Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 114.767
Filtrar
Adicionar filtros

Tipo de documento
Ano de publicação
Intervalo de ano
2.
Biomedica ; 41(Sp. 2): 86-102, 2021 10 15.
Artigo em Inglês, Espanhol | MEDLINE | ID: covidwho-1529016

RESUMO

INTRODUCTION: Immunological markers have been described during COVID-19 and persist after recovery. These immune markers are associated with clinical features among SARSCoV-2 infected individuals. Nevertheless, studies reporting a comprehensive analysis of the immune changes occurring during SARS-CoV-2 infection are still limited. OBJECTIVE: To evaluate the production of proinflammatory cytokines, the antibody response, and the phenotype and function of NK cells and T cells in a Colombian family cluster with SARS-CoV-2 infection. MATERIALS AND METHODS: Proinflammatory cytokines were evaluated by RT-PCR and ELISA. The frequency, phenotype, and function of NK cells (cocultures with K562 cells) and T-cells (stimulated with spike/RdRp peptides) were assessed by flow cytometry. Anti-SARS-CoV-2 antibodies were determined using indirect immunofluorescence and plaque reduction neutralization assay. RESULTS: During COVID-19, we observed a high proinflammatory-cytokine production and a reduced CD56bright-NK cell and cytotoxic response. Compared with healthy controls, infected individuals had a higher frequency of dysfunctional CD8+ T cells CD38+HLA-DR-. During the acute phase, CD8+ T cells stimulated with viral peptides exhibited a monofunctional response characterized by high IL-10 production. However, during recovery, we observed a bifunctional response characterized by the co-expression of CD107a and granzyme B or perforin. CONCLUSION: Although the proinflammatory response is a hallmark of SARS-CoV-2 infection, other phenotypic and functional alterations in NK cells and CD8+ T cells could be associated with the outcome of COVID-19. However, additional studies are required to understand these alterations and to guide future immunotherapy strategies.


Assuntos
COVID-19/imunologia , Células Matadoras Naturais , SARS-CoV-2/imunologia , Linfócitos T , Adulto , Anticorpos Antivirais/análise , Antígeno CD56/imunologia , Estudos de Casos e Controles , Colômbia , Saúde da Família , Granzimas/metabolismo , Humanos , Interleucina-10/metabolismo , Interleucina-1beta/sangue , Interleucina-6/sangue , Interleucina-8/sangue , Células K562 , Células Matadoras Naturais/citologia , Células Matadoras Naturais/imunologia , Ativação Linfocitária , Masculino , Pessoa de Meia-Idade , Perforina/metabolismo , Fenótipo , Receptores CCR7/metabolismo , Linfócitos T/citologia , Linfócitos T/imunologia , Fator de Necrose Tumoral alfa/sangue , Adulto Jovem
3.
Biosci Trends ; 15(5): 345-349, 2021 Nov 21.
Artigo em Inglês | MEDLINE | ID: covidwho-1528987

RESUMO

Coronavirus disease 19 (COVID-19) continues to rage as a global pandemic. A number of potential therapeutic agents have been explored over the past year or two. However, numerous drugs that were expected to prove highly effective, such as lopinavir/ritonavir and remdesivir, have been found to have little benefit in large clinical trials. Interleukin-6 receptor antagonists, glucocorticoids, Janus kinase inhibitors, and some antivirals have been found to provide significant benefits in terms of reducing viral load, reducing the time of nucleic acid conversion, or improving survival. For example, bamlanivimab and etesevimab, which are newly designed monoclonal antibodies against the surface spike protein S1 subunit receptor-binding domain (RBD) of SARS-CoV-2, have a significant effect on reducing the viral load and the hospitalization rate of patients with mild COVID-19. Several vaccines against SARS-CoV-2 have been widely administered worldwide and have provided good protection. Nevertheless, the increasingly hardy variants of the virus have raised the requirements for vaccine design. Perhaps RBD-based vaccines are a viable way to defend against variants, but this still needs to be verified in a large sample. Therefore, this paper provides an update on the treatment options for COVID-19 based on three previously proposed dimensions of drug screening: standard assays of existing broad-spectrum antivirals, screening of chemical libraries, and redevelopment of new, specific drugs.


Assuntos
Antivirais/uso terapêutico , COVID-19/tratamento farmacológico , Reposicionamento de Medicamentos , Animais , Anticorpos Monoclonais/uso terapêutico , Vacinas contra COVID-19 , Humanos , Ensaios Clínicos Controlados Aleatórios como Assunto
4.
JSLS ; 25(4)2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528971

RESUMO

BACKGROUND AND OBJECTIVES: The COVID-19 pandemic dramatically impacted gynecologic surgery. In March 2020, the American College of Surgeons recommended delay of all nonessential invasive procedures. This study characterizes the number and types of procedures performed during the peak pandemic. METHODS: A retrospective cohort study was performed. All patients undergoing gynecological surgery at a large academic hospital system from March 16, 2019 to July 31, 2019 and from March 16, 2020 to July 31, 2020 were evaluated. Data was stratified by three time periods corresponding to state and hospital policy changes. During period 1, no nonessential procedures were advised. During period 2, urgent procedures resumed. During period 3, full surgical reopening was achieved. RESULTS: In 2019, 1,545 gynecologic cases were performed compared with 942 cases in 2020 (39.0% decrease). There was a 73.6% decrease in cases over period 1, a 20.1% decrease over period 2, and a 2.9% increase over period 3. Cases performed by gynecologic oncologists in 2020 accounted for 58.1% of all gynecologic cases over period 1, 29.4% of cases over period 2, and 33.3% of cases over period 3. In 2020, hysterectomy was the most commonly performed procedure, while surgery for endometriosis and uterine fibroids had the greatest decrease in volume. Among emergency procedures, more surgery for ectopic pregnancy was performed in 2020 compared with 2019. CONCLUSION: Many patients had significant delays in receiving gynecologic surgical care during the peak pandemic period. Further studies are indicated to determine the impact of delayed care on patients' quality of life and disease process.


Assuntos
COVID-19 , Pandemias , Feminino , Procedimentos Cirúrgicos em Ginecologia , Hospitais , Humanos , Gravidez , Qualidade de Vida , Estudos Retrospectivos , SARS-CoV-2
5.
J Indian Soc Pedod Prev Dent ; 39(3): 316-320, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528960

RESUMO

Background: Restrictions on routine dental procedures involving aerosols during COVID-19 have resulted in a significant increase in the suffering of pediatric patients. Aim: The study reported the alternative measures followed in our department using SDF during the COVID era when routine elective dental procedures were prohibited. Materials and Methods: The retrospective data of patients aged 2-13 years with carious molar teeth who were treated with silver diamine fluoride without (Group 1) or with (Group 2) caries excavation were collected. Results: One thousand and seventy-two patients (646 males and 426 females) with 2459 carious molar were treated. On follow-up assessment at 3 months, 28 teeth in Group 1 and 21 teeth in Group 2 showed progression of carious lesion using International Caries Detection and Assessment System (ICDAS II criteria). Out of these failure cases, 32 (1.8%) teeth were primary and 17 (2.5%) were permanent. Complete relief in sensitivity/pain on stimulation was reported in 2381 teeth (96.83%), whereas in 78 (3.17%) teeth, mild sensitivity/pain on stimulation was reported. The average time consumed during treatment per tooth in Group 1 was 5.04 min and in Group 2 was 5.78 min. Conclusion: SDF application can be carried out as a nonaerosol-generating procedure and is a simple technique for children and clinicians.


