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Pesquisa | Influenza A (H1N1)

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Resultados  1-12 de 122
1.

Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing/ Optimización de la detección de SARS-CoV-2 mediante el análisis de muestras agrupadas

Autor(es): Farfan, Mauricio J; Torres, Juan P; ORyan, Miguel; Olivares, Mauricio; Gallardo, Pablo; Lastra, Jorge; Salas, Carolina
Fonte: Rev. chil. infectol;37(3): 276-280, jun. 2020. tab, graf
LILACS - Literatura Latino-Americana e do Caribe em Ciências da Saúde ID: 1126120
Resumo: Abstract The global shortage of reagents and kits for nucleic acid extraction and molecular detection of SARS-CoV-2 requires new cost-effective strategies for the diagnosis of suspected COVID-19 cases, especially in countries that need to increase detection capacity. Pooled nucleic acid testing has been extensively used as a cost-effective strategy for HIV, HepB, HepC and influenza. Also, protocols dispensing of RNA extraction appears as an attractive option for detection of SARS-CoV-2. In (mais)
2.

Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus.

Autor(es): Ozkan, Busra; Budama-Kilinc, Yasemin; Cakir-Koc, Rabia; Mese, Sevim; Badur, Selim
Fonte: Bioengineered;10(1): 33-42, 2019 12.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 30913952
Resumo: The diagnosis of influenza A virus is essential since it can be confused with influenza A like illness and lead to inaccurate drug prescription. In this study, the M2e peptide, a strategic antigen that is conserved in all virus subtypes, was used as a diagnostic marker of influenza A. For the first time, M2e-specific IgY antibody was covalently conjugated to alkaline phosphatase (ALP) enzyme in the presence of glutaraldehyde. The antibody-enzyme bioconjugate was characterized by fluorescenc (mais)
3.

Rapid Antigen Tests for Influenza: Rationale and Significance of the FDA Reclassification.

Autor(es): Green, Daniel A; StGeorge, Kirsten
Fonte: J Clin Microbiol;56(10)2018 10.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 29899007
Resumo: Rapid antigen tests for influenza, here referred to as rapid influenza diagnostic tests (RIDTs), have been widely used for the diagnosis of influenza since their introduction in the 1990s due to their ease of use, rapid results, and suitability for point of care (POC) testing. However, issues related to the diagnostic sensitivity of these assays have been known for decades, and these issues gained greater attention following reports of their poor performance during the 2009 influenza A(H1N1 (mais)
4.

The Drift in Molecular Testing for Influenza: Mutations Affecting Assay Performance.

Autor(es): Stellrecht, Kathleen A
Fonte: J Clin Microbiol;56(3)2018 03.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 29305549
Resumo: Influenza is associated with rapid evolution due to lack of RNA polymerase proofreading, immunogenic selection, and frequent rearrangement of gene segments. Evolutionary changes affecting the performance of diagnostic testing have long been recognized. Hence, it is not surprising that such challenges apply to nucleic acid amplification tests, even though they are designed to target highly conserved regions. Initially, case reports involved single isolates of A(H1N1)pdm09. Over the past 4 ye (mais)
5.

Fluorescent Neuraminidase Assay Based on Supramolecular Dye Capture After Enzymatic Cleavage.

Autor(es): Liu, Wenqi; Gómez-Durán, César F A; Smith, Bradley D
Fonte: J Am Chem Soc;139(18): 6390-6395, 2017 05 10.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 28426220
Resumo: A conceptually new type of enzymatic cleavage assay is reported that utilizes in situ supramolecular capture of the fluorescent product. A squaraine-derived substrate with large blocking groups at each end of its structure cannot be threaded by a tetralactam macrocycle until the blocking groups are removed by enzyme cleavage. A prototype design responds to viral neuraminidase, an indicator of influenza infection, and also measures susceptibility of the sample to neuraminidase inhibitor drug (mais)
6.

Rapid Identification of the Receptor-Binding Specificity of Influenza A Viruses by Fluorogenic Glycofoldamers.

Autor(es): He, Xiao-Peng; Zeng, Ya-Li; Tang, Xin-Ying; Li, Na; Zhou, Dong-Ming; Chen, Guo-Rong; Tian, He
Fonte: Angew Chem Int Ed Engl;55(45): 13995-13999, 2016 11 02.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27513450
Resumo: The re-emergence of influenza raises a global concern that viral pandemics can unpredictably occur. However, effective approaches that can probe the infection risk of influenza viruses for humans are rare. In this work, we develop a glycofoldamer that can rapidly identify the glycan-receptor specificity of influenza viruses in a high-throughput manner. The coupling of glycan receptors that can be recognized by hemagglutinin (a surface protein on the virion capsid of influenza) to a fluoroge (mais)
7.

Evaluation of twenty-two rapid antigen detection tests in the diagnosis of Equine Influenza caused by viruses of H3N8 subtype.

