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Porcine epidemic diarrhea virus strain FJzz1 infection induces type I/III IFNs production through RLRs and TLRs-mediated signaling.
Chen, Pengfei; Zhu, Junrui; Yu, Jiarong; Liu, Ruilin; Lao, Mengqin; Yu, Lingxue; Gao, Fei; Jiang, Yifeng; Liu, Changlong; Tong, Wu; Liu, Huili; Tong, Guangzhi; Zhou, Yanjun.
  • Chen P; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Zhu J; Institute of Animal Husbandry and Veterinary, Shanghai Academy of Agricultural Science, Shanghai, China.
  • Yu J; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Liu R; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Lao M; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Yu L; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Gao F; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Jiang Y; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Liu C; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Tong W; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Liu H; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
  • Tong G; Institute of Animal Husbandry and Veterinary, Shanghai Academy of Agricultural Science, Shanghai, China.
  • Zhou Y; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Front Immunol ; 13: 984448, 2022.
Article in English | MEDLINE | ID: covidwho-1987499
ABSTRACT
Interferons (IFNs) including type I/III IFNs are the major components of the host innate immune response against porcine epidemic diarrhea virus (PEDV) infection, and several viral proteins have been identified to antagonize type I/III IFNs productions through diverse strategies. However, the modulation of PEDV infection upon the activation of the host's innate immune response has not been fully characterized. In this study, we observed that various IFN-stimulated genes (ISGs) were upregulated significantly in a time- and dose-dependent manner in LLC-PK1 cells infected with the PEDV G2 strain FJzz1. The transcriptions of IRF9 and STAT1 were increased markedly in the late stage of FJzz1 infection and the promotion of the phosphorylation and nuclear translocation of STAT1, implicating the activation of the JAK-STAT signaling pathway during FJzz1 infection. In addition, abundant type I/III IFNs were produced after FJzz1 infection. However, type I/III IFNs and ISGs decreased greatly in FJzz1-infected LLC-PK1 cells following the silencing of the RIG-I-like receptors (RLRs), including RIG-I and MDA5, and the Toll-like receptors (TLRs) adaptors, MyD88 and TRIF. Altogether, FJzz1 infection induces the production of type-I/III IFNs in LLC-PK1 cells, in which RLRs and TLRs signaling pathways are involved, followed by the activation of the JAK-STAT signaling cascade, triggering the production of numerous ISGs to exert antiviral effects of innate immunity.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Interferon Type I / Porcine epidemic diarrhea virus Limits: Animals Language: English Journal: Front Immunol Year: 2022 Document Type: Article Affiliation country: Fimmu.2022.984448

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Interferon Type I / Porcine epidemic diarrhea virus Limits: Animals Language: English Journal: Front Immunol Year: 2022 Document Type: Article Affiliation country: Fimmu.2022.984448