Assuntos
COVID-19 , Cárie Dentária , Aerossóis , Cariostáticos , Criança , Cárie Dentária/tratamento farmacológico , Odontologia , Feminino , Fluoretos Tópicos , Humanos , Masculino , Compostos de Amônio Quaternário , Estudos Retrospectivos , SARS-CoV-2 , Compostos de Prata
6.
Indian J Dent Res ; 32(2): 206-210, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528958

RESUMO

Context: The Corona Virus Disease 2019 (COVID-19) is a contagious disease caused by the novel Coronavirus (2019-nCoV) and was declared a pandemic disease by the World Health Organization (WHO) in March 2020. The nasopharyngeal and the oropharyngeal swabs are being taken during the screening procedure. However, the virus is also present in the oral bio-fluid and hence it could be a potential tool for screening COVID-19 cases. Aim: The aim of the present study was to test the accuracy of whole saliva as a diagnostic specimen in COVID-19. Settings and Design: This cross-sectional, analytical study was conducted on out-patients visiting the COVID-19 hospital. Methods and Material: The whole saliva and the nasopharyngeal/oropharyngeal samples from 309 COVID-19 suspected patients were collected and subjected to RT-PCR analysis. Statistical Analysis Used: The paired t test was used to compare the measured variables (CT values) between the saliva and the swab samples. The positive predictive value (PPV), negative predictive value (NPV), the sensitivity, and the specificity of the tests were calculated for the saliva sample. Results: The saliva and swab results revealed a similar result (ties) in 86.73% of the samples. The sensitivity and the specificity between the swab and saliva samples were 40% and 96.85%, respectively. The positive predictive value of the saliva sample was 73.3%. Conclusions: The sensitivity of whole saliva when compared to the swab samples is low. Large sample studies are needed to validate the role of saliva as a diagnostic tool in COVID-19.


Assuntos
COVID-19 , SARS-CoV-2 , Estudos Transversais , Humanos , Nasofaringe , Pandemias , Saliva
7.
Tidsskr Nor Laegeforen ; 141(2021-14)2021 10 12.
Artigo em Norueguês | MEDLINE | ID: covidwho-1528951
8.
Tidsskr Nor Laegeforen ; 141(2021-14)2021 10 12.
Artigo em Norueguês | MEDLINE | ID: covidwho-1528949

RESUMO

Multisystem inflammatory syndrome is a rare immune-mediated complication of infection with SARS-CoV-2 in children and adolescents. The patients can rapidly become seriously ill with high fever, gastrointestinal symptoms and cardiogenic shock. The goal of treatment is to ensure adequate circulation and prevent late complications by providing anti-inflammatory therapy.


Assuntos
COVID-19 , SARS-CoV-2 , Adolescente , COVID-19/complicações , Criança , Humanos , Síndrome , Síndrome de Resposta Inflamatória Sistêmica
9.
Front Public Health ; 9: 779720, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528877

RESUMO

Introduction: With the approval of COVID-19 vaccinations for children and adolescents in China, parental vaccine hesitancy will emerge as a new challenge with regard to the administration of these vaccines. However, little is known regarding this hesitancy as well as regional differences that may exist between parents from Shandong vs. Zhejiang. Methods: To assess these issues, an online survey was conducted via a Wenjuanxing platform over the period from July 22 to August 14, 2021. Parents from Shandong and Zhejiang were recruited from Wechat groups and results from a total of 917 subjects were analyzed. Factors evaluated in this survey included socio-demographic variables, parental vaccine hesitancy, Parental Attitudes toward Childhood Vaccines (PACV) domains (behavior, safety and efficacy, general attitudes) and social support. Results: Compared with those from Shandong (N = 443), parents from Zhejiang (N = 474) showed significantly higher prevalence rates of COVID-19 vaccine hesitancy (19.4 vs. 11.7%, p = 0.001). Multivariate logistic regression showed that yearly household incomes of ≥120,000 RMB (p = 0.041), medical workers (p = 0.022) and general attitudes of PACV (p = 0.004) were risk factors for vaccine hesitancy among parents from Shandong, while behavior (p = 0.004), safety and efficacy (p < 0.001) and general attitudes of PACV (p = 0.002) were risk factors for parents from Zhejiang. Among parents with vaccine hesitancy (N = 144), concerns over side effects (91.0%) and unknown effects (84.0%) of the COVID-19 vaccine were the most prevalent reasons for hesitancy. Evidence providing proof of vaccine safety (67.4%) and assurance of a low risk of being infected by COVID-19 (60.4%) were the two most effective persuasive factors. Conclusion: Parents from Zhejiang showed a higher prevalence of COVID-19 vaccine hesitancy as compared with those from Shandong. Behavior, safety and efficacy, and general attitudes of PACV were the risk factors associated with this hesitancy in these parents from Zhejiang. Given the identification of the various reasons for parental vaccine hesitancy, different strategies as well as regional adjustments in these strategies will be required for an effective and convincing protocol for childhood vaccinations.


