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Pesquisa | Influenza A (H1N1)

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Resultados  1-12 de 601

Reliability of pulse palpation in the detection of atrial fibrillation in an elderly population.

Autor(es): Jaakkola, Jussi; Vasankari, Tuija; Virtanen, Raine; Juhani Airaksinen, K E
Fonte: Scand J Prim Health Care;35(3): 293-298, 2017 Sep.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 28784027
Resumo: PURPOSE: Atrial fibrillation (AF) may first present as an ischemic stroke. Pulse palpation is a potential screening method for asymptomatic AF. We aimed to assess the reliability of pulse palpation by the elderly in detecting AF. MATERIALS AND METHODS: After brief information and training session conducted by a nurse, 173 subjects aged ≥75 years were instructed to palpate their pulse regularly for a month. After this, their ability to distinguish sinus rhythm (SR), SR with premature v (mais)

C-reactive protein and interleukin 6 microfluidic immunoassays with on-chip pre-stored reagents and centrifugo-pneumatic liquid control.

Autor(es): Zhao, Y; Czilwik, G; Klein, V; Mitsakakis, K; Zengerle, R; Paust, N
Fonte: Lab Chip;17(9): 1666-1677, 2017 05 02.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 28426080
Resumo: We present a fully automated centrifugal microfluidic method for particle based protein immunoassays. Stick-pack technology is employed for pre-storage and release of liquid reagents. Quantitative layout of centrifugo-pneumatic particle handling, including timed valving, switching and pumping is assisted by network simulations. The automation is exclusively controlled by the spinning frequency and does not require any additional means. New centrifugal microfluidic process chains are develop (mais)

What is the clinical significance of anti-Sm antibodies in systemic lupus erythematosus? A comparison with anti-dsDNA antibodies and C3.

Autor(es): Flechsig, Alexandra; Rose, Thomas; Barkhudarova, Fidan; Strauss, Romy; Klotsche, Jens; Dähnrich, Cornelia; Schlumberger, Wolfgang; Enghard, Philipp; Burmester, Gerd-Rüdiger; Hiepe, Falk; Biesen, Robert
Fonte: Clin Exp Rheumatol;35(4): 598-606, 2017 Jul-Aug.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 28281463
Resumo: OBJECTIVES: To investigate the clinical value of anti-Sm antibodies in diagnosis and monitoring of systemic lupus erythematosus (SLE) and their ability to predict lupus flares compared with that of anti-dsDNA antibody and complement (C3) assays. METHODS: Autoantibodies against Smith antigen (Sm) and double-stranded DNA (dsDNA) in sera from SLE (n=232), myositis (n=26), systemic sclerosis (n=81), Sjögren's syndrome (n=88), and rheumatoid arthritis patients (n=165) and healthy donors (n=400) (mais)

Versatile, sensitive liquid chromatography mass spectrometry - Implementation of 10 µm OT columns suitable for small molecules, peptides and proteins.

Autor(es): Vehus, T; Roberg-Larsen, H; Waaler, J; Aslaksen, S; Krauss, S; Wilson, S R; Lundanes, E
Fonte: Sci Rep;6: 37507, 2016 11 29.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27897190
Resumo: We have designed a versatile and sensitive liquid chromatographic (LC) system, featuring a monolithic trap column and a very narrow (10 µm ID) fused silica open tubular liquid chromatography (OTLC) separation column functionalized with C -groups, for separating a wide range of molecules (from small metabolites to intact proteins). Compared to today's capillary/nanoLC approaches, our system provides significantly enhanced sensitivity (up to several orders) with matching or improved separati (mais)

Multiplex RT-PCR detection of H3N2 influenza A virus in dogs.

Autor(es): Lee, EunJung; Kim, Eun-Ju; Kim, Bo-Hye; Song, Jae-Young; Cho, In-Soo; Shin, Yeun-Kyung
Fonte: Mol Cell Probes;30(1): 56-60, 2016 Feb.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26738688
Resumo: A multiplex RT-PCR (mRT-PCR) assay to detect H3N2 CIV genomic segments was developed as a rapid and cost-effective method. Its performance was evaluated with forty-six influenza A viruses from different hosts using three primer sets which amplify four segments of H3N2 CIV simultaneously. The mRT-PCR has been successful in detecting the viral segments, indicating that it can improve the speed of diagnosis for H3N2 CIV and its reassortants.

Utility of dual source CT with ECG-triggered high-pitch spiral acquisition (Flash Spiral Cardio mode) to evaluate morphological features of ventricles in children with complex congenital heart defects.

