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Resultados  1-12 de 697

Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.

Autor(es): LeBlanc, J J; ElSherif, M; Mulpuru, S; Warhuus, M; Ambrose, A; Andrew, M; Boivin, G; Bowie, W; Chit, A; Dos Santos, G; Green, K; Halperin, S A; Hatchette, T F; Ibarguchi, B; Johnstone, J; Katz, K; Langley, J M; Lagacé-Wiens, P; Loeb, M; Lund, A; MacKinnon-Cameron, D; McCarthy, A; McElhaney, J E; McGeer, A; Poirier, A; Powis, J; Richardson, D; Semret, M; Shinde, V; Smyth, D; Trottier, S; Valiquette, L; Webster, D; Ye, L; McNeil, S A
Fonte: J Med Microbiol;69(2): 256-264, 2020 Feb.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31264957
Resumo: Background. The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 ( identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identif (mais)

A field-deployable insulated isothermal RT-PCR assay for identification of influenza A (H7N9) shows good performance in the laboratory.

Autor(es): Inui, Ken; Nguyen, Tung; Tseng, Hsin-Jou; Tsai, ChuanFu Mark; Tsai, Yun-Long; Chung, Simon; Padungtod, Pawin; Zhu, Huachen; Guan, Yi; Kalpravidh, Wantanee; Claes, Filip
Fonte: Influenza Other Respir Viruses;13(6): 610-617, 2019 11.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31487118
Resumo: BACKGROUND: Avian influenza A (H7N9) remains circulating in China. For countries at risk of introduction of H7N9, such as Vietnam, early detection of H7N9 virus is essential for the early containment of the virus. Insulated isothermal reverse transcriptase PCR (iiRT-PCR) is a portable PCR system that can be deployed under field conditions to identify pathogens at the sampling site. Applying PCR at the sampling site will greatly reduce the time to obtain a diagnostic result which allows the (mais)

High performance of rapid influenza diagnostic test and variable effectiveness of influenza vaccines in Mexico.

Autor(es): Castillejos, Manuel; Cabello-Gutiérrez, Carlos; Alberto Choreño-Parra, José; Hernández, Víctor; Romo, Javier; Hernández-Sánchez, Fernando; Martínez, Dina; Hernández, Andrés; Jiménez-Álvarez, Luis; Hernández-Cardenas, Carmen M; Becerril-Vargas, Eduardo; Martínez-Orozco, José A; Luis Sandoval-Gutiérrez, José; Guadarrama, Cristóbal; Olvera-Masetto, Enrique; Alfaro-Ramos, Leticia; Cruz-Lagunas, Alfredo; Ramírez, Gustavo; Márquez, Eduardo; Pimentel, Lisa; Regino-Zamarripa, Nora E; Mendoza-Milla, Criselda; Goodina, Aminadab; Hernández-Montiel, Erika; Barquera, Rodrigo; Santibañez, Alfredo; Domínguez-Cherit, Guillermo; Pérez-Padilla, Rogelio; Regalado, Justino; Santillán-Doherty, Patricio; Salas-Hernández, Jorge; Zúñiga, Joaquín
Fonte: Int J Infect Dis;89: 87-95, 2019 Dec.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31493523
Resumo: OBJECTIVES: To evaluate the performance of rapid influenza diagnostic tests (RIDT) and influenza vaccines' effectiveness (VE) during an outbreak setting. METHODS: We compared the performance of a RIDT with RT-PCR for influenza virus detection in influenza-like illness (ILI) patients enrolled during the 2016/17 season in Mexico City. Using the test-negative design, we estimated influenza VE in all participants and stratified by age, virus subtype, and vaccine type (trivalent vs quadrivalent (mais)

The effect of H1N1 vaccination on serum miRNA expression in children: A tale of caution for microRNA microarray studies.

Autor(es): Drury, Ruth Elizabeth; Pollard, Andrew John; O'Connor, Daniel
Fonte: PLoS One;14(8): e0221143, 2019.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31430297
Resumo: BACKGROUND: MicroRNAs (miRNAs) are a class of small regulatory RNAs around 21-25 nucleotides in length which govