Clinical severe acute respiratory syndrome coronavirus 2 isolation and antiviral testing.
Antivir Chem Chemother
; 29: 20402066211061063, 2021.
Article
in English
| MEDLINE | ID: covidwho-1528665
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 is an RNA virus currently causing a pandemic. Due to errors during replication, mutations can occur and result in cell adaptation by the virus or in the rise of new variants. This can change the attachment receptors' usage, result in different morphology of plaques, and can affect as well antiviral development. Indeed, a molecule can be active on laboratory strains but not necessarily on circulating strains or be effective only against some viral variants. Experiments with clinical samples with limited cell adaptation should be performed to confirm the efficiency of drugs of interest. In this protocol, we present a method to culture severe acute respiratory syndrome coronavirus 2 from nasopharyngeal swabs, obtain a high viral titer while limiting cell adaptation, and assess antiviral efficiency.
Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Antiviral Agents
/
COVID-19
Type of study:
Prognostic study
Topics:
Variants
Limits:
Humans
Language:
English
Journal:
Antivir Chem Chemother
Journal subject:
Chemistry
/
Drug Therapy
/
Virology
Year:
2021
Document Type:
Article
Affiliation country:
20402066211061063
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