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Xuanfei Baidu Formula attenuates LPS-induced acute lung injury by inhibiting the NF-κB signaling pathway.
Zhu, Yanru; Luo, Lifei; Zhang, Meng; Song, Xinbo; Wang, Ping; Zhang, Han; Zhang, Jingze; Liu, Dailin.
  • Zhu Y; State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; Tianjin Modern Innovation Chinese Medicine Technology Co., Ltd., Tianjin, China.
  • Luo L; State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; Tianjin Modern Innovation Chinese Medicine Technology Co., Ltd., Tianjin, China.
  • Zhang M; State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; Tianjin Modern Innovation Chinese Medicine Technology Co., Ltd., Tianjin, China.
  • Song X; State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; Tianjin Modern Innovation Chinese Medicine Technology Co., Ltd., Tianjin, China.
  • Wang P; Tianjin Modern Innovation Chinese Medicine Technology Co., Ltd., Tianjin, China.
  • Zhang H; State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
  • Zhang J; Tianjin Modern Innovation Chinese Medicine Technology Co., Ltd., Tianjin, China.
  • Liu D; State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; Tianjin Modern Innovation Chinese Medicine Technology Co., Ltd., Tianjin, China. Electronic address: dailinlch@163.com.
J Ethnopharmacol ; 301: 115833, 2023 Jan 30.
Article in English | MEDLINE | ID: covidwho-2131464
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19. Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients. However, the active ingredients of XFBD have not been extensively studied, and its mechanism of action in the treatment of ALI is not well understood. AIM OF THE STUDY The purpose of this study was to investigate the mechanism of action of XFBD in treating ALI in rats, by evaluating its active components. MATERIALS AND

METHODS:

Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry. The potential targets of XFBD for ALI treatment were predicted using network pharmacological analysis. Finally, the molecular mechanism of XFBD was validated using a RAW264.7 cell inflammation model and a mouse ALI model.

RESULTS:

A total of 113 compounds were identified in XFBD. Network pharmacology revealed 34 hub targets between the 113 compounds and ALI. The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI. We found that XFBD reduced proinflammatory factor levels in LPS-induced cellular models. By examining the lung wet/dry weight ratio and pathological sections in vivo, XFBD was found that XFBD could alleviate ALI. Immunohistochemistry results showed that XFBD inhibited ALI-induced increases in p-IKK, p-NF-κB p65, and iNOS proteins. In vitro experiments demonstrated that XFBD inhibited LPS-induced activation of the NF-κB pathway.

CONCLUSION:

This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
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Full text: Available Collection: International databases Database: MEDLINE Main subject: Acute Lung Injury / COVID-19 Type of study: Experimental Studies / Prognostic study Topics: Traditional medicine Limits: Animals Language: English Journal: J Ethnopharmacol Year: 2023 Document Type: Article Affiliation country: J.jep.2022.115833

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Full text: Available Collection: International databases Database: MEDLINE Main subject: Acute Lung Injury / COVID-19 Type of study: Experimental Studies / Prognostic study Topics: Traditional medicine Limits: Animals Language: English Journal: J Ethnopharmacol Year: 2023 Document Type: Article Affiliation country: J.jep.2022.115833