Autor(es): Nakagawa, Motoo; Ozawa, Yoshiyuki; Nomura, Norikazu; Inukai, Sachiko; Tsubokura, Satoshi; Sakurai, Keita; Shimohira, Masashi; Ogawa, Masaki; Shibamoto, Yuta
Fonte: Jpn J Radiol;34(4): 284-91, 2016 Apr.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26820980
Resumo: PURPOSE: We evaluated the ability of dual source CT (DSCT) with ECG-triggered high-pitch spiral acquisition (Flash Spiral Cardio mode) to depict the morphological features of ventricles in pediatric patients with congenital heart defects (CHD). MATERIALS AND METHODS: Between July 2013 and April 2015, 78 pediatric patients with CHD (median age 4 months) were examined using DSCT with the Flash Spiral Cardio mode. The types of ventricular abnormalities were ventricular septal defect (VSD) in (mais)

Estudo da viabilidade para introduzir na rotina testes de diagnóstico para infecção respiratória aguda/ Feasibility study to introduce the routine diagnostic tests for acute respiratory infection

Autor(es): Furlan, Teresa Maria
Fonte: São Paulo; s.n; 2016. [79] p. ilus, tab, graf.
LILACS - Literatura Latino-Americana e do Caribe em Ciências da Saúde ID: 870879
Resumo: Para avaliar os benefícios da comunicação rápida ao clínico do diagnóstico de vírus respiratórios, foi analisado a viabilidade econômica de 2 testes, com o tempo de entrega de resultado em 2 horas para teste rápido e 48 horas para Biologia Molecular. As amostras coletadas foram processadas utilizando técnicas convencionais e os testes disponíveis no mercado local. Foram escolhidos dois testes rápidos pelo método de imunocromatografia para quatro parâmetros analíticos: Influe (mais)

Evaluation of Screening Assays for the Detection of Influenza A Virus Serum Antibodies in Swine.

Autor(es): Goodell, C K; Prickett, J; Kittawornrat, A; Johnson, J; Zhang, J; Wang, C; Zimmerman, J J
Fonte: Transbound Emerg Dis;63(1): 24-35, 2016 Feb.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 24571447
Resumo: Increased surveillance of influenza A virus (IAV) infections in human and swine populations is mandated by public health and animal health concerns. Antibody assays have proven useful in previous surveillance programmes because antibodies provide a record of prior exposure and the technology is inexpensive. The objective of this research was to compare the performance of influenza serum antibody assays using samples collected from pigs (vaccinated or unvaccinated) inoculated with either A/S (mais)

Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus.

Autor(es): Lung, O; Pasick, J; Fisher, M; Buchanan, C; Erickson, A; Ambagala, A
Fonte: Transbound Emerg Dis;63(5): e395-402, 2016 Oct.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 25644051
Resumo: Classical swine fever (CSF) is an OIE-listed disease that can have a severe impact on the swine industry. User-friendly, sensitive, rapid diagnostic tests that utilize low-cost field-deployable instruments for CSF diagnosis can be useful for disease surveillance and outbreak monitoring. In this study, we describe validation of a new probe-based insulated isothermal reverse transcriptase PCR (iiRT-PCR) assay for rapid detection of classical swine fever virus (CSFV) on a compact, user-friendl (mais)

Correlation Between the Interval of Influenza Virus Infectivity and Results of Diagnostic Assays in a Ferret Model.

Autor(es): Inagaki, Kengo; Song, Min-Suk; Crumpton, Jeri-Carol; DeBeauchamp, Jennifer; Jeevan, Trushar; Tuomanen, Elaine I; Webby, Richard J; Hakim, Hana
Fonte: J Infect Dis;213(3): 407-10, 2016 Feb 01.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26068783
Resumo: BACKGROUND: The relationship between influenza virus infectivity and virus shedding, based on different diagnostic methods, has not been defined. METHODS: Three donor ferrets infected with 2009 pandemic influenza A(H1N1) underwent daily quantitative culture, antigen-detection testing, and real-time reverse transcription-polymerase chain reaction (RT-PCR). Eight contacts were sequentially cohoused with each of the donors for 24 hours during days 3-10 after inoculation. RESULTS: Transmission (mais)

Simple, rapid detection of influenza A (H1N1) viruses using a highly sensitive peptide-based molecular beacon.

Autor(es): Lim, Eun-Kyung; Guk, Kyeonghye; Kim, Hyeran; Chung, Bong-Hyun; Jung, Juyeon
Fonte: Chem Commun (Camb);52(1): 175-8, 2016 Jan 04.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26509476
Resumo: A peptide-based molecular beacon (PEP-MB) was prepared for the simple, rapid, and specific detection of H1N1 viruses using a fluorescence resonance energy transfer (FRET) system. The PEP-MB exhibited minimal fluorescence in its "closed" hairpin structure. However, in the presence of H1N1 viruses, the specific recognition of the hemagglutinin (HA) protein of H1 strains by the PEP-MB causes the beacon to assume an "open" structure that emits strong fluorescence. The PEP-MB could detect H1N1 v (mais)

Evaluation of twenty-two rapid antigen detection tests in the diagnosis of Equine Influenza caused by viruses of H3N8 subtype.

Autor(es): Yamanaka, Takashi; Nemoto, Manabu; Bannai, Hiroshi; Tsujimura, Koji; Kondo, Takashi; Matsumura, Tomio; Gildea, Sarah; Cullinane, Ann
Fonte: Influenza Other Respir Viruses;10(2): 127-33, 2016 Mar.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26568369
Resumo: BACKGROUND: Equine influenza (EI) is a highly contagious disease caused by viruses of the H3N8 subtype. The rapid diagnosis of EI is essential to reduce the disease spread. Many rapid antigen detection (RAD) tests for diagnosing human influenza are available, but their ability to diagnose EI has not been systematically evaluated. OBJECTIVES: The aim of this study was to compare the performance of 22 RAD tests in the diagnosis of EI. METHODS: The 22 RAD tests were performed on fivefold seria (mais)
Resultados  1-12 de 601