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Epithelial-mesenchymal transition of absorptive enterocytes and depletion of Peyer's patch M cells after PEDV infection.
Chen, Ya-Mei; Helm, Emma T; Groeltz-Thrush, Jennifer M; Gabler, Nicholas K; Burrough, Eric R.
  • Chen YM; Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
  • Helm ET; Department of Animal Science, Iowa State University, College of Agriculture and Life Sciences, Ames, IA, USA.
  • Groeltz-Thrush JM; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.
  • Gabler NK; Department of Animal Science, Iowa State University, College of Agriculture and Life Sciences, Ames, IA, USA.
  • Burrough ER; Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA. Electronic address: burrough@iastate.edu.
Virology ; 552: 43-51, 2021 01 02.
Статья в английский | MEDLINE | ID: covidwho-843443
ABSTRACT
This study focused on intestinal restitution including phenotype switching of absorptive enterocytes and the abundance of different enterocyte subtypes in weaned pigs after porcine epidemic diarrhea virus (PEDV) infection. At 10 days post-PEDV-inoculation, the ratio of villus height to crypt depth in both jejunum and ileum had restored, and the PEDV antigen was not detectable. However, enterocytes at the villus tips revealed epithelial-mesenchymal transition (EMT) in the jejunum in which E-cadherin expression decreased while expression of N-cadherin, vimentin, and Snail increased. Additionally, there was reduced expression of actin in microvilli and Zonula occludens-1 (ZO-1) in tight junctions. Moreover, the protein concentration of transforming growth factor ß1 (TGFß1), which mediates EMT and cytoskeleton alteration, was increased. We also found a decreased number of Peyer's patch M cells in the ileum. These results reveal incomplete restitution of enterocytes in the jejunum and potentially impaired immune surveillance in the ileum after PEDV infection.
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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Peyer's Patches / Coronavirus Infections / Enterocytes / Porcine epidemic diarrhea virus / Epithelial-Mesenchymal Transition / Gastroenteritis, Transmissible, of Swine Пределы темы: Животные Язык: английский Журнал: Virology Год: 2021 Тип: Статья Аффилированная страна: J.virol.2020.08.018

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Полный текст: Имеется в наличии Коллекция: Международные базы данных база данных: MEDLINE Основная тема: Peyer's Patches / Coronavirus Infections / Enterocytes / Porcine epidemic diarrhea virus / Epithelial-Mesenchymal Transition / Gastroenteritis, Transmissible, of Swine Пределы темы: Животные Язык: английский Журнал: Virology Год: 2021 Тип: Статья Аффилированная страна: J.virol.2020.08.018