Assuntos
COVID-19 , Vacinas , Adolescente , Vacinas contra COVID-19 , Criança , Estudos Transversais , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Pais , Aceitação pelo Paciente de Cuidados de Saúde , SARS-CoV-2 , Vacinação , Vacinas/efeitos adversos
10.
Front Public Health ; 9: 779501, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528876

RESUMO

This paper examines the effects of stringency measures (provided by the Oxford Coronavirus Government Response Tracker) and total time spent away from home (provided by the Google COVID-19 Community Mobility Reports) on the COVID-19 outcomes (measured by total COVID-19 cases and total deaths related to the COVID-19) in the United States. The paper focuses on the daily data from March 11, 2020 to August 13, 2021. The ordinary least squares and the machine learning estimators show that stringency measures are negatively related to the COVID-19 outcomes. A higher time spent away from home is positively associated with the COVID-19 outcomes. The paper also discusses the potential economic implications for the United States.


Assuntos
COVID-19 , Governo , Humanos , SARS-CoV-2 , Mobilidade Social , Estados Unidos
11.
Front Public Health ; 9: 772782, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528875

RESUMO

Background: Knowledge on the settings and activities associated with a higher risk of SARS-CoV-2 transmission is essential to inform decision-making. We thus designed a case-control study to identify relevant settings for community transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Portugal. Methods: We evaluated 1,088 cases, identified through the national surveillance system, and 787 community controls, recruited using random digit dialing. Sociodemographic characteristics, individual protective measures, and activities or visited settings were obtained through telephone interview. We report sex-, age-, education-, and citizenship-adjusted odds ratios (aOR) with 95% confidence intervals (95% CI). Results: Household overcrowding (aOR = 1.47; 95% CI 1.14-1.91) and work in senior care (4.99; 1.30-33.08) increased while working remotely decreased the risk of infection (0.30; 0.22-0.42). Going to restaurants/other dining spaces (0.73; 0.59-0.91), grocery stores (0.44; 0.34-0.57) or hair salons (0.51; 0.39-0.66), or the use of public transportation did not present a higher risk of infection (0.98; 0.75-1.29), under existing mitigation strategies. Lower education ( ≤ 4 years vs. tertiary education: 1.79; 1.33-2.42) and no Portuguese citizenship (5.47; 3.43-9.22) were important risk factors. Conclusions: The utilization of public transportation, restaurants, and commercial spaces was not associated with increased risk of infection, under capacity restrictions, physical distancing, use of masks, and hygiene measures. Overcrowding, foreign citizenship, low education and working on-site were positively associated with SARS-CoV-2 infection.