Autor(es): Yamanaka, Takashi; Nemoto, Manabu; Bannai, Hiroshi; Tsujimura, Koji; Kondo, Takashi; Matsumura, Tomio; Gildea, Sarah; Cullinane, Ann
Fonte: Influenza Other Respir Viruses;10(2): 127-33, 2016 Mar.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26568369
Resumo: BACKGROUND: Equine influenza (EI) is a highly contagious disease caused by viruses of the H3N8 subtype. The rapid diagnosis of EI is essential to reduce the disease spread. Many rapid antigen detection (RAD) tests for diagnosing human influenza are available, but their ability to diagnose EI has not been systematically evaluated. OBJECTIVES: The aim of this study was to compare the performance of 22 RAD tests in the diagnosis of EI. METHODS: The 22 RAD tests were performed on fivefold seria (mais)
8.

A clinical utility of a strip test for influenza A/B and comparison with detection by RT PCR.

Autor(es): Miarka, Maciej; Horban, Andrzej; Maliszewska, Henryka; Bilinski, Przemyslaw; Prus-Kowalczuk, Wanda
Fonte: Acta Biochim Pol;61(3): 485-7, 2014.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 25210936
Resumo: INTRODUCTION AND OBJECTIVE: In June 2009 the World Health Organization announced influenza pandemic caused by A/H1N1/v virus. It became crucial to recognize new cases of A/H1N1/v infection. An effective screening diagnostic procedure was needed for patients suffering from influenza-like symptoms for making an initial diagnosis and analyzing epidemiological pattern of infection. We used a strip test for influenza A/B as a screening diagnostic procedure for patients suffering from influenza-l (mais)
9.

[Evaluation of the sensitivity of a densitometry system, in judging the result of influenza virus antigen-detection kit using immunochromatography].

Autor(es): Yamaguchi, Ikuo; Aoyama, Tomoe; Yamamoto, Masaru; Kinosita, Keiko; Ito, Yumi
Fonte: Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi;24(2): 69-70, 2014.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 25073199
Resumo: Immunochromatography viral antigen-detection kits have become popular in clinical settings in Japan. Influenza virus detection kit is one of them. It is sometimes used in early phase of the disease, combined with the early treatment with anti-influenza drugs. Most of them are invented to visually read the test line on their kits. However, we should be careful about their reliability of them. Sometimes human errors occur at the visual tests, and they have different sensitivities among the ki (mais)
10.

Point-of-care multiplexed assays of nucleic acids using microcapillary-based loop-mediated isothermal amplification.

Autor(es): Zhang, Yi; Zhang, Lu; Sun, Jiashu; Liu, Yulei; Ma, Xingjie; Cui, Shangjin; Ma, Liying; Xi, Jianzhong Jeff; Jiang, Xingyu
Fonte: Anal Chem;86(14): 7057-62, 2014 Jul 15.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 24937125
Resumo: This report demonstrates a straightforward, robust, multiplexed and point-of-care microcapillary-based loop-mediated isothermal amplification (cLAMP) for assaying nucleic acids. This assay integrates capillaries (glass or plastic) to introduce and house sample/reagents, segments of water droplets to prevent contamination, pocket warmers to provide heat, and a hand-held flashlight for a visual readout of the fluorescent signal. The cLAMP system allows the simultaneous detection of two RNA ta (mais)
11.

Microfluidic in-reservoir pre-concentration using a buffer drain technique.

Autor(es): Yoon, Junghyo; Cho, Youngkyu; Han, Sewoon; Lim, Chae Seung; Lee, Jeong Hoon; Chung, Seok
Fonte: Lab Chip;14(15): 2778-82, 2014 Aug 07.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 24905601
Resumo: Pre-concentration methods are essential for detecting low concentrations of influenza virus in biological samples from patients. Here, we describe a new method for draining buffer from solution in the reservoir of a microfluidic device to increase the concentration of virus in the reservoir. Viruses were captured in the reservoir by an ion depletion barrier from connected ion selective microfluidic channels. 75 µl of buffer was successfully drained from a 100 µl sample, resulting in a 4-f (mais)
12.

Contribution of systematic RT-PCR screening for influenza during the epidemic season.

Autor(es): Martinot, M; Heller, R; Martin, A; Sagot, E; Souply, L; Mothes, A; Mohseni-Zadeh, M; de Briel, D
Fonte: Med Mal Infect;44(3): 123-7, 2014 Mar.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 24612505
Resumo: OBJECTIVE: We assessed the systematic RT-PCR screening of patients admitted to an infectious diseases department (IDD), during the 2012-2013 influenza outbreak. METHODOLOGY: Patients admitted with cough and fever underwent a nasopharyngeal smear for RT-PCR screening. RESULTS: Ninety-eight patients were admitted in the IDD, from January 1st to February 22nd, 46 were screened; 11 male and 6 female patients (17.3%, mean age of 68 years) were positive. The diagnoses made in the emergency depart (mais)
Resultados  1-12 de 122