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Electric Cell-Substrate Impedance Sensing (ECIS) as a Platform for Evaluating Barrier-Function Susceptibility and Damage from Pulmonary Atelectrauma.
Yamaguchi, Eiichiro; Yao, Joshua; Aymond, Allison; Chrisey, Douglas B; Nieman, Gary F; Bates, Jason H T; Gaver, Donald P.
  • Yamaguchi E; Department of Biomedical Engineering, Tulane University, New Orleans, LA 70118, USA.
  • Yao J; Department of Biomedical Engineering, Tulane University, New Orleans, LA 70118, USA.
  • Aymond A; Department of Biomedical Engineering, Tulane University, New Orleans, LA 70118, USA.
  • Chrisey DB; Department of Physics and Engineering Physics, Tulane University, New Orleans, LA 70118, USA.
  • Nieman GF; Department of Surgery, Upstate Medical University, Syracuse, NY 13210, USA.
  • Bates JHT; Department of Medicine, University of Vermont, Burlington, VT 05405, USA.
  • Gaver DP; Department of Biomedical Engineering, Tulane University, New Orleans, LA 70118, USA.
Biosensors (Basel) ; 12(6)2022 Jun 05.
Статья в английский | MEDLINE | ID: covidwho-1884002
ABSTRACT
Biophysical insults that either reduce barrier function (COVID-19, smoke inhalation, aspiration, and inflammation) or increase mechanical stress (surfactant dysfunction) make the lung more susceptible to atelectrauma. We investigate the susceptibility and time-dependent disruption of barrier function associated with pulmonary atelectrauma of epithelial cells that occurs in acute respiratory distress syndrome (ARDS) and ventilator-induced lung injury (VILI). This in vitro study was performed using Electric Cell-substrate Impedance Sensing (ECIS) as a noninvasive evaluating technique for repetitive stress stimulus/response on monolayers of the human lung epithelial cell line NCI-H441. Atelectrauma was mimicked through recruitment/derecruitment (RD) of a semi-infinite air bubble to the fluid-occluded micro-channel. We show that a confluent monolayer with a high level of barrier function is nearly impervious to atelectrauma for hundreds of RD events. Nevertheless, barrier function is eventually diminished, and after a critical number of RD insults, the monolayer disintegrates exponentially. Confluent layers with lower initial barrier function are less resilient. These results indicate that the first line of defense from atelectrauma resides with intercellular binding. After disruption, the epithelial layer community protection is diminished and atelectrauma ensues. ECIS may provide a platform for identifying damaging stimuli, ventilation scenarios, or pharmaceuticals that can reduce susceptibility or enhance barrier-function recovery.
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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Respiratory Distress Syndrome / Pulmonary Atelectasis / Ventilator-Induced Lung Injury / COVID-19 Тип исследования: Экспериментальные исследования Темы: Длинный Ковид Пределы темы: Люди Язык: английский Год: 2022 Тип: Статья Аффилированная страна: Bios12060390

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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Respiratory Distress Syndrome / Pulmonary Atelectasis / Ventilator-Induced Lung Injury / COVID-19 Тип исследования: Экспериментальные исследования Темы: Длинный Ковид Пределы темы: Люди Язык: английский Год: 2022 Тип: Статья Аффилированная страна: Bios12060390