Novel Lateral Flow-Based Assay for Simple and Visual Detection of SARS-CoV-2 Mutations.
Front Cell Infect Microbiol
; 12: 902914, 2022.
Статья
в английский
| MEDLINE | ID: covidwho-2154670
ABSTRACT
Identification of the main SARS-CoV-2 variants in real time is of interest to control the virus and to rapidly devise appropriate public health responses. The RT-qPCR is currently considered to be the reference method to screen SARS-CoV-2 mutations, but it has some limitations. The multiplexing capability is limited when the number of markers to detect increases. Moreover, the performance of this allele-specific method may be impacted in the presence of new mutations. Herein, we present a proof-of-concept study of a simple molecular assay to detect key SARS-CoV-2 mutations. The innovative features of the assay are the multiplex asymmetric one-step RT-PCR amplification covering different regions of SARS-CoV-2 S gene and the visual detection of mutations on a lateral flow DNA microarray. Three kits (Kit 1 N501Y, E484K; Kit 2 L452R, E484K/Q; Kit 3 K417N, L452R, E484K/Q/A) were developed to match recommendations for surveillance of SARS-CoV-2 variants between January and December 2021. The clinical performance was assessed using RNA extracts from 113 SARS-CoV-2-positive samples with cycle thresholds <30, and results demonstrated that our assay allows specific and sensitive detection of mutations, with a performance comparable to that of RT-qPCR. The VAR-CoV assay detected four SARS-CoV-2 targets and achieved specific and sensitive screening of spike mutations associated with the main variants of concern, with a performance comparable to that of RT-qPCR. With well-defined virus sequences, this assay can be rapidly adapted to other emerging mutations; it is a promising tool for variant surveillance.
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Полный текст:
Имеется в наличии
Коллекция:
Международные базы данных
база данных:
MEDLINE
Основная тема:
SARS-CoV-2
/
COVID-19
Тип исследования:
Диагностическое исследование
/
Прогностическое исследование
Темы:
Варианты
Пределы темы:
Люди
Язык:
английский
Журнал:
Front Cell Infect Microbiol
Год:
2022
Тип:
Статья
Аффилированная страна:
Fcimb.2022.902914
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