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Choosing a cellular model to study SARS-CoV-2.
Pires De Souza, Gabriel Augusto; Le Bideau, Marion; Boschi, Céline; Wurtz, Nathalie; Colson, Philippe; Aherfi, Sarah; Devaux, Christian; La Scola, Bernard.
  • Pires De Souza GA; Microbes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique - Hôpitaux de Marseille (AP-HM), Aix-Marseille Université, Marseille, France.
  • Le Bideau M; Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Boschi C; Microbes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique - Hôpitaux de Marseille (AP-HM), Aix-Marseille Université, Marseille, France.
  • Wurtz N; Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Colson P; Microbes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique - Hôpitaux de Marseille (AP-HM), Aix-Marseille Université, Marseille, France.
  • Aherfi S; Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Devaux C; Microbes, Evolution, Phylogeny and Infection (MEPHI), UM63, Institut de Recherche pour le Développement (IRD), Assistance Publique - Hôpitaux de Marseille (AP-HM), Aix-Marseille Université, Marseille, France.
  • La Scola B; Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
Front Cell Infect Microbiol ; 12: 1003608, 2022.
Статья в английский | MEDLINE | ID: covidwho-2109735
ABSTRACT
As new pathogens emerge, new challenges must be faced. This is no different in infectious disease research, where identifying the best tools available in laboratories to conduct an investigation can, at least initially, be particularly complicated. However, in the context of an emerging virus, such as SARS-CoV-2, which was recently detected in China and has become a global threat to healthcare systems, developing models of infection and pathogenesis is urgently required. Cell-based approaches are crucial to understanding coronavirus infection biology, growth kinetics, and tropism. Usually, laboratory cell lines are the first line in experimental models to study viral pathogenicity and perform assays aimed at screening antiviral compounds which are efficient at blocking the replication of emerging viruses, saving time and resources, reducing the use of experimental animals. However, determining the ideal cell type can be challenging, especially when several researchers have to adapt their studies to specific requirements. This review strives to guide scientists who are venturing into studying SARS-CoV-2 and help them choose the right cellular models. It revisits basic concepts of virology and presents the currently available in vitro models, their advantages and disadvantages, and the known consequences of each choice.
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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: SARS-CoV-2 / COVID-19 Тип исследования: Диагностическое исследование Пределы темы: Животные Страна как тема: Азия Язык: английский Журнал: Front Cell Infect Microbiol Год: 2022 Тип: Статья Аффилированная страна: Fcimb.2022.1003608

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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: SARS-CoV-2 / COVID-19 Тип исследования: Диагностическое исследование Пределы темы: Животные Страна как тема: Азия Язык: английский Журнал: Front Cell Infect Microbiol Год: 2022 Тип: Статья Аффилированная страна: Fcimb.2022.1003608