GproDIA enables data-independent acquisition glycoproteomics with comprehensive statistical control.
Nat Commun
; 12(1): 6073, 2021 10 18.
Статья
в английский
| MEDLINE | ID: covidwho-1860369
ABSTRACT
Large-scale profiling of intact glycopeptides is critical but challenging in glycoproteomics. Data independent acquisition (DIA) is an emerging technology with deep proteome coverage and accurate quantitative capability in proteomics studies, but is still in the early stage of development in the field of glycoproteomics. We propose GproDIA, a framework for the proteome-wide characterization of intact glycopeptides from DIA data with comprehensive statistical control by a 2-dimentional false discovery rate approach and a glycoform inference algorithm, enabling accurate identification of intact glycopeptides using wide isolation windows. We further utilize a semi-empirical spectrum prediction strategy to expand the coverage of spectral libraries of glycopeptides. We benchmark our method for N-glycopeptide profiling on DIA data of yeast and human serum samples, demonstrating that DIA with GproDIA outperforms the data-dependent acquisition-based methods for glycoproteomics in terms of capacity and data completeness of identification, as well as accuracy and precision of quantification. We expect that this work can provide a powerful tool for glycoproteomic studies.
Полный текст:
Имеется в наличии
Коллекция:
Международные базы данных
база данных:
MEDLINE
Основная тема:
Glycopeptides
/
Proteome
/
Proteomics
Тип исследования:
Прогностическое исследование
Пределы темы:
Люди
Язык:
английский
Журнал:
Nat Commun
Тематика журнала:
Биология
/
Наука
Год:
2021
Тип:
Статья
Аффилированная страна:
S41467-021-26246-3
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