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Results  1-12 de 585

A field-deployable insulated isothermal RT-PCR assay for identification of influenza A (H7N9) shows good performance in the laboratory.

Author(s): Inui, Ken; Nguyen, Tung; Tseng, Hsin-Jou; Tsai, ChuanFu Mark; Tsai, Yun-Long; Chung, Simon; Padungtod, Pawin; Zhu, Huachen; Guan, Yi; Kalpravidh, Wantanee; Claes, Filip
Source: Influenza Other Respir Viruses;13(6): 610-617, 2019 11.
MEDLINE - International Database for Medical Literature PMID: 31487118
Abstract: BACKGROUND: Avian influenza A (H7N9) remains circulating in China. For countries at risk of introduction of H7N9, such as Vietnam, early detection of H7N9 virus is essential for the early containment of the virus. Insulated isothermal reverse transcriptase PCR (iiRT-PCR) is a portable PCR system that can be deployed under field conditions to identify pathogens at the sampling site. Applying PCR at the sampling site will greatly reduce the time to obtain a diagnostic result which allows the (more)

Missed detections of influenza A(H1)pdm09 by real-time RT-PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam.

Author(s): Hoang Vu Mai, Phuong; Ung Thi Hong, Trang; Nguyen Le Khanh, Hang; Nguyen Thanh, Thuy; Le Thi, Thanh; Nguyen Vu, Son; Nguyen Phuong, Anh; Tran Thi Thu, Huong; Vuong Duc, Cuong; Le Quynh, Mai
Source: Western Pac Surveill Response J;10(1): 32-38, 2019.
MEDLINE - International Database for Medical Literature PMID: 31110840
Abstract: Introduction: There are two methods of reverse transcription polymerase chain reaction (RT-PCR) that have been the common methods to detect influenza infections: conventional and real-time RT-PCR. From December 2017 to March 2018, several missed diagnoses of influenza A(H1)pdm09 using real-time RT-PCR were reported in northern Viet Nam. This study investigated how these missed detections occurred to determine their effect on the surveillance of influenza. Methods: The haemagglutinin (HA) se (more)

Development of a multiplex real-time RT-PCR assay for simultaneous detection and differentiation of influenza A, B, C, and D viruses.

Author(s): Zhang, Hewei; Wang, Yin; Porter, Elizabeth; Lu, Nanyan; Li, Yanhua; Yuan, Fangfeng; Lohman, Molly; Noll, Lance; Zheng, Wanglong; Stoy, Colin; Lang, Yuekun; Huber, Victor C; Ma, Wenjun; Peddireddi, Lalitha; Fang, Ying; Shi, Jishu; Anderson, Gary; Liu, Xuming; Bai, Jianfa
Source: Diagn Microbiol Infect Dis;95(1): 59-66, 2019 Sep.
MEDLINE - International Database for Medical Literature PMID: 31130238
Abstract: Influenza is a common and contagious respiratory disease caused by influenza A, B, C, and D viruses (IAV, IBV, ICV, and IDV). A multiplex real-time RT-PCR assay was developed for simultaneous detection of IAV, IBV, ICV, and IDV. The assay was designed to target unique sequences in the matrix gene of IBV and ICV, the RNA polymerase subunit PB1 of IDV, and combined with USDA and CDC IAV assays, both target the matrix gene. The host 18S rRNA gene was included as an internal control. In silico (more)

Outcomes of a Negative Rapid Influenza Diagnostic Test in Pregnant Women.

Author(s): Datkhaeva, Ilina; Has, Phinnara; Fitzgerald, Kelly; Hughes, Brenna L
Source: Am J Perinatol;36(1): 90-96, 2019 01.
MEDLINE - International Database for Medical Literature PMID: 29986345
Abstract: OBJECTIVE: The objective of this study was to evaluate the negative predictive value (NPV) of a rapid influenza diagnostic test (RIDT) compared with polymerase chain reaction (PCR) in pregnant women. STUDY DESIGN: Retrospective cohort study of pregnant women with a negative RIDT followed by confirmatory PCR for influenza A, H1N1, and B during the influenza seasons from 2012 to 2015. RESULTS: The NPV of the RIDT was 85.4% (211 of 247), 93.5% (231 of 247), and 97.9% (242 of 247) for influenza (more)

Evaluation of a Field-Deployable Insulated Isothermal Polymerase Chain Reaction Nucleic Acid Analyzer for Influenza A Virus Detection at Swine Exhibitions.

Author(s): Lauterbach, Sarah E; Nelson, Sarah N; Nolting, Jacqueline M; Trujillo, Jessie D; Richt, Jürgen A; Bowman, Andrew S
Source: Vector Borne Zoonotic Dis;19(3): 212-216, 2019 03.
MEDLINE - International Database for Medical Literature PMID: 30183529
Abstract: Rapid detection of influenza A virus (IAV) at swine exhibitions, where zoonotic transmission has occurred, can allow exhibition officials to quickly implement mitigation strategies and reduce public health risk. While laboratory diagnostic methods using PCR exist, pen-side detection of IAV can reduce lag time between sample collection and results. Portable insulated isothermal PCR (RT-iiPCR) has been used for point-of-care pathogen detection in veterinary medicine. This study compared labor (more)

Development and Evaluation of New Real-Time RT-PCR Assays for Identifying the Influenza A Virus Cluster IV H3N2 Variant.