Assuntos
COVID-19 , SARS-CoV-2 , Estudos de Casos e Controles , Humanos , Máscaras , Fatores de Risco
12.
Front Public Health ; 9: 755163, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528874

RESUMO

The cognitive attitudes and behaviors of medical post-graduates may be influenced by the coronavirus disease 2019 (COVID-19) pandemic. A cross-sectional study was used from a questionnaire survey in hospitals affiliated with the Zhejiang University School of Medicine. Questionnaire was distributed online including demographic information, cognitive attitudes, and personal protective behaviors. Moreover, personal protective behaviors such as wearing protective equipment were compared between different academic major and gender, respectively. A total of 176 valid questionnaires were obtained. Of the medical post-graduates in this study, (1) 89.67% believed that the COVID-19 pandemic had an impact on their clinical internships, and 40.34% expressed concerns about their infection on inadequate personal protection; (2) 91.48% took personal protection in hospital and 86.36% enhanced personal hygiene; (3) There were no statistically differences in the personal protection by academic major and gender (p > 0.05). This study suggests that the COVID-19 pandemic had an impact on the medical post-graduates' clinical practice, and affected their cognitive attitudes and behaviors. As such, universities and hospitals should increase pandemic prevention training and investment, provide more psychological counseling to their medical post-graduates to reduce their psychological burden, and take measures to reduce the influence of the COVID-19 pandemic on their medical post-graduates' clinical practice.


Assuntos
COVID-19 , Pandemias , China/epidemiologia , Cognição , Estudos Transversais , Conhecimentos, Atitudes e Prática em Saúde , Humanos , Pandemias/prevenção & controle , SARS-CoV-2
13.
Front Public Health ; 9: 747894, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528873

RESUMO

Eighteen months into the COVID-19 pandemic, and as the world struggles with global vaccine equity, emerging variants, and the reality that eradication is years away at soonest, we add to notion of "layered defenses" proposing a conceptual model for better understanding the differential applicability and effectiveness of precautions against SARS-CoV-2 transmission. The prevailing adaptation of Reason's Swiss cheese model conceives of all defensive layers as equally protective, when in reality some are more effective than others. Adapting the hierarchy of controls framework from occupational safety provides a better framework for understanding the relative benefit of different hazard control strategies to minimize the spread of SARS-CoV-2.


Assuntos
COVID-19 , Saúde do Trabalhador , Humanos , Pandemias/prevenção & controle , SARS-CoV-2 , Gestão da Segurança
14.
Front Public Health ; 9: 744706, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528872

RESUMO

The special vulnerability of people with disability (PWD) in the COIVD-19 pandemic has been confirmed by many studies, but data shows that the infection rate of PWD in China is lower than for non-disabled people. We believe that this difference can be attributed to the Chinese government's targeted safeguards for the disabled community during the pandemic, including support for disability empowerment, the establishment of a remote interactive decision-making system, fair vaccine distribution and economic protection for PWD. The professionalism of decision-makers and the maintenance of channels of interaction with PWD are also important. All of these changes have benefitted China's public health policy and legal framework. This system, which has six components (governance, prevention, response, knowledge, coordination, and people), enables the country to quickly formulate a series of safeguards for PWD that have a sufficient legal basis. We believe that China's rapidly improving public health policy and legal framework will make a hugely significant impact by alleviating the impact of the COVID-19 pandemic on the PWD community. Countries should pay more attention to discovering the special needs and obstacles of PWD in the COIVD-19 pandemic: in referring to China's experience, they should explore the protection framework for persons with disabilities and adjust it to their own needs on the basis of national conditions.


Assuntos
COVID-19 , Pessoas com Deficiência , China/epidemiologia , Humanos , Pandemias/prevenção & controle , SARS-CoV-2
15.
Front Public Health ; 9: 736976, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528871

