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Hesperetin from Root Extract of Clerodendrum petasites S. Moore Inhibits SARS-CoV-2 Spike Protein S1 Subunit-Induced NLRP3 Inflammasome in A549 Lung Cells via Modulation of the Akt/MAPK/AP-1 Pathway.
Arjsri, Punnida; Srisawad, Kamonwan; Mapoung, Sariya; Semmarath, Warathit; Thippraphan, Pilaiporn; Umsumarng, Sonthaya; Yodkeeree, Supachai; Dejkriengkraikul, Pornngarm.
  • Arjsri P; Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Srisawad K; Center for Research and Development of Natural Products for Health, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Mapoung S; Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Semmarath W; Anticarcinogenesis and Apoptosis Research Cluster, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Thippraphan P; Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Umsumarng S; Center for Research and Development of Natural Products for Health, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Yodkeeree S; Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Dejkriengkraikul P; Center for Research and Development of Natural Products for Health, Chiang Mai University, Chiang Mai 50200, Thailand.
Int J Mol Sci ; 23(18)2022 Sep 07.
Статья в английский | MEDLINE | ID: covidwho-2010122
ABSTRACT
Inhibition of inflammatory responses from the spike glycoprotein of SARS-CoV-2 (Spike) by targeting NLRP3 inflammasome has recently been developed as an alternative form of supportive therapy besides the traditional anti-viral approaches. Clerodendrum petasites S. Moore (C. petasites) is a Thai traditional medicinal plant possessing antipyretic and anti-inflammatory activities. In this study, C. petasites ethanolic root extract (CpEE) underwent solvent-partitioned extraction to obtain the ethyl acetate fraction of C. petasites (CpEA). Subsequently, C. petasites extracts were determined for the flavonoid contents and anti-inflammatory properties against spike induction in the A549 lung cells. According to the HPLC results, CpEA significantly contained higher amounts of hesperidin and hesperetin flavonoids than CpEE (p < 0.05). A549 cells were then pre-treated with either C. petasites extracts or its active flavonoids and were primed with 100 ng/mL of spike S1 subunit (Spike S1) and determined for the anti-inflammatory properties. The results indicate that CpEA (compared with CpEE) and hesperetin (compared with hesperidin) exhibited greater anti-inflammatory properties upon Spike S1 induction through a significant reduction in IL-6, IL-1ß, and IL-18 cytokine releases in A549 cells culture supernatant (p < 0.05). Additionally, CpEA and hesperetin significantly inhibited the Spike S1-induced inflammatory gene expressions (NLRP3, IL-1ß, and IL-18, p < 0.05). Mechanistically, CpEA and hesperetin attenuated inflammasome machinery protein expressions (NLRP3, ASC, and Caspase-1), as well as inactivated the Akt/MAPK/AP-1 pathway. Overall, our findings could provide scientific-based evidence to support the use of C. petasites and hesperetin in the development of supportive therapies for the prevention of COVID-19-related chronic inflammation.
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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Petasites / Clerodendrum / Antipyretics / COVID-19 Drug Treatment / Hesperidin Пределы темы: Люди Язык: английский Год: 2022 Тип: Статья Аффилированная страна: Ijms231810346

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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Petasites / Clerodendrum / Antipyretics / COVID-19 Drug Treatment / Hesperidin Пределы темы: Люди Язык: английский Год: 2022 Тип: Статья Аффилированная страна: Ijms231810346