An intra-cytoplasmic route for SARS-CoV-2 transmission unveiled by Helium-ion microscopy.
Sci Rep
; 12(1): 3794, 2022 03 08.
Статья
в английский
| MEDLINE | ID: covidwho-2004784
ABSTRACT
SARS-CoV-2 virions enter the host cells by docking their spike glycoproteins to the membrane-bound Angiotensin Converting Enzyme 2. After intracellular assembly, the newly formed virions are released from the infected cells to propagate the infection, using the extra-cytoplasmic ACE2 docking mechanism. However, the molecular events underpinning SARS-CoV-2 transmission between host cells are not fully understood. Here, we report the findings of a scanning Helium-ion microscopy study performed on Vero E6 cells infected with mNeonGreen-expressing SARS-CoV-2. Our data reveal, with unprecedented resolution, the presence of (1) long tunneling nanotubes that connect two or more host cells over submillimeter distances; (2) large scale multiple cell fusion events (syncytia); and (3) abundant extracellular vesicles of various sizes. Taken together, these ultrastructural features describe a novel intra-cytoplasmic connection among SARS-CoV-2 infected cells that may act as an alternative route of viral transmission, disengaged from the well-known extra-cytoplasmic ACE2 docking mechanism. Such route may explain the elusiveness of SARS-CoV-2 to survive from the immune surveillance of the infected host.
Полный текст:
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Коллекция:
Международные базы данных
база данных:
MEDLINE
Основная тема:
Virus Internalization
/
SARS-CoV-2
/
Microscopy
Тип исследования:
Прогностическое исследование
Пределы темы:
Животные
/
Люди
Язык:
английский
Журнал:
Sci Rep
Год:
2022
Тип:
Статья
Аффилированная страна:
S41598-022-07867-0
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