RESUMO

Introduction: Italy was one of the earliest countries to experience a severe COVID-19 epidemic and vaccinating the elderly, who constitute 23% of the population and have experienced the highest mortality rates, is a top priority. Estimating prevalences and understanding risk factors for COVID-19 vaccine hesitancy or refusal are important for development of targeted interventions. Methods: We used data from a specially developed COVID-19 module of PASSI D'Argento, an ongoing surveillance system of residents 65+ years of age to measure the prevalence and identify risk factors for hesitancy and refusal to receive the COVID-19 vaccine. We calculated multinomial regression relative risk ratios to examine the association between demographic characteristics, health status, COVID-19 attitudes and experiences and likely vaccine hesitancy and refusal. Results: Of the 1876 respondents, 55% reported they would accept vaccination and 16% would likely refuse; the remaining 29% were categorized as hesitant. Compared with the acceptance group, we identified several risk factors in common between the hesitancy group and the refusal group, including not having received vaccination against influenza during the previous flu season (hesitancy: RRR = 2.0; 95% CI 1.4-2.9; refusal: RRR = 12.1; 95%CI 7.6-19.4) and lower risk of having had a death from COVID-19 among family or friends (hesitancy: RRR = 4.8; 95%CI 2.0-11.4; refusal: RRR = 15.4; 95%CI 3.7-64.5). The hesitancy group was significantly more likely being worried and they did not know if consequences of the disease would be serious for them. Conclusion: Our findings show the importance of establishing and maintaining active contact between the preventive services, primary care providers and the population because trust is difficult to establish during an emergency like the COVID-19 pandemic. Italian public health is based on a capillary network of general practitioners and having them reach out to their patients who have not previously received influenza vaccine may be a useful strategy for targeting efforts to further encourage uptake of COVID-19 vaccination.


Assuntos
COVID-19 , Clínicos Gerais , Vacinas contra Influenza , Idoso , Vacinas contra COVID-19 , Estudos Transversais , Humanos , Itália/epidemiologia , Pandemias , SARS-CoV-2 , Vacinação
16.
Front Public Health ; 9: 731981, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528870

RESUMO

To date, most of the evidence suggests that smoking is negatively associated with testing positive for SARS-CoV-2. However, evidence has several methodological limitations. Using an outpatient sample population, we analyzed the association of testing positive for SARS-CoV-2 and smoking considering comorbidities, socioeconomic and demographic factors. Baseline data were obtained from a cohort during the first wave of the pandemic in Geneva, Switzerland (March-April 2020). RT-PCR tests were carried out on individuals suspected of having SARS-CoV-2 according to the testing strategy at that time. Logistic regressions were performed to test the association of smoking and testing positive for SARS-CoV-2 and further adjusted for comorbidities, socioeconomic and demographic factors. The sample included 5,169 participants; 60% were women and the mean age was 41 years. The unadjusted OR for testing positive for SARS-CoV-2 was 0.46 (CI: 0.38-0.54). After adjustment for comorbidities, socioeconomic and demographic factors, smoking was still negatively associated with testing positive for SARS-CoV-2 (OR: 0.44; CI: 0.35-0.77). Women (OR: 0.79; CI: 0.69-0.91), higher postal income (OR: 0.97; CI: 0.95-0.99), having respiratory (OR: 0.68; CI: 0.55-0.84) and immunosuppressive disorders (OR: 0.63; CI: 0.44-0.88) also showed independent negative associations with a positive test for SARS-CoV-2. Smoking was negatively associated with a positive test for SARS-CoV-2 independently of comorbidities, socioeconomic and demographic factors. Since having respiratory or immunosuppressive conditions and being females and healthcare workers were similarly negatively associated with SARS-CoV-2 positive testing, we hypothesize that risk factor-related protective or testing behaviors could have induced a negative association with SARS-CoV-2.


Assuntos
COVID-19 , SARS-CoV-2 , Adulto , Feminino , Humanos , Pacientes Ambulatoriais , Fumar/efeitos adversos , Suíça/epidemiologia
17.
Front Public Health ; 9: 679041, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528869

