Compartmental immunophenotyping in COVID-19 ARDS: A case series.
J Allergy Clin Immunol
; 147(1): 81-91, 2021 01.
Статья
в английский
| MEDLINE | ID: covidwho-2095538
ABSTRACT
BACKGROUND:
Severe immunopathology may drive the deleterious manifestations that are observed in the advanced stages of coronavirus disease 2019 (COVID-19) but are poorly understood.OBJECTIVE:
Our aim was to phenotype leukocyte subpopulations and the cytokine milieu in the lungs and blood of critically ill patients with COVID-19 acute respiratory distress syndrome (ARDS).METHODS:
We consecutively included patients less than 72 hours after intubation following informed consent from their next of kin. Bronchoalveolar lavage fluid was evaluated by microscopy; bronchoalveolar lavage fluid and blood were assessed by 10-color flow cytometry and a multiplex cytokine panel.RESULTS:
Four mechanically ventilated patients (aged 40-75 years) with moderate-to-severe COVID-19 ARDS were included. Immature neutrophils dominated in both blood and lungs, whereas CD4 and CD8 T-cell lymphopenia was observed in the 2 compartments. However, regulatory T cells and TH17 cells were found in higher fractions in the lung. Lung CD4 and CD8 T cells and macrophages expressed an even higher upregulation of activation markers than in blood. A wide range of cytokines were expressed at high levels both in the blood and in the lungs, most notably, IL-1RA, IL-6, IL-8, IP-10, and monocyte chemoattactant protein-1, consistent with hyperinflammation.CONCLUSION:
COVID-19 ARDS exhibits a distinct immunologic profile in the lungs, with a depleted and exhausted CD4 and CD8 T-cell population that resides within a heavily hyperinflammatory milieu.ключевые слова
Полный текст:
Имеется в наличии
Коллекция:
Международные базы данных
база данных:
MEDLINE
Основная тема:
Respiratory Distress Syndrome
/
CD8-Positive T-Lymphocytes
/
Th17 Cells
/
SARS-CoV-2
/
COVID-19
/
Lung
/
Lymphopenia
Тип исследования:
Экспериментальные исследования
/
Наблюдательное исследование
/
Рандомизированные контролируемые испытания
Пределы темы:
Взрослые
/
Пожилые
/
Женщины
/
Люди
/
Мужчины
/
Middle aged
Язык:
английский
Журнал:
J Allergy Clin Immunol
Год:
2021
Тип:
Статья
Аффилированная страна:
J.jaci.2020.09.009
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