Author(s): Saito, Shinji; Nakauchi, Mina; Takayama, Ikuyo; Nagata, Shiho; Odagiri, Takato; Kageyama, Tsutomu
Source: Jpn J Infect Dis;72(2): 127-129, 2019 Mar 25.
MEDLINE - International Database for Medical Literature PMID: 30381693
Abstract: From 2005 to July 6, 2018, a total of 435 swine-origin influenza A H3N2 variant virus (H3N2v) infections in humans were reported in the USA. The largest H3N2v outbreak in the USA occurred in 2011-2012. This virus obtained the HA gene from the human seasonal H3N2 influenza A viruses (seasonal H3N2) via human-to-swine transmission in the mid-1990s and was classified as Cluster IV H3N2v. For early detection of public health threats associated with Cluster IV H3N2v distinct from seasonal H3N2, (more)

Accelerated ISAV replication detection by cell culture methods combined with time-monitoring RT-qPCR.

Author(s): Arseneau, Jean-René; Gautreau, Chantal; Boston, Linda; Goguen, Michel L; Laflamme, Mark
Source: J Fish Dis;42(2): 257-267, 2019 Feb.
MEDLINE - International Database for Medical Literature PMID: 30488967
Abstract: Infectious salmon anaemia (ISA) is a viral disease that affects farmed Atlantic salmon (Salmo salar L.), often leading to mass mortalities. A quick detection of the ISA virus (ISAV) is crucial for decision-making and can prevent the occurrence of future outbreaks. Screening done by Canada's National Aquatic Animal Health Laboratory System (NAAHLS) uses quantitative reverse transcription PCR (RT-qPCR) followed by sequencing of PCR amplicons. As neither technique provides information regardin (more)

Influenza A/H1N1 pdm 09 in children's Hospital Bechir Hamza of Tunis.

Author(s): Tinsa, Faten; Barakizou, Hajer; Belhadj, Imen; Fitouri, Zohra; Borgi, Aida; Samoud, Azza; Ben Becher, Saida; Ben Jabbalah, Najla; Bouyahia, Olfa; Boussetta, Khadija
Source: Tunis Med;96(6): 348-352, 2018 Jun.
MEDLINE - International Database for Medical Literature PMID: 30430472
Abstract: BACKGROUND: Influenza A (H1N1) is a contagious acute respiratory infection caused a pandemic in 2009. The outcome was variable among populations. AIM: To describe a clinical spectrum and the outcome of Tunisian children with pandemic H1N1/09 influenza virus. METHODS: This is a retrospective, descriptive study of children with pandemic H1N1/09 influenza virus hospitalized in the children's hospital of tunis, between November 2009 and February 2010. The diagnosis was made on positive rapid te (more)

Quantitative RT-PCR Analysis of Influenza Virus Endocytic Escape.

Author(s): Su, Wen-Chi; Lai, Michael M C
Source: Methods Mol Biol;1836: 185-194, 2018.
MEDLINE - International Database for Medical Literature PMID: 30151574
Abstract: Although several virus families are internalized into their host cells by direct fusion of the viral envelope with the plasma membrane, most viruses, for example, influenza virus, make use of endocytic pathways for productive entry and infection. After endocytosis, the influenza virus escapes from the endocytic compartment to the cytosol. The distribution of the incoming influenza virus could be traced by detection of the viral RNA in the distinct cellular compartments, including endosome, (more)

Initial performance evaluation of a spotted array Mobile Analysis Platform (MAP) for the detection of influenza A/B, RSV, and MERS coronavirus.

Author(s): Hardick, Justin; Metzgar, David; Risen, Lisa; Myers, Christopher; Balansay, Melinda; Malcom, Trent; Rothman, Richard; Gaydos, Charlotte
Source: Diagn Microbiol Infect Dis;91(3): 245-247, 2018 Jul.
MEDLINE - International Database for Medical Literature PMID: 29550057
Abstract: Clinical samples were evaluated with the Mobile Analysis Platform (MAP) to determine platform performance for detecting respiratory viruses in samples previously characterized using clinical reverse transcriptase polymerase chain reaction assays. The percent agreement between MAP and clinical results was 97% for influenza A (73/75), 100% (21/21) for influenza B, 100% (6/6) for respiratory syncytial virus (RSV), and 80% (4/5) for negative specimens. The approximate limit of detection of the (more)

The Drift in Molecular Testing for Influenza: Mutations Affecting Assay Performance.

Author(s): Stellrecht, Kathleen A
Source: J Clin Microbiol;56(3)2018 03.
MEDLINE - International Database for Medical Literature PMID: 29305549
Abstract: Influenza is associated with rapid evolution due to lack of RNA polymerase proofreading, immunogenic selection, and frequent rearrangement of gene segments. Evolutionary changes affecting the performance of diagnostic testing have long been recognized. Hence, it is not surprising that such challenges apply to nucleic acid amplification tests, even though they are designed to target highly conserved regions. Initially, case reports involved single isolates of A(H1N1)pdm09. Over the past 4 ye (more)

Comparison of Cepheid® Xpert Flu and Roche RealTime Ready Influenza A/H1N1 Detection Set for detection of influenza A/H1N1.

Author(s): Rabaan, Ali A; Bazzi, Ali M; Alshaikh, Sana A
Source: Diagn Microbiol Infect Dis;90(4): 280-285, 2018 Apr.
MEDLINE - International Database for Medical Literature PMID: 29329754
Abstract: OBJECTIVE: To compare two influenza polymerase chain reaction (PCR) methods. METHODS: A total of 749 suspected MERS-CoV patients presenting at Johns Hopkins Aramco Healthcare, Saudi Arabia, each submitted a clinical sample for influenza A reflex testing using the on-site Cepheid® Xpert Flu assay and at the Ministry of Health laboratory by the Roche PCR assay. RESULTS: There was 92.12% overall agreement between the two methods. Specificity of the Cepheid® Xpert Flu was 95.8% for H1N1 and 9 (more)
Results  1-12 de 585