RESUMO

Background: The previous and current studies highlight the psychological distress caused by coronavirus disease 2019 (COVID-19)-associated restrictions among the general population, especially among children and adolescents; however, few studies have examined children and adolescents with a mental disorder. The current study aimed to explore whether youth with mental disorders show a higher pandemic-associated psychological burden than healthy children and adolescents and to determine which psychiatric diagnoses are particularly associated with a higher distress level. Methods: In this study, 144 children and adolescents between the ages of 6 and 18 years with a mental disorder and 48 children and adolescents within the same age range without a mental disorder, and their caregivers, completed questionnaires assessing the pandemic-associated trauma symptoms (the Child Report of Post-Traumatic Symptoms [CROPS] and the Parents Report of Post-Traumatic Symptoms [PROPS]). Additionally, we asked specific questions about the pandemic-associated stress factors, such as financial problems, prolonged screen times, or loneliness. Results: Children and adolescents with a mental illness showed a significantly higher psychological burden than their mentally healthy peers. Female gender was a risk factor for a higher self-reported psychological burden, and younger age was associated with a more extensive parent-reported psychological burden. The patients with a depressive disorder showed significantly higher levels of psychological distress associated with the COVID-19 pandemic than the patients with an attention deficit and/or a conduct disorder. Conclusions: Children and adolescents with a mental illness, particularly, female children and individuals with a depressive disorder, are at an increased risk of suffering from pandemic-associated psychological distress. Adequate mental health care options, such as telepsychiatry, are indispensable.


Assuntos
Transtorno do Deficit de Atenção com Hiperatividade , COVID-19 , Psiquiatria , Telemedicina , Adolescente , Criança , Feminino , Humanos , Pandemias , SARS-CoV-2
18.
Front Cell Infect Microbiol ; 11: 717068, 2021.
Artigo em Inglês | MEDLINE | ID: covidwho-1528814

RESUMO

This study aimed to detect the SARS-COV2 viral component directly from inoculated VTM without RNA extraction. Inoculated VTMs of already tested 50 positive and 50 negative samples were divided into three groups. Group I was treated with Proteinase K (PK) followed by 3-step-heat treatment at different temperatures (25°C, 60°C, and 98°C) and stored at 4°C. Group II was directly subjected to 3-step-heat treatment without PK exposure and stored at 4°C. And group III was set-up as standard group; it was processed using Qiagen's column based QIAamp Nucleic Acid kit and the obtained nucleic acids were stored at 4°C. These stored samples were used as a template to execute real-time polymerase chain reaction, and results were noted. Group I demonstrated 96% and 88% sensitivity for N and ORF1ab genes respectively, whereas group II demonstrated 78% and 60% when compared to the results of standard group III. Overall group I showed better results than group II when compared to group III. Thus, in situations where gold-standard reagents are not available, PK exposure and heat treatment can be employed to carry out molecular detection of SARS-CoV2 viral component.


Assuntos
COVID-19 , RNA Viral , Endopeptidase K , Humanos , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real , SARS-CoV-2
19.
Emerg Infect Dis ; 27(12): 3178-3180, 2021 12.
Artigo em Inglês | MEDLINE | ID: covidwho-1528802

RESUMO

In vitro determination of severe acute respiratory syndrome coronavirus 2 neutralizing antibodies induced in serum samples from recipients of the CoronaVac vaccine showed a short protection period against the original virus strain and limited protection against variants of concern. These data provide support for vaccine boosters, especially variants of concern circulate.


Assuntos
Anticorpos Neutralizantes , COVID-19 , Anticorpos Antivirais , Vacinas contra COVID-19 , Humanos , SARS-CoV-2
20.
Emerg Infect Dis ; 27(12): 3175-3178, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: covidwho-1528801

RESUMO

We conducted a multi-institutional study in Taiwan and a systematic review of the literature for reports of Guillain-​Barré syndrome after coronavirus disease vaccination. This condition, mostly the classic form and the acute inflammatory demyelinating polyneuropathy subtype, has been reported in 39 cases and has occurred within 2 weeks of vaccine administration.


Assuntos
COVID-19 , Síndrome de Guillain-Barré , Vacinas contra COVID-19 , Síndrome de Guillain-Barré/etiologia , Humanos , SARS-CoV-2 , Vacinação
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...