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Virological and Serological Assessment of US Army Trainees Isolated for Coronavirus Disease 2019.
Hakre, Shilpa; Lakhal-Naouar, Ines; King, David B; Burns, Jennifer L; Jackson, Kenya N; Krauss, Stephen W; Chandrasekaran, Prabha; McCauley, Melanie D; Ober Shepherd, Brittany L; McHenry, Samantha; Bianchi, Elizabeth J; Ouellette, Jason; Darden, Janice M; Sanborn, Aaron D; Daye, Sharon P; Kwon, Paul O; Stubbs, Jeremiah; Brigantti, Crystal L; Hall, Tara L; Beagle, Milford H; Pieri, Jason A; Frambes, Timothy R; O'Connell, Robert J; Modjarrad, Kayvon; Murray, Clinton K; Jagodzinski, Linda L; Scott, Paul T; Peel, Sheila A.
  • Hakre S; Walter Reed Army Institute of Research, Emerging Infectious Diseases Branch, Silver Spring, Maryland, USA.
  • Lakhal-Naouar I; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • King DB; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • Burns JL; Walter Reed Army Institute of Research, Diagnostics and Countermeasures Branch, Silver Spring, Maryland, USA.
  • Jackson KN; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • Krauss SW; Walter Reed Army Institute of Research, Pilot Bioproduction Facility, Silver Spring, Maryland, USA.
  • Chandrasekaran P; Walter Reed Army Institute of Research, Experimental Therapeutics, Silver Spring, Maryland, USA.
  • McCauley MD; Walter Reed Army Institute of Research, Center for Military Psychiatry and Neuroscience, Silver Spring, Maryland, USA.
  • Ober Shepherd BL; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • McHenry S; Walter Reed Army Institute of Research, Diagnostics and Countermeasures Branch, Silver Spring, Maryland, USA.
  • Bianchi EJ; Walter Reed Army Institute of Research, Emerging Infectious Diseases Branch, Silver Spring, Maryland, USA.
  • Ouellette J; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • Darden JM; Walter Reed Army Institute of Research, Emerging Infectious Diseases Branch, Silver Spring, Maryland, USA.
  • Sanborn AD; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • Daye SP; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • Kwon PO; Walter Reed Army Institute of Research, Emerging Infectious Diseases Branch, Silver Spring, Maryland, USA.
  • Stubbs J; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • Brigantti CL; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • Hall TL; Walter Reed Army Institute of Research, Diagnostics and Countermeasures Branch, Silver Spring, Maryland, USA.
  • Beagle MH; Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MarylandUSA.
  • Pieri JA; Walter Reed Army Institute of Research, Diagnostics and Countermeasures Branch, Silver Spring, Maryland, USA.
  • Frambes TR; Walter Reed Army Institute of Research, Clinical Trials Center, Silver Spring, Maryland, USA.
  • O'Connell RJ; Walter Reed Army Institute of Research, One Health Branch, Silver Spring, Maryland, USA.
  • Modjarrad K; Program Executive Office for Simulation, Training and Instrumentation, Orlando, Florida, USA.
  • Murray CK; Fort Belvoir, Occupational Medicine, Virginia, USA.
  • Jagodzinski LL; DiLorenzo Pentagon Health Clinic, Optometry, Washington, District of Columbia, USA.
  • Scott PT; Moncrief Army Health Clinic, Fort Jackson, South Carolina, USA.
  • Peel SA; 10th Mountain Division, Fort Drum, New York, USA.
J Infect Dis ; 226(10): 1743-1752, 2022 Nov 11.
Article in English | MEDLINE | ID: covidwho-2121302
ABSTRACT

BACKGROUND:

Laboratory screening for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a key mitigation measure to avoid the spread of infection among recruits starting basic combat training in a congregate setting. Because viral nucleic acid can be detected persistently after recovery, we evaluated other laboratory markers to distinguish recruits who could proceed with training from those who were infected.

METHODS:

Recruits isolated for coronavirus disease 2019 (COVID-19) were serially tested for SARS-CoV-2 subgenomic ribonucleic acid (sgRNA), and viral load (VL) by reverse-transcriptase polymerase chain reaction (RT-PCR), and for anti- SARS-CoV-2. Cluster and quadratic discriminant analyses of results were performed.

RESULTS:

Among 229 recruits isolated for COVID-19, those with a RT-PCR cycle threshold >30.49 (sensitivity 95%, specificity 96%) or having sgRNA log10 RNA copies/mL <3.09 (sensitivity and specificity 96%) at entry into isolation were likely SARS-CoV-2 uninfected. Viral load >4.58 log10 RNA copies/mL or anti-SARS-CoV-2 signal-to-cutoff ratio <1.38 (VL sensitivity and specificity 93%; anti-SARS-CoV-2 sensitivity 83%, specificity 79%) had comparatively lower sensitivity and specificity when used alone for discrimination of infected from uninfected.

CONCLUSIONS:

Orthogonal laboratory assays used in combination with RT-PCR may have utility in determining SARS-CoV-2 infection status for decisions regarding isolation.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Long Covid Limits: Humans Language: English Journal: J Infect Dis Year: 2022 Document Type: Article Affiliation country: Infdis

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Full text: Available Collection: International databases Database: MEDLINE Main subject: COVID-19 Type of study: Diagnostic study / Experimental Studies Topics: Long Covid Limits: Humans Language: English Journal: J Infect Dis Year: 2022 Document Type: Article Affiliation country: Infdis