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A Serological Snapshot of COVID-19 Initial Stages in Israel by a 6-Plex Antigen Array.
Fisher, Morly; Levy, Haim; Fatelevich, Ella; Afrimov, Yafa; Ben-Shmuel, Amir; Rosenfeld, Ronit; Noy-Porat, Tal; Glinert, Itai; Sittner, Assa; Biber, Asaf; Belkin, Ana; Bar-David, Elad; Puni, Reut; Levy, Itzchak; Mazor, Ohad; Weiss, Shay; Mechaly, Adva.
  • Fisher M; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Levy H; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Fatelevich E; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Afrimov Y; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Ben-Shmuel A; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Rosenfeld R; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Noy-Porat T; Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Glinert I; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Sittner A; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Biber A; Sheba Medical Centergrid.413795.d and the Sackler Medical School, Tel Aviv University, Tel Aviv-Yafo, Israel.
  • Belkin A; Sheba Medical Centergrid.413795.d and the Sackler Medical School, Tel Aviv University, Tel Aviv-Yafo, Israel.
  • Bar-David E; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Puni R; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Levy I; Sheba Medical Centergrid.413795.d and the Sackler Medical School, Tel Aviv University, Tel Aviv-Yafo, Israel.
  • Mazor O; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Weiss S; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
  • Mechaly A; Department of Infectious Diseases, Israel Institute for Biological Researchgrid.419290.7, Ness-Ziona, Israel.
Microbiol Spectr ; 9(2): e0087021, 2021 10 31.
Artigo em Inglês | MEDLINE | ID: covidwho-1455682
ABSTRACT
The first case of SARS-CoV-2 was discovered in Israel in late February 2020. Three major outbreaks followed, resulting in over 800,000 cases and over 6,000 deaths by April 2021. Our aim was characterization of a serological snapshot of Israeli patients and healthy adults in the early months of the COVID-19 pandemic. Sera from 55 symptomatic COVID-19 patients and 146 healthy subjects (early-pandemic, reverse transcription-quantitative PCR [qRT-PCR]-negative), collected in Israel between March and April 2020, were screened for SARS-CoV-2-specific IgG, IgM, and IgA antibodies, using a 6-plex antigen microarray presenting the whole inactivated virus and five viral antigens a stabilized version of the spike ectodomain (S2P), spike subunit 1 (S1), receptor-binding-domain (RBD), N-terminal-domain (NTD), and nucleocapsid (NC). COVID-19 patients, 4 to 40 days post symptom onset, presented specific IgG to all of the viral antigens (6/6) in 54 of the 55 samples (98% sensitivity). Specific IgM and IgA antibodies for all six antigens were detected in only 10% (5/55) and 4% (2/55) of the patients, respectively, suggesting that specific IgG is a superior serological marker for COVID-19. None of the qRT-PCR-negative sera reacted with all six viral antigens (100% specificity), and 48% (70/146) were negative throughout the panel. Our findings confirm a low seroprevalence of anti-SARS-CoV-2 antibodies in the Israeli adult population prior to the COVID-19 outbreak. We further suggest that the presence of low-level cross-reacting antibodies in naive individuals calls for a combined, multiantigen analysis for accurate discrimination between naive and exposed individuals. IMPORTANCE A 6-plex protein array presenting the whole inactivated virus and five nucleocapsid and spike-derived SARS-CoV-2 antigens was used to generate a serological snapshot of SARS-CoV-2 seroprevalence and seroconversion in Israel in the early months of the pandemic. Our findings confirm a very low seroprevalence of anti-SARS-CoV-2 antibodies in the Israeli adult population. We further propose that the presence of low-level nonspecific antibodies in naive individuals calls for a combined, multiantigen analysis for accurate discrimination between naive and exposed individuals enabling accurate determination of seroconversion. The developed assay is currently applied to evaluate immune responses to the Israeli vaccine during human phase I/II trials.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Análise em Microsséries / Teste Sorológico para COVID-19 / SARS-CoV-2 / COVID-19 / Anticorpos Antivirais Tipo de estudo: Estudo diagnóstico / Estudo experimental / Estudo observacional / Ensaios controlados aleatorizados Tópicos: Covid persistente / Vacinas Limite: Adulto / Idoso / Feminino / Humanos / Masculino / Meia-Idade / Jovem adulto País/Região como assunto: Ásia Idioma: Inglês Revista: Microbiol Spectr Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Spectrum.00870-21

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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Análise em Microsséries / Teste Sorológico para COVID-19 / SARS-CoV-2 / COVID-19 / Anticorpos Antivirais Tipo de estudo: Estudo diagnóstico / Estudo experimental / Estudo observacional / Ensaios controlados aleatorizados Tópicos: Covid persistente / Vacinas Limite: Adulto / Idoso / Feminino / Humanos / Masculino / Meia-Idade / Jovem adulto País/Região como assunto: Ásia Idioma: Inglês Revista: Microbiol Spectr Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: Spectrum.00870-21