Production of the Receptor-binding Domain of the Viral Spike Proteins from 2003 and 2019 SARS CoVs and the Four Common Human Coronaviruses for Serologic Assays and Inhibitor Screening.
Bio Protoc
; 11(10): e4026, 2021 May 20.
Article
in English
| MEDLINE | ID: covidwho-2326148
ABSTRACT
The recombinant receptor-binding domain (RBD) of the viral spike protein from SARS-CoV-1 and 2 are reliable antigens for detecting viral-specific antibodies in humans. We and others have shown that the levels of RBD-binding antibodies and SARS-CoV-2 neutralizing antibodies in patients are correlated. Here, we report the expression and purification of properly folded RBD proteins from SARS and common-cold HCoVs in mammalian cells. RBD proteins were produced with cleavable tags for affinity purification from the cell culture medium and to support multiple immunoassay platforms and drug discovery efforts. Graphic abstract High-Yield Production of Viral Spike RBDs for Diagnostics and Drug Discovery.
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Collection:
International databases
Database:
MEDLINE
Language:
English
Journal:
Bio Protoc
Year:
2021
Document Type:
Article
Affiliation country:
BioProtoc.4026
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