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In vitro inhibition of SARS-CoV-2 Infection by dry algae powders.
Garcia-Ruiz, Daniel; Villalobos-Sánchez, Erendira; Alam-Escamilla, David; Elizondo-Quiroga, Darwin.
  • Garcia-Ruiz D; Medical and Pharmaceutical Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco (CIATEJ), Av. Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
  • Villalobos-Sánchez E; Medical and Pharmaceutical Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco (CIATEJ), Av. Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico.
  • Alam-Escamilla D; CREAMOS MAS S.A. DE C.V, Calle Monte Albán No. 965. Colonia. Independencia Oriente, C.P. 44340, Guadalajara, Jalisco, Mexico.
  • Elizondo-Quiroga D; Medical and Pharmaceutical Biotechnology Unit, Center for Research and Assistance in Technology and Design of the State of Jalisco (CIATEJ), Av. Normalistas 800, Colinas de la Normal, 44270, Guadalajara, Jalisco, Mexico. delizondo@ciatej.mx.
Sci Rep ; 12(1): 17101, 2022 Oct 12.
Article in English | MEDLINE | ID: covidwho-2062276
ABSTRACT
Chlorella spp., Spirulina spp., and fucoidan dry powders, are commercialized as food supplements and are considered safe for human consumption. Their broad-spectrum antiviral properties have been studied, however, their effect against SARS-CoV-2 remains unknown. We investigated the potential antiviral activity of three algae powders Chlorella vulgaris, Arthrospira maxima (Spirulina) and fucoidan purified from marine brown algae Sargassum spp. against SARS-CoV-2 infection in vitro. Vero cells were incubated with 70 µg/ml of each algae powder and either 50 or 100 TCID50/ml of SARS-CoV-2, in two types of experiments (pretreatment and simultaneous) and comparing two kinds of solvents (DMEM and DMSO). Chlorella vulgaris powder, inhibited SARS-CoV-2 infection in all assays; viral RNA was significantly reduced in supernatants at 24, 48, 72, and 96 h post-infection, the highest difference in viral load (8000-fold) was observed after 96 h. Arthrospira maxima powder inhibited SARS-CoV-2 infection using 50 TCID50/ml for both experimental schemes, but protection percent was lower when viral inoculum was increase to 100 TCID50/ml; viral RNA decreased 48 h after infection, reaching a 250-fold difference at 72 h. Fucoidan powder partially inhibited SARS-CoV-2 infection since no CPE was observed in 62.5% of trated cultures in DMEM, but the antiviral activity was increased to 100% of protection when DMSO was used as solvent. All the algae samples showed high antiviral activity against SARS-CoV-2 with a SI above of 18. These results suggest that all three algae samples are potential therapeutic candidates for the treatment of COVID-19.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Chlorella vulgaris / COVID-19 Drug Treatment Limits: Animals / Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-22148-6

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Chlorella vulgaris / COVID-19 Drug Treatment Limits: Animals / Humans Language: English Journal: Sci Rep Year: 2022 Document Type: Article Affiliation country: S41598-022-22148-6