Association of perturbation of oral bacterial with incident of Alzheimer's disease: A pilot study.
J Clin Lab Anal
; 36(7): e24483, 2022 Jul.
Article
in English
| MEDLINE | ID: covidwho-1929823
ABSTRACT
OBJECTIVE:
This case-control study was designed to compare the composition of the predominant oral bacterial microbiome in Alzheimer's disease (AD) and control group. SUBJECT A total of 30 adult participants (15 AD and 15 healthy individuals) were entered in this study. The composition of oral bacterial microbiome was examined by quantitative real-time polymerase chain reaction (qPCR) using bacterial 16S rDNA gene. The levels of systemic inflammatory cytokines in both groups were assessed using enzyme-linked immunosorbent assays (ELISA).RESULTS:
The loads of Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia were significantly more abundant in the AD compared to the control group (p < 0.05). Although Aggregatibacter actinomycetemcomitans and Streptococcus mutans were relatively frequent in the AD group, no significance difference was observed in their copy number between two groups. Although the concentrations of IL-1, IL-6, and TNF-α were higher in the AD group, there was a significant difference in their levels between the two groups (p < 0.05). Finally, there was a significant relationship between increased number of pathogenic bacteria in oral microbiome and higher concentration of cytokines in patient's blood.CONCLUSION:
Our knowledge of oral microbiome and its exact association with AD is rather limited; our study showed a significant association between changes in oral microbiome bacteria, increased inflammatory cytokines, and AD.Keywords
Full text:
Available
Collection:
International databases
Database:
MEDLINE
Main subject:
Alzheimer Disease
/
Microbiota
/
Mouth
Type of study:
Experimental Studies
/
Observational study
/
Randomized controlled trials
Limits:
Adult
/
Humans
Language:
English
Journal:
J Clin Lab Anal
Journal subject:
Laboratory Techniques and procedures
Year:
2022
Document Type:
Article
Affiliation country:
Jcla.24483
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