Performance verification of anti-SARS-CoV-2-specific antibody detection by using four chemiluminescence immunoassay systems.
Ann Clin Biochem
; 57(6): 429-434, 2020 11.
Статья
в английский
| MEDLINE | ID: covidwho-982712
ABSTRACT
OBJECTIVES:
The purpose of the current study was to evaluate the analytical performance of seven kits for detecting IgM/IgG antibodies against coronavirus (SARS-CoV-2) by using four chemiluminescence immunoassay systems.METHODS:
Fifty patients diagnosed with SARS-CoV-2 infection and 130 controls without coronavirus infection from the General Hospital of Chongqing were enrolled in the current retrospective study. Four chemiluminescence immunoassay systems, including seven IgM/IgG antibody detection kits for SARS-CoV-2 (A_IgM, A_IgG, B_IgM, B_IgG, C_IgM, C_IgG and D_Ab), were employed to detect antibody concentrations. The chi-square test, the receiver operating characteristic (ROC) curve and Youden's index were determined to verify the cut-off value of each detection system.RESULTS:
The repeatability verification results of the A, B, C and D systems are all qualified. D_Ab performed best (92% sensitivity and 99.23% specificity), and B_IgM performed worse than the other systems. Except for the A_IgM and C_IgG systems, the optimal diagnostic thresholds and cut-off values of the other kits and their recommendations are inconsistent with each other. B_IgM had the worst AUC, and C_IgG had the best diagnostic accuracy. More importantly, the B_IgG system had the highest false-positive rate for testing patients with AIDS, tumours and pregnancies. The A_IgM system test showed the highest false-positive rates among elderly individuals over 90 years old. COVID-2019 IgM/IgG antibody test systems exhibit performance differences.CONCLUSIONS:
The Innodx Biotech Total Antibody serum diagnosis kit is the most reliable detection system for anti-SARS-CoV-2 antibodies, which can be used together with nucleic acid tests as an alternative method for SARS-CoV-2 detecting.ключевые слова
Полный текст:
Имеется в наличии
Коллекция:
Международные базы данных
база данных:
MEDLINE
Основная тема:
Immunoassay
/
Luminescence
/
Betacoronavirus
/
Antibodies, Viral
/
Antibody Specificity
Тип исследования:
Диагностическое исследование
/
Экспериментальные исследования
/
Наблюдательное исследование
Пределы темы:
Женщины
/
Люди
/
Мужчины
/
Middle aged
Язык:
английский
Журнал:
Ann Clin Biochem
Год:
2020
Тип:
Статья
Аффилированная страна:
0004563220963847
Документы, близкие по теме
MEDLINE
...
LILACS
LIS