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Prospects of Coffee Leaf against SARS-CoV-2 Infection.
Wu, Chen-Shiou; Chiang, Hsiu-Mei; Chen, Yeh; Chen, Chung-Yu; Chen, Hsiao-Fan; Su, Wen-Chi; Wang, Wei-Jan; Chou, Yu-Chi; Chang, Wei-Chao; Wang, Shao-Chun; Hung, Mien-Chie.
  • Wu CS; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 406040, Taiwan.
  • Chiang HM; Department of Cosmeceutics, China Medical University, Taichung 406040, Taiwan.
  • Chen Y; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 406040, Taiwan.
  • Chen CY; Institute of New Drug Development, China Medical University, Taichung 406040, Taiwan.
  • Chen HF; Research Center for Cancer Biology, China Medical University, Taichung 406040, Taiwan.
  • Su WC; Research Center for Cancer Biology, China Medical University, Taichung 406040, Taiwan.
  • Wang WJ; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 406040, Taiwan.
  • Chou YC; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 406040, Taiwan.
  • Chang WC; International Master's Program of Biomedical Sciences, China Medical University, Taichung 406040, Taiwan.
  • Wang SC; Department of Medical Research, China Medical University Hospital, Taichung 404332, Taiwan.
  • Hung MC; Research Center for Cancer Biology, China Medical University, Taichung 406040, Taiwan.
Int J Biol Sci ; 18(12): 4677-4689, 2022.
Статья в английский | MEDLINE | ID: covidwho-1954700
ABSTRACT
In the current climate, many countries are in dire need of effective preventive methods to curb the Severe Acute Respiratory Syndrome Coronavirus Type 2 (SARS-CoV-2) pandemic. The purpose of this research is to screen and explore natural plant extracts that have the potential to against SARS-CoV-2 and provide alternative options for SARS-CoV-2 prevention and hand sanitizer or spray-like disinfectants. We first used Spike-ACE2 ELISA and TMPRSS2 fluorescence resonance energy transfer (FRET) assays to screen extracts from agricultural by-products from Taiwan with the potential to impede SARS-CoV-2 infection. Next, the SARS-CoV-2 pseudo-particles (Vpp) infection assay was tested to validate the effectiveness. We identified an extract from coffee leaf (Coffea Arabica), a natural plant that effectively inhibited wild-type SARS-CoV-2, and five Variants of Concern (Alpha, Beta, Gamma, Delta, and Omicron strain) from entering host cells. In an attempt to apply coffee leaf extract for hand sanitizer or spray-like disinfectants, we designed a skin-like gelatin membrane experiment. We showed that the high concentration of coffee leaf extract on the skin surface could block SARS-CoV-2 into cells more potently than 75% Ethanol, a standard disinfectant to inactivate SARS-CoV-2. Finally, LC-HRMS analysis was used to identify compounds such as caffeine, chlorogenic acid (CGA), quinic acid, and mangiferin that are associated with an anti-SARS-CoV-2 activity. Our results demonstrated that coffee leaf extract, an agricultural by-product effectively inhibits SARS-CoV-2 Vpp infection through an ACE2-dependent mechanism and may be utilized to develop products against SARS-CoV-2 infection.
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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Plant Extracts / Coffea / Hand Sanitizers / COVID-19 Тип исследования: Прогностическое исследование Темы: Варианты Язык: английский Журнал: Int J Biol Sci Тематика журнала: Биология Год: 2022 Тип: Статья Аффилированная страна: Ijbs.76058

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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Plant Extracts / Coffea / Hand Sanitizers / COVID-19 Тип исследования: Прогностическое исследование Темы: Варианты Язык: английский Журнал: Int J Biol Sci Тематика журнала: Биология Год: 2022 Тип: Статья Аффилированная страна: Ijbs.76058