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Differentially induced immunity in buccal and nasal mucosae after vaccination for SARS-CoV-2: Prospects for mass scale immunity-screening in large populations.
Tsamadou, Chrysanthi; Ludwig, Carolin; Scholz, Judith; Proffen, Matthias; Hägele, Janina; Rode, Immanuel; Körper, Sixten; Fabricius, Dorit; Jahrsdörfer, Bernd; Neuchel, Christine; Amann, Elisa; Schrezenmeier, Hubert; Fürst, Daniel.
  • Tsamadou C; Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service, University Hospital Ulm, Ulm, Baden-Wuerttemberg, Germany.
  • Ludwig C; Institute of Transfusion Medicine, University of Ulm, Ulm, Germany.
  • Scholz J; Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service, University Hospital Ulm, Ulm, Baden-Wuerttemberg, Germany.
  • Proffen M; Institute of Transfusion Medicine, University of Ulm, Ulm, Germany.
  • Hägele J; Institute of Transfusion Medicine, University of Ulm, Ulm, Germany.
  • Rode I; Institute of Transfusion Medicine, University of Ulm, Ulm, Germany.
  • Körper S; Institute of Transfusion Medicine, University of Ulm, Ulm, Germany.
  • Fabricius D; Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service, University Hospital Ulm, Ulm, Baden-Wuerttemberg, Germany.
  • Jahrsdörfer B; Institute of Transfusion Medicine, University of Ulm, Ulm, Germany.
  • Neuchel C; Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service, University Hospital Ulm, Ulm, Baden-Wuerttemberg, Germany.
  • Amann E; Institute of Transfusion Medicine, University of Ulm, Ulm, Germany.
  • Schrezenmeier H; Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, Ulm, Germany.
  • Fürst D; Institute of Clinical Transfusion Medicine and Immunogenetics Ulm, German Red Cross Blood Transfusion Service, University Hospital Ulm, Ulm, Baden-Wuerttemberg, Germany.
Front Immunol ; 13: 999693, 2022.
Статья в английский | MEDLINE | ID: covidwho-2326746
ABSTRACT

Introduction:

Humoral immunity after SARS-CoV-2 vaccination has been extensively investigated in blood. Aim of this study was to develop an ELISA method in order to determine the prevalence of IgG and IgA SARS-CoV-2 domain 1 spike-protein (S) specific antibodies (Abs) in buccal and nasal mucosal surfaces of vaccinees.

Methods:

To this end, we analyzed 69 individuals who received their first vaccine dose between February and July 2021. Vaccines administered were BNT162b2, mRNA-1273 or ChAdOx1-nCoV-19. Detection of IgG and IgA Abs was performed using commercial ELISA kits for both blood and swab samples after protocol modification for the latter.

Results:

Anti-spike IgG and IgA Abs in the buccal and/or nasal swabs were detectable in >81% of the study subjects after the second dose. The IgG measurements in buccal swabs appeared to correlate in a more consistent way with the respective measurements in blood with a correlation coefficient of r=0.74. It is of note that IgA Abs appeared to be significantly more prevalent in the nasal compared to the buccal mucosa. Optimal selection of the assay cut-off for the IgG antibody detection in buccal swabs conferred a sensitivity of 91.8% and a specificity of 100%. Last, individuals vaccinated with mRNA-based vaccines exhibited higher antibody levels in both blood and mucosal surfaces compared to those receiving ChAdOx1-nCoV-19 confirming previously reported results.

Conclusion:

In conclusion, our findings show a differential prevalence of anti-S Abs on mucosal surfaces after vaccination for SARS-CoV-2, while they also set the basis for potential future use of IgG antibody detection in buccal swabs for extended immunity screening in large populations.
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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: SARS-CoV-2 / COVID-19 Тип исследования: Диагностическое исследование / Наблюдательное исследование Темы: Вакцина / Варианты Пределы темы: Люди Язык: английский Журнал: Front Immunol Год: 2022 Тип: Статья Аффилированная страна: Fimmu.2022.999693

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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: SARS-CoV-2 / COVID-19 Тип исследования: Диагностическое исследование / Наблюдательное исследование Темы: Вакцина / Варианты Пределы темы: Люди Язык: английский Журнал: Front Immunol Год: 2022 Тип: Статья Аффилированная страна: Fimmu.2022.999693