Polyaniline-based electrochemical immunosensor for the determination of antibodies against SARS-CoV-2 spike protein.
Sci Total Environ
; 862: 160700, 2023 Mar 01.
Artigo
em Inglês
| MEDLINE | ID: covidwho-2150569
ABSTRACT
In this work, we report an impedimetric system for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Spike protein. The sensing platform is based on recombinant Spike protein (SCoV2-rS) immobilized on the phytic acid doped polyaniline films (PANI-PA). The affinity interaction between immobilized SCoV2-rS protein and antibodies in the physiological range of concentrations was registered by electrochemical impedance spectroscopy. Analytical parameters of the sensing platform were tuned by the variation of electropolymerization times during the synthesis of PANI-PA films. The lowest limit of detection and quantification were obtained for electropolymerization time of 20 min and equalled 8.00 ± 0.20 nM and 23.93 ± 0.60 nM with an equilibrium dissociation constant of 3 nM. The presented sensing system is label-free and suitable for the direct detection of antibodies against SARS-CoV-2 in real patient serum samples after coronavirus disease 2019 and/or vaccination.
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Texto completo:
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Coleções:
Bases de dados internacionais
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
COVID-19
Tópicos:
Vacinas
Limite:
Humanos
Idioma:
Inglês
Revista:
Sci Total Environ
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
J.scitotenv.2022.160700
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