophenyl alpha-d-mannopyranoside (alpha-Man) and fluorescein isothiocyanate labelled concanavalin A (ConA-FITC) was investigated using a pMAA-based carbohydrate microarray. It exhibited a low limit of detection of both spotted alpha-Man (10 mu M) and ConA-FITC in solution (9.26 nM) without any further signal amplification. The practical application of the pMAA-based microarrays was validated by investigating the specific interactions of polysaccharides and SARS-CoV-2 spike protein, and fabricating microarrays containing other biomolecules (e.g., glycoprotein and DNA). The proposed pMAA-based microarrays provide a promising tool in bioanalytical and biomedical studies.

World Chinese Journal of Digestology ; 28(14):563-569, 2020.
Artigo em Chinês | Scopus | ID: covidwho-946264


Coronavirus disease-2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has evolved into a global pandemic and brought great crises and disasters to mankind. The disease is transmitted mainly through respiratory droplets and close contact, but whether it can be transmitted through the digestive tract and by faeces has been a problem of great concern to the medical community and the general public. This review discusses and analyzes the problem in terms of clinical manifestations and digestive system symptoms of COVID-19, detection and isolation of virus, expression and distribution of angiotensin-converting enzyme 2 receptor, etc. There is a potential risk of infection and transmission of SARS-CoV-2 through the digestive tract. It is suggested to carry out further confirmatory research and strengthen effective means of prevention and control. © The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved.