Your browser doesn't support javascript.
Photochemical Identification of Auxiliary Severe Acute Respiratory Syndrome Coronavirus 2 Host Entry Factors Using µMap.
Suzuki, Saori; Geri, Jacob B; Knutson, Steve D; Bell-Temin, Harris; Tamura, Tomokazu; Fernández, David F; Lovett, Gabrielle H; Till, Nicholas A; Heller, Brigitte L; Guo, Jinchao; MacMillan, David W C; Ploss, Alexander.
  • Suzuki S; Department of Molecular Biology, Princeton University, Princeton, New Jersey08544, United States.
  • Geri JB; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States.
  • Knutson SD; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States.
  • Bell-Temin H; Bristol Myers Squibb, Princeton, New Jersey08540, United States.
  • Tamura T; Department of Molecular Biology, Princeton University, Princeton, New Jersey08544, United States.
  • Fernández DF; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States.
  • Lovett GH; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States.
  • Till NA; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States.
  • Heller BL; Department of Molecular Biology, Princeton University, Princeton, New Jersey08544, United States.
  • Guo J; Department of Molecular Biology, Princeton University, Princeton, New Jersey08544, United States.
  • MacMillan DWC; Department of Chemistry, Princeton University, Princeton, New Jersey08544, United States.
  • Ploss A; Merck Center for Catalysis at Princeton University, Princeton, New Jersey08544, United States.
J Am Chem Soc ; 144(36): 16604-16611, 2022 09 14.
Статья в английский | MEDLINE | ID: covidwho-2185543
ABSTRACT
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the infectious agent of the COVID-19 pandemic, remains a global medical problem. Angiotensin-converting enzyme 2 (ACE2) was identified as the primary viral entry receptor, and transmembrane serine protease 2 primes the spike protein for membrane fusion. However, ACE2 expression is generally low and variable across tissues, suggesting that auxiliary receptors facilitate viral entry. Identifying these factors is critical for understanding SARS-Cov-2 pathophysiology and developing new countermeasures. However, profiling host-virus interactomes involves extensive genetic screening or complex computational predictions. Here, we leverage the photocatalytic proximity labeling platform µMap to rapidly profile the spike interactome in human cells and identify eight novel candidate receptors. We systemically validate their functionality in SARS-CoV-2 pseudoviral uptake assays with both Wuhan and Delta spike variants and show that dual expression of ACE2 with either neuropilin-2, ephrin receptor A7, solute carrier family 6 member 15, or myelin and lymphocyte protein 2 significantly enhances viral uptake. Collectively, our data show that SARS-CoV-2 synergistically engages several host factors for cell entry and establishes µMap as a powerful tool for rapidly interrogating host-virus interactomes.
Тема - темы

Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: SARS-CoV-2 / COVID-19 Тип исследования: Прогностическое исследование Темы: Варианты Пределы темы: Люди Язык: английский Журнал: J Am Chem Soc Год: 2022 Тип: Статья Аффилированная страна: Jacs.2c06806

Документы, близкие по теме

MEDLINE

...
LILACS

LIS


Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: SARS-CoV-2 / COVID-19 Тип исследования: Прогностическое исследование Темы: Варианты Пределы темы: Люди Язык: английский Журнал: J Am Chem Soc Год: 2022 Тип: Статья Аффилированная страна: Jacs.2c06806