Your browser doesn't support javascript.
Development and validation of molnupiravir assessment in bulk powder and pharmaceutical formulation by the RP-HPLC-UV method.
Annadi, Abdelaziz M; El Zahar, Noha M; El-Din A Abdel-Sattar, Nour; Mohamed, Ekram H; Mahmoud, Safwat A; Attia, Mohamed S.
  • Annadi AM; Chemistry Department, Faculty of Science, Ain Shams University Abbassia Cairo 11566 Egypt mohd_mostafa@sci.asu.edu.eg.
  • El Zahar NM; Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Organization of African Unity Street Abbassia Cairo 11566 Egypt.
  • El-Din A Abdel-Sattar N; Medicinal Chemistry Department, Faculty of Pharmacy, King Salman International University Ras-Sedr, South Sinai Egypt.
  • Mohamed EH; Chemistry Department, Faculty of Science, Ain Shams University Abbassia Cairo 11566 Egypt mohd_mostafa@sci.asu.edu.eg.
  • Mahmoud SA; Pharmaceutical Chemistry Department, Faculty of Pharmacy, The British University in Egypt ElSherouk City Cairo Egypt.
  • Attia MS; Physics Department, Faculty of Science, Northern Border University Arar Saudi Arabia samahmoud2002@yahoo.com.
RSC Adv ; 12(53): 34512-34519, 2022 Nov 29.
Artigo em Inglês | MEDLINE | ID: covidwho-2160359
ABSTRACT
An accurate, sensitive and selective RP-HPLC-UV method has been established for the estimation of Molnupiravir (MOL) in pure bulk powder and pharmaceutical formulation. Separation was achieved on an Inertsil C18 column (150.0 mm × 4.6 mm, 5.0 µm), using a mobile phase of 20 mM phosphate buffer pH 2.5 acetonitrile (80 20, v/v%) in isocratic mode with a flow rate of 1.0 mL min-1. The λ max of MOL prepared in the chosen diluent (ethanol water in equal proportions) was found to be 230.0 nm. The constructed calibration curve was found to be linear in the concentration range of 0.2-80.0 µg mL-1. The recovery% of MOL using the proposed method was 100.29%. The limit of detection (LOD) and limit of quantification (LOQ) were 0.04 µg mL-1 and 0.12 µg mL-1, respectively. No significant interference was detected in the presence of the common pharmaceutical formulation excipients. The method was validated following the ICH recommendations. All the obtained results were statistically compared with those using reported methods and there were no significant differences. The method developed in this work was successfully employed for the assessment of MOL in bulk powder and pharmaceutical formulation.

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Idioma: Inglês Revista: RSC Adv Ano de publicação: 2022 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Idioma: Inglês Revista: RSC Adv Ano de publicação: 2022 Tipo de documento: Artigo