In Silico and In Vitro Inhibition of SARS-CoV-2 PLpro with Gramicidin D.
Int J Mol Sci
; 24(3)2023 Jan 19.
Статья
в английский
| MEDLINE | ID: covidwho-2244379
ABSTRACT
Finding an effective drug to prevent or treat COVID-19 is of utmost importance in tcurrent pandemic. Since developing a new treatment takes a significant amount of time, drug repurposing can be an effective option for achieving a rapid response. This study used a combined in silico virtual screening protocol for candidate SARS-CoV-2 PLpro inhibitors. The Drugbank database was searched first, using the Informational Spectrum Method for Small Molecules, followed by molecular docking. Gramicidin D was selected as a peptide drug, showing the best in silico interaction profile with PLpro. After the expression and purification of PLpro, gramicidin D was screened for protease inhibition in vitro and was found to be active against PLpro. The current study's findings are significant because it is critical to identify COVID-19 therapies that are efficient, affordable, and have a favorable safety profile.
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Полный текст:
Имеется в наличии
Коллекция:
Международные базы данных
база данных:
MEDLINE
Основная тема:
SARS-CoV-2
/
COVID-19
Тип исследования:
Guideline
/
Прогностическое исследование
Пределы темы:
Люди
Язык:
английский
Год:
2023
Тип:
Статья
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