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Pesquisa | Influenza A (H1N1)

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Resultados  1-12 de 118

Evaluation of twenty-two rapid antigen detection tests in the diagnosis of Equine Influenza caused by viruses of H3N8 subtype.

Autor(es): Yamanaka, Takashi; Nemoto, Manabu; Bannai, Hiroshi; Tsujimura, Koji; Kondo, Takashi; Matsumura, Tomio; Gildea, Sarah; Cullinane, Ann
Fonte: Influenza Other Respir Viruses;10(2): 127-33, 2016 Mar.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26568369
Resumo: BACKGROUND: Equine influenza (EI) is a highly contagious disease caused by viruses of the H3N8 subtype. The rapid diagnosis of EI is essential to reduce the disease spread. Many rapid antigen detection (RAD) tests for diagnosing human influenza are available, but their ability to diagnose EI has not been systematically evaluated. OBJECTIVES: The aim of this study was to compare the performance of 22 RAD tests in the diagnosis of EI. METHODS: The 22 RAD tests were performed on fivefold seria (mais)

The Role of [ F]FDG-PET/CT in Predicting Malignant Transformation of Plexiform Neurofibromas in Neurofibromatosis-1.

Autor(es): Tovmassian, David; Abdul Razak, Muzib; London, Kevin
Fonte: Int J Surg Oncol;2016: 6162182, 2016.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 28058117
Resumo: . Malignant peripheral nerve sheath tumours (MPNSTs) are difficult to diagnose and treat and contribute to significant morbidity and mortality for patients with Neurofibromatosis-1 (NF-1). FDG-PET/CT is being increasingly used as an imaging modality to discriminate between benign and malignant plexiform neurofibromas. . To assess the value of FDG-PET/CT in differentiating between benign and malignant peripheral nerve lesions for patients with Neurofibromatosis-1. . A systematic review of (mais)


Autor(es): Ippolitov, E V; Didenko, L V; Tzarev, V N
Fonte: Klin Lab Diagn;60(12): 59-64, 2015 Dec.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27032256
Resumo: The study was carried out to analyze morphology of biofilm of periodontium and to develop electronic microscopic criteria of differentiated diagnostic of inflammatory diseases of gums. The scanning electronic microscopy was applied to analyze samples of bioflm of periodont from 70 patients. Including ten patients with every nosologic form of groups with chronic catarrhal periodontitis. of light, mean and severe degree, chronic catarrhal gingivitis, Candida-associated paroperiodontitis and 2 (mais)

A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.

Autor(es): Miarka, Maciej; Horban, Andrzej; Maliszewska, Henryka; Bilinski, Przemyslaw; Prus-Kowalczuk, Wanda
Fonte: Acta Biochim Pol;61(3): 485-7, 2014.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 25210936
Resumo: INTRODUCTION AND OBJECTIVE: In June 2009 the World Health Organization announced influenza pandemic caused by A/H1N1/v virus. It became crucial to recognize new cases of A/H1N1/v infection. An effective screening diagnostic procedure was needed for patients suffering from influenza-like symptoms for making an initial diagnosis and analyzing epidemiological pattern of infection. We used a strip test for influenza A/B as a screening diagnostic procedure for patients suffering from influenza-l (mais)

[Evaluation of the sensitivity of a densitometry system, in judging the result of influenza virus antigen-detection kit using immunochromatography].

Autor(es): Yamaguchi, Ikuo; Aoyama, Tomoe; Yamamoto, Masaru; Kinosita, Keiko; Ito, Yumi
Fonte: Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi;24(2): 69-70, 2014.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 25073199
Resumo: Immunochromatography viral antigen-detection kits have become popular in clinical settings in Japan. Influenza virus detection kit is one of them. It is sometimes used in early phase of the disease, combined with the early treatment with anti-influenza drugs. Most of them are invented to visually read the test line on their kits. However, we should be careful about their reliability of them. Sometimes human errors occur at the visual tests, and they have different sensitivities among the ki (mais)

Microfluidic in-reservoir pre-concentration using a buffer drain technique.

Autor(es): Yoon, Junghyo; Cho, Youngkyu; Han, Sewoon; Lim, Chae Seung; Lee, Jeong Hoon; Chung, Seok
Fonte: Lab Chip;14(15): 2778-82, 2014 Aug 07.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 24905601
Resumo: Pre-concentration methods are essential for detecting low concentrations of influenza virus in biological samples from patients. Here, we describe a new method for draining buffer from solution in the reservoir of a microfluidic device to increase the concentration of virus in the reservoir. Viruses were captured in the reservoir by an ion depletion barrier from connected ion selective microfluidic channels. 75 µl of buffer was successfully drained from a 100 µl sample, resulting in a 4-f (mais)

Point-of-care multiplexed assays of nucleic acids using microcapillary-based loop-mediated isothermal amplification.

