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Precision-cut lung slices: A powerful ex vivo model to investigate respiratory infectious diseases.
Viana, Flávia; O'Kane, Cecilia M; Schroeder, Gunnar N.
  • Viana F; Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK.
  • O'Kane CM; Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK.
  • Schroeder GN; Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK.
Mol Microbiol ; 117(3): 578-588, 2022 03.
Статья в английский | MEDLINE | ID: covidwho-2063875
ABSTRACT
Respiratory infections are a leading cause of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid, or surrogate animal models. Although these provide important insights, they have limitations. Cell culture models fail to recapitulate cellular interactions in the lung and animal models often do not permit high-throughput analysis of drugs or pathogen isolates; hence, there is a need for improved, scalable models. Precision-cut lung slices (PCLS), small, uniform tissue slices generated from animal or human lungs are increasingly recognized and employed as an ex vivo organotypic model. PCLS retain remarkable cellular complexity and the architecture of the lung, providing a platform to investigate respiratory pathogens in a near-native environment. Here, we review the generation and features of PCLS, their use to investigate the pathogenesis of viral and bacterial pathogens, and highlight their potential to advance respiratory infection research in the future.
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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Communicable Diseases / Lung Пределы темы: Животные Язык: английский Журнал: Mol Microbiol Тематика журнала: Молекулярная биология / Микробиология Год: 2022 Тип: Статья Аффилированная страна: Mmi.14817

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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Communicable Diseases / Lung Пределы темы: Животные Язык: английский Журнал: Mol Microbiol Тематика журнала: Молекулярная биология / Микробиология Год: 2022 Тип: Статья Аффилированная страна: Mmi.14817