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Investigation of potential inhibitor properties of violacein against HIV-1 RT and CoV-2 Spike RBD:ACE-2.
Doganci, Merve Ayse; Ay Sal, Fulya; Guler, Halil Ibrahim; Kati, Hatice; Ceylan, Esma; Belduz, Ali Osman; Bozdal, Gozde; Yayli, Nurettin; Canakci, Sabriye.
  • Doganci MA; Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
  • Ay Sal F; Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
  • Guler HI; Department of Molecular Biology and Genetics, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
  • Kati H; Department of Biology, Faculty of Arts and Sciencies, Giresun University, 28200, Giresun, Turkey.
  • Ceylan E; Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
  • Belduz AO; Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
  • Bozdal G; Department of Pharmacognosis, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
  • Yayli N; Department of Pharmacognosis, Faculty of Pharmacy, Karadeniz Technical University, 61080, Trabzon, Turkey.
  • Canakci S; Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey. sabriye@ktu.edu.tr.
World J Microbiol Biotechnol ; 38(9): 161, 2022 Jul 14.
Article in English | MEDLINE | ID: covidwho-1930506
ABSTRACT
A violacein-producing bacterium was isolated from a mud sample collected near a hot spring on Kümbet Plateau in Giresun Province and named the GK strain. According to the phylogenetic tree constructed using 16S rRNA gene sequence analysis, the GK strain was identified and named Janthinobacterium sp. GK. The crude violacein pigments were separated into three different bands on a TLC sheet. Then violacein and deoxyviolacein were purified by vacuum liquid column chromatography and identified by NMR spectroscopy. According to the inhibition studies, the HIV-1 RT inhibition rate of 1 mM violacein from the GK strain was 94.28% and the CoV-2 spike RBDACE2 inhibition rate of 2 mM violacein was 53%. In silico studies were conducted to investigate the possible interactions between violacein and deoxyviolacein and three reference molecules with the target proteins angiotensin-converting enzyme 2 (ACE2), HIV-1 reverse transcriptase, and SARS-CoV-2 spike receptor binding domain. Ligand violacein binds strongly to the receptor ACE2, HIV-1 reverse transcriptase, and SARS-CoV-2 spike receptor binding domain with a binding energy of -9.94 kcal/mol, -9.32 kcal/mol, and -8.27 kcal/mol, respectively. Deoxyviolacein strongly binds to the ACE2, HIV-1 reverse transcriptase, and SARS-CoV-2 spike receptor binding domain with a binding energy of -10.38 kcal/mol, -9.50 kcal/mol, and -8.06 kcal/mol, respectively. According to these data, violacein and deoxyviolacein bind to all the receptors quite effectively. SARS-CoV-2 spike protein and HIV-1-RT inhibition studies with violacein and deoxyviolacein were performed for the first time in the literature.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: HIV-1 / Spike Glycoprotein, Coronavirus / Angiotensin-Converting Enzyme 2 / COVID-19 / Indoles Type of study: Randomized controlled trials Language: English Journal: World J Microbiol Biotechnol Year: 2022 Document Type: Article Affiliation country: S11274-022-03350-0

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Full text: Available Collection: International databases Database: MEDLINE Main subject: HIV-1 / Spike Glycoprotein, Coronavirus / Angiotensin-Converting Enzyme 2 / COVID-19 / Indoles Type of study: Randomized controlled trials Language: English Journal: World J Microbiol Biotechnol Year: 2022 Document Type: Article Affiliation country: S11274-022-03350-0