Improved transfer efficiency of supercharged 36 + GFP protein mediate nucleic acid delivery.
Drug Deliv
; 29(1): 386-398, 2022 Dec.
Статья
в английский
| MEDLINE | ID: covidwho-2187330
ABSTRACT
The potential of nucleic acid therapeutics to treat diseases by targeting specific cells has resulted in its increasing number of uses in clinical settings. However, the major challenge is to deliver bio-macromolecules into target cells and/or subcellular locations of interest ahead in the development of delivery systems. Although, supercharged residues replaced protein 36 + GFP can facilitate itself and cargoes delivery, its efficiency is still limited. Therefore, we combined our recent progress to further improve 36 + GFP based delivery efficiency. We found that the penetration efficacy of 36 + GFP protein was significantly improved by fusion with CPP-Dot1l or treatment with penetration enhancer dimethyl sulfoxide (DMSO) in vitro. After safely packaged with plasmid DNA, we found that the efficacy of in vitro and in vivo transfection mediated by 36 + GFP-Dot1l fusion protein is also significantly improved than 36 + GFP itself. Our findings illustrated that fusion with CPP-Dot1l or incubation with DMSO is an alternative way to synergically promote 36 + GFP mediated plasmid DNA delivery in vitro and in vivo.
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Полный текст:
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Коллекция:
Международные базы данных
база данных:
MEDLINE
Основная тема:
Nucleic Acids
/
Histone-Lysine N-Methyltransferase
/
Drug Delivery Systems
/
Green Fluorescent Proteins
/
Cell-Penetrating Peptides
Тип исследования:
Прогностическое исследование
Пределы темы:
Животные
/
Люди
Язык:
английский
Журнал:
Drug Deliv
Тематика журнала:
Фармакология
/
Лекарственная терапия
Год:
2022
Тип:
Статья
Аффилированная страна:
10717544.2022.2030430
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