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Polymeric nanoencapsulation of alpha interferon increases drug bioavailability and induces a sustained antiviral response in vivo.
Pedroso-Santana, Seidy; Lamazares Arcia, Emilio; Fleitas-Salazar, Noralvis; Gancino Guevara, Marlon; Mansilla, Rodrigo; Gómez-Gaete, Carolina; Altamirano, Claudia; Fernandez, Katherina; Ruiz, Alvaro; Toledo Alonso, Jorge R.
  • Pedroso-Santana S; Laboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción CP. 4030000, Chile.
  • Lamazares Arcia E; Laboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción CP. 4030000, Chile; Centro de Biotecnología y Biomedicina SpA, Granada 168, Vilumanque, Concepción, Chile.
  • Fleitas-Salazar N; Laboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción CP. 4030000, Chile; Centro de Biotecnología y Biomedicina SpA, Granada 168, Vilumanque, Concepción, Chile.
  • Gancino Guevara M; Laboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción CP. 4030000, Chile.
  • Mansilla R; Laboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción CP. 4030000, Chile; Centro de Biotecnología y Biomedicina SpA, Granada 168, Vilumanque, Concepción, Chile.
  • Gómez-Gaete C; Departamento de Farmacia, Facultad de Farmacia, Universidad de Concepción, Barrio Universitario s/n, Concepción CP. 4030000, Chile; Unidad de Desarrollo Tecnológico UDT, Universidad de Concepción, Chile.
  • Altamirano C; Biochemical Engineering School, Pontificia Universidad Católica de Valparaíso, Av. Brasil 2950, CP. 2390302, Chile.
  • Fernandez K; Laboratory of Biomaterials, Departamento de Ingeniería Química, Facultad de Ingeniería, University of Concepción, Barrio Universitario s/n, Concepción CP. 4030000, Chile.
  • Ruiz A; Pathology and Preventive Medicine Department, School of Veterinary Sciences, Universidad de Concepción, Av. Vicente Méndez 595, Chillan CP. 3780000, Chile.
  • Toledo Alonso JR; Laboratorio de Biotecnología y Biofármacos, Departamento de Fisiopatología, Facultad de Ciencias Biológicas, Universidad de Concepción, Barrio Universitario s/n, Concepción CP. 4030000, Chile; Centro de Biotecnología y Biomedicina SpA, Granada 168, Vilumanque, Concepción, Chile. Electronic address:
Mater Sci Eng C Mater Biol Appl ; 116: 111260, 2020 Nov.
Article in English | MEDLINE | ID: covidwho-1452344
ABSTRACT
Polymeric nanoparticulate systems allow the encapsulation of bio-active substances, giving them protection against external agents and increasing the drug's bioavailability. The use of biocompatible and biodegradable polymers usually guarantees the harmless character of the formulation, and a controlled drug release is also assured. A relatively easy procedure to obtain polymeric formulations of bioactive agents is ionotropic gelation, which allows the synthesis of chitosan (CS) - sodium tri-polyphosphate nanoparticles (NPs) loading encapsulated proteins. In this work, Bovine serum albumin (BSA) model protein and a recombinant porcine alpha interferon variant were used to obtain nanoparticulate formulations. The internalization of the encapsulated material by cells was studied using a BSA-fluorescein system; the fluorescent conjugate was observable inside the cells after 20 h of incubation. The therapeutic CS-alpha interferon formulation showed a maximum of protein released in vitro at around 90 h. This system was found to be safe in a cytotoxicity assay, while biological activity experiments in vitro showed antiviral protection of cells in the presence of encapsulated porcine alpha interferon. In vivo experiments in pigs revealed a significant and sustained antiviral response through overexpression of the antiviral markers OAS2 and PKR. This proves the preservation of porcine alpha interferon biological activity, and also that a lasting response was obtained. This procedure is an effective and safe method to formulate drugs in nanoparticulate systems, representing a significant contribution to the search for more effective drug delivery strategies.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pharmaceutical Preparations / Chitosan / Nanoparticles Type of study: Experimental Studies / Prognostic study Topics: Variants Limits: Animals Language: English Journal: Mater Sci Eng C Mater Biol Appl Year: 2020 Document Type: Article Affiliation country: J.msec.2020.111260

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Pharmaceutical Preparations / Chitosan / Nanoparticles Type of study: Experimental Studies / Prognostic study Topics: Variants Limits: Animals Language: English Journal: Mater Sci Eng C Mater Biol Appl Year: 2020 Document Type: Article Affiliation country: J.msec.2020.111260