Autor(es): Zhang, Yi; Zhang, Lu; Sun, Jiashu; Liu, Yulei; Ma, Xingjie; Cui, Shangjin; Ma, Liying; Xi, Jianzhong Jeff; Jiang, Xingyu
Fonte: Anal Chem;86(14): 7057-62, 2014 Jul 15.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 24937125
Resumo: This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. This assay integrates capillaries (glass or plastic) to introduce and house sample/reagents, segments of water droplets to prevent contamination, pocket warmers to provide heat, and a hand-held flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA ta (mais)

Contribution of systematic RT-PCR screening for influenza during the epidemic season.

Autor(es): Martinot, M; Heller, R; Martin, A; Sagot, E; Souply, L; Mothes, A; Mohseni-Zadeh, M; de Briel, D
Fonte: Med Mal Infect;44(3): 123-7, 2014 Mar.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 24612505
Resumo: OBJECTIVE: We assessed the systematic RT-PCR screening of patients admitted to an infectious diseases department (IDD), during the 2012-2013 influenza outbreak. METHODOLOGY: Patients admitted with cough and fever underwent a nasopharyngeal smear for RT-PCR screening. RESULTS: Ninety-eight patients were admitted in the IDD, from January 1st to February 22nd, 46 were screened; 11 male and 6 female patients (17.3%, mean age of 68 years) were positive. The diagnoses made in the emergency depart (mais)

The evaluation of three diagnostic tests for the detection of equine influenza nucleoprotein in nasal swabs.

Autor(es): Galvin, Pamela; Gildea, Sarah; Nelly, Maura; Quinlivan, Michelle; Arkins, Sean; Walsh, Cathal; Cullinane, Ann
Fonte: Influenza Other Respir Viruses;8(3): 376-83, 2014 May.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 24512560
Resumo: BACKGROUND: Equine influenza (EI) is a highly contagious respiratory disease of horses. OBJECTIVES: The aim of this study was to evaluate two rapid antigen detection kits (Directigen or DFA, and Espline) and a commercial ELISA for the detection of EI nucleoprotein in nasal swabs. METHOD: Nasal swab samples from naturally and experimentally infected horses were used to compare the sensitivity and specificity of these assays to virus isolation (VI) and real-time RT-PCR. RESULTS: If real-time (mais)

Simultaneous detection of influenza viruses A, B, and swine origin influenza A using multiplex one-step real-time RT-PCR assay.

Autor(es): Monavari, S H R; Mollaie, H R; Fazlalipour, M
Fonte: Appl Biochem Biotechnol;172(2): 984-92, 2014 Jan.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 24142356
Resumo: Every year, seasonal epidemics of influenza viruses are causing considerable morbidity and mortality worldwide. Also infrequent novel and rearranged strains of influenza viruses have caused quick, acute universal pandemics resulting in millions of mortalities. The usage of efficient and accurate detection is superior for infection control, effective treatment, and epidemiological supervision. Therefore, evaluation of useful real-time PCR molecular tests for the detection of pandemic viruses (mais)

Evolving gene targets and technology in influenza detection.

Autor(es): Malanoski, Anthony P; Lin, Baochuan
Fonte: Mol Diagn Ther;17(5): 273-86, 2013 Oct.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 23686537
Resumo: Influenza viruses cause recurring epidemic outbreaks every year associated with high morbidity and mortality. Despite extensive research and surveillance efforts to control influenza outbreaks, the primary mitigation treatment for influenza is the development of yearly vaccine mixes targeted for the most prevalent virus strains. Consequently, the focus of many detection technologies has evolved toward accurate identification of subtype and understanding the evolution and molecular determina (mais)

Evaluation of Wondfo influenza A&B fast test based on immunochromatography assay for rapid diagnosis of influenza A H1N1.

Autor(es): Peng, Yunping; Wu, Junlin; Liu, Xiaoyun; Wang, Jihua; Li, Wenmei
Fonte: Braz J Infect Dis;17(2): 247-50, 2013 Mar-Apr.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 23465599
Resumo: Influenza viruses cause significant morbidity and mortality in both children and adults during local outbreaks or epidemics. Therefore, a rapid test for influenza A&B would be useful. This study was conducted to evaluate the clinical performance of the Wondfo influenza A&B test for rapid diagnosis of influenza A H1N1 Infection. The rapid testing assay could distinguish infection of influenza A and B virus. The reference viral strains were cultured in MDCK cells while TCID50 if the viruses w (mais)
Resultados  1-12 de 118