Proteomic landscape of SARS-CoV-2- and MERS-CoV-infected primary human renal epithelial cells.
Life Sci Alliance
; 5(5)2022 05.
Article
in English
| MEDLINE | ID: covidwho-1675573
ABSTRACT
Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type-specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection-induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type-specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication.
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Proteome
/
Proteomics
/
Epithelial Cells
/
Middle East Respiratory Syndrome Coronavirus
/
SARS-CoV-2
/
Kidney
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Year:
2022
Document Type:
Article
Affiliation country:
Lsa.202201371
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