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MALDI-TOF Mass Spectrometric Detection of SARS-CoV-2 Using Cellulose Sulfate Ester Enrichment and Hot Acid Treatment.
Chen, Dapeng; Bryden, Wayne A; Fenselau, Catherine; McLoughlin, Michael; Haddaway, Caroline R; Devin, Alese P; Caton, Emily R; Bradrick, Shelton S; Miller, Joy M; Tacheny, Erin A; Lemmon, M Megan; Bogan, Joseph.
  • Chen D; Zeteo Tech, Inc., Sykesville, Maryland 21784, United States.
  • Bryden WA; Zeteo Tech, Inc., Sykesville, Maryland 21784, United States.
  • Fenselau C; Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, United States.
  • McLoughlin M; Zeteo Tech, Inc., Sykesville, Maryland 21784, United States.
  • Haddaway CR; Zeteo Tech, Inc., Sykesville, Maryland 21784, United States.
  • Devin AP; Zeteo Tech, Inc., Sykesville, Maryland 21784, United States.
  • Caton ER; Zeteo Tech, Inc., Sykesville, Maryland 21784, United States.
  • Bradrick SS; MRIGlobal, Kansas City, Missouri 64110, United States.
  • Miller JM; MRIGlobal, Kansas City, Missouri 64110, United States.
  • Tacheny EA; MRIGlobal, Gaithersburg, Maryland 20878, United States.
  • Lemmon MM; MRIGlobal, Gaithersburg, Maryland 20878, United States.
  • Bogan J; MRIGlobal, Gaithersburg, Maryland 20878, United States.
J Proteome Res ; 21(8): 2055-2062, 2022 08 05.
Article in English | MEDLINE | ID: covidwho-1921546
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the ongoing coronavirus disease 2019 (COVID-19) pandemic. Here we report a novel strategy for the rapid detection of SARS-CoV-2 based on an enrichment approach exploiting the affinity between the virus and cellulose sulfate ester functional groups, hot acid hydrolysis, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS). Virus samples were enriched using cellulose sulfate ester microcolumns. Virus peptides were prepared using the hot acid aspartate-selective hydrolysis and characterized by MALDI-TOF MS. Collected spectra were processed with a peptide fingerprint algorithm, and searching parameters were optimized for the detection of SARS-CoV-2. These peptides provide high sequence coverage for nucleocapsid (N protein) and allow confident identification of SARS-CoV-2. Peptide markers contributing to the detection were rigorously identified using bottom-up proteomics. The approach demonstrated in this study holds the potential for developing a rapid assay for COVID-19 diagnosis and detecting virus variants from a variety of sources, such as sewage and nasal swabs.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Topics: Variants Limits: Humans Language: English Journal: J Proteome Res Journal subject: Biochemistry Year: 2022 Document Type: Article Affiliation country: Acs.jproteome.2c00238

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Full text: Available Collection: International databases Database: MEDLINE Main subject: SARS-CoV-2 / COVID-19 Type of study: Diagnostic study / Experimental Studies / Randomized controlled trials Topics: Variants Limits: Humans Language: English Journal: J Proteome Res Journal subject: Biochemistry Year: 2022 Document Type: Article Affiliation country: Acs.jproteome.2c00238