Biblioteca Virtual em Saúde

Pesquisa | Influenza A (H1N1)

  • BIREME | OPAS | OMS logo
 

Histórico de pesquisa  ()

Sua seleção  ()

 0 documento(s) selecionado(s).

Refine sua pesquisa

Collapse All  Ocultar
Expand All  Mostrar
Tipo
Áreas temáticas
Texto completo (392)
Idioma
Ano de publicação

Enviar por email

Você tem 0 documento(s) selecionado(s).

Nenhum documento selecionado

 
Resultados  1-12 de 548
1.

Infecciones respiratorias agudas bajas: descripción de los egresos del hospital Roberto del Río en el año 2016/ Acute respiratory infections low: description of the discharges of the hospital Roberto del Río in 2016

Autor(es): H. Yañez, Alexis; I. Tapia, Lorena; Benadof, Dona; Palomino, María Angélica
Fonte: Rev. pediatr. electrón;14(1): 50-54, 2017. tab, graf
LILACS - Literatura Latino-Americana e do Caribe em Ciências da Saúde ID: 968203
Resumo: Las infecciones respiratorias agudas bajas (IRAB) son la principal causa de hospitalización en lactantes, y una de las principales causas de muerte de niños entre un mes y 4 años. Constituyen un problema de salud pública durante los meses de otoño e invierno, con una sobredemanda de atención en los servicios de urgencia y requerimiento de camas en los distintos centros hospitalarios, guardando este fenómeno directa relación con la epidemia del virus respiratorio sincicial (VRS), el (mais)
2.

Whole-Genome Sequencing: Manual Library Preparation.

Autor(es): Mardis, Elaine; McCombie, W Richard
Fonte: Cold Spring Harb Protoc;2017(1)2017 01 03.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27803280
Resumo: This protocol describes a manual approach for the preparation of genomic DNA libraries suitable for Illumina sequencing. Genomic DNA fragments produced by shearing by sonication are ligated to adaptors and amplified by polymerase chain reaction (PCR). The amplified DNA, separated by size and gel-purified, is suitable for use as template in whole-genome sequencing.
3.

Monitoramento de vírus respiratórios na região metropolitana de Belo Horizonte, 2011 a 2013/ Monitoreo de vírus respiratórios en la región metropolitana de Belo Horizonte, 2011-2013/ Monitoring respiratory virus infection in the metropolitan area of Belo Horizonte, Brazil, 2011-2013

Autor(es): Monteiro, Cristiane Campos; Dezanet, Lorenza Nogueira Campos; França, Elisabeth Barboza
Fonte: Epidemiol. serv. saúde;25(2): 233-242, abr.-jun. 2016. tab, graf
LILACS - Literatura Latino-Americana e do Caribe em Ciências da Saúde ID: 785217
Resumo: OBJETIVO: analisar a circulação dos vírus respiratórios em residentes na região metropolitana de Belo Horizonte, Brasil, hospitalizados em Belo Horizonte, de 2011 a 2013. MÉTODOS: estudo descritivo de 5.158 indivíduos com síndrome respiratória aguda grave; foram comparadas as características dos casos confirmados com casos descartados ou sem coleta de swab. RESULTADOS: metade dos vírus isolados foi da influenza A, especialmente os subtipos A(H1N1)pdm09 em pessoas de 20-59 anos e (mais)
4.

Ring test evaluation of the detection of influenza A virus in swine oral fluids by real-time reverse-transcription polymerase chain reaction and virus isolation.

Autor(es): Goodell, Christa K; Zhang, Jianqiang; Strait, Erin; Harmon, Karen; Patnayak, Devi; Otterson, Tracy; Culhane, Marie; Christopher-Hennings, Jane; Clement, Travis; Leslie-Steen, Pamela; Hesse, Richard; Anderson, Joe; Skarbek, Kevin; Vincent, Amy; Kitikoon, Pravina; Swenson, Sabrina; Jenkins-Moore, Melinda; McGill, Jodi; Rauh, Rolf; Nelson, William; O'Connell, Catherine; Shah, Rohan; Wang, Chong; Main, Rodger; Zimmerman, Jeffrey J
Fonte: Can J Vet Res;80(1): 12-20, 2016 Jan.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26733728
Resumo: The probability of detecting influenza A virus (IAV) in oral fluid (OF) specimens was calculated for each of 13 assays based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) and 7 assays based on virus isolation (VI). The OF specimens were inoculated with H1N1 or H3N2 IAV and serially diluted 10-fold (10(-1) to 10(-8)). Eight participating laboratories received 180 randomized OF samples (10 replicates × 8 dilutions × 2 IAV subtypes plus 20 IAV-negative samples) and p (mais)
5.

Multiplex RT-PCR detection of H3N2 influenza A virus in dogs.

Autor(es): Lee, EunJung; Kim, Eun-Ju; Kim, Bo-Hye; Song, Jae-Young; Cho, In-Soo; Shin, Yeun-Kyung
Fonte: Mol Cell Probes;30(1): 56-60, 2016 Feb.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26738688
Resumo: A multiplex RT-PCR (mRT-PCR) assay to detect H3N2 CIV genomic segments was developed as a rapid and cost-effective method. Its performance was evaluated with forty-six influenza A viruses from different hosts using three primer sets which amplify four segments of H3N2 CIV simultaneously. The mRT-PCR has been successful in detecting the viral segments, indicating that it can improve the speed of diagnosis for H3N2 CIV and its reassortants.
6.

Estudo da viabilidade para introduzir na rotina testes de diagnóstico para infecção respiratória aguda/ Feasibility study to introduce the routine diagnostic tests for acute respiratory infection

Autor(es): Furlan, Teresa Maria
Fonte: São Paulo; s.n; 2016. [79] p. ilus, tab, graf.
LILACS - Literatura Latino-Americana e do Caribe em Ciências da Saúde ID: 870879
Resumo: Para avaliar os benefícios da comunicação rápida ao clínico do diagnóstico de vírus respiratórios, foi analisado a viabilidade econômica de 2 testes, com o tempo de entrega de resultado em 2 horas para teste rápido e 48 horas para Biologia Molecular. As amostras coletadas foram processadas utilizando técnicas convencionais e os testes disponíveis no mercado local. Foram escolhidos dois testes rápidos pelo método de imunocromatografia para quatro parâmetros analíticos: Influe (mais)
7.

Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus.

Autor(es): Lung, O; Pasick, J; Fisher, M; Buchanan, C; Erickson, A; Ambagala, A
Fonte: Transbound Emerg Dis;63(5): e395-402, 2016 Oct.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 25644051
Resumo: Classical swine fever (CSF) is an OIE-listed disease that can have a severe impact on the swine industry. User-friendly, sensitive, rapid diagnostic tests that utilize low-cost field-deployable instruments for CSF diagnosis can be useful for disease surveillance and outbreak monitoring. In this study, we describe validation of a new probe-based insulated isothermal reverse transcriptase PCR (iiRT-PCR) assay for rapid detection of classical swine fever virus (CSFV) on a compact, user-friendl (mais)
8.

Correlation Between the Interval of Influenza Virus Infectivity and Results of Diagnostic Assays in a Ferret Model.

Autor(es): Inagaki, Kengo; Song, Min-Suk; Crumpton, Jeri-Carol; DeBeauchamp, Jennifer; Jeevan, Trushar; Tuomanen, Elaine I; Webby, Richard J; Hakim, Hana
Fonte: J Infect Dis;213(3): 407-10, 2016 Feb 01.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26068783
Resumo: BACKGROUND: The relationship between influenza virus infectivity and virus shedding, based on different diagnostic methods, has not been defined. METHODS: Three donor ferrets infected with 2009 pandemic influenza A(H1N1) underwent daily quantitative culture, antigen-detection testing, and real-time reverse transcription-polymerase chain reaction (RT-PCR). Eight contacts were sequentially cohoused with each of the donors for 24 hours during days 3-10 after inoculation. RESULTS: Transmission (mais)
9.

Evaluation of saliva as diagnostic materials for influenza virus infection by PCR-based assays.

Autor(es): Sueki, Akane; Matsuda, Kazuyuki; Yamaguchi, Akemi; Uehara, Masayuki; Sugano, Mitsutoshi; Uehara, Takeshi; Honda, Takayuki
Fonte: Clin Chim Acta;453: 71-4, 2016 Jan 30.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26656311
Resumo: BACKGROUND: Immunochromatographic antigen tests have been widely used for detection of influenza virus; however its low sensitivity restricts the use of clinical materials other than nasopharyngeal swabs. Saliva is obtained non-invasively and has utility for diagnosis of influenza. Polymerase chain reaction (PCR) is not typically used for rapid testing because it is time consuming. We evaluated the utility of saliva as diagnostic materials for influenza virus infection by PCR-based assays. (mais)
10.

Detection of Rare Drug Resistance Mutations by Digital PCR in a Human Influenza A Virus Model System and Clinical Samples.

Autor(es): Whale, Alexandra S; Bushell, Claire A; Grant, Paul R; Cowen, Simon; Gutierrez-Aguirre, Ion; O'Sullivan, Denise M; Zel, Jana; Milavec, Mojca; Foy, Carole A; Nastouli, Eleni; Garson, Jeremy A; Huggett, Jim F
Fonte: J Clin Microbiol;54(2): 392-400, 2016 Feb.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26659206
Resumo: Digital PCR (dPCR) is being increasingly used for the quantification of sequence variations, including single nucleotide polymorphisms (SNPs), due to its high accuracy and precision in comparison with techniques such as quantitative PCR (qPCR) and melt curve analysis. To develop and evaluate dPCR for SNP detection using DNA, RNA, and clinical samples, an influenza virus model of resistance to oseltamivir (Tamiflu) was used. First, this study was able to recognize and reduce off-target ampli (mais)
11.

Enfermedad invasora por Streptococcus pyogenes como coinfección en un paciente con influenza A H1N1/ Invasive disease due to Streptococcus pyogenes in a patient with A H1N1 influenza infection. Report of one case

Autor(es): Guerrero S, Gonzalo; Marín S, Felipe
Fonte: Rev. méd. Chile;143(8): 1070-1075, ago. 2015. ilus, tab
LILACS - Literatura Latino-Americana e do Caribe em Ciências da Saúde ID: 762673
Resumo: Bacterial superinfection is a known complication among patients affected by viral respiratory tract infections. Streptococcus pyogenes, a major bacterial agent involved in acute tonsillopharyngitis, skin and soft tissue infections, was reported as a co-infecting microorganism during the 2009 A H1N1 influenza pandemic. We report a 65-year-old male patient who evolved with multifocal pneumonia and multiple organ failure with a fatal outcome. Influenza A H1N1 was detected by a polymerase chain (mais)
12.

Aspectos patológicos e microbiológicos das doenças respiratórias em suínos de terminação no Brasil/ Pathological and microbiological aspects of respiratory disease in fattening pigs in Brazil

Autor(es): Morés, Marcos A Z; Oliveira Filho, João X; Rebelatto, Raquel; Klein, Cátia S; Barcellos, David E N; Coldebella, Arlei; Morés, Nelson
Fonte: Pesqui. vet. bras;35(8): 725-733, Aug. 2015. tab, graf
LILACS - Literatura Latino-Americana e do Caribe em Ciências da Saúde ID: 767730
Resumo: Para avaliação dos aspectos patológicos e microbiológicos de casos clínicos de doenças respiratórias em suínos de terminação foram analisados 75 suínos doentes oriundos de 36 lotes. Suínos que apresentavam sinais clínicos respiratórios evidentes foram necropsiados para avaliação macroscópica e colheita de amostras para análise histopatológica e microbiológica. Foram realizados testes de isolamento bacteriano para as principais bactérias do sistema respiratório dos suí (mais)
Resultados  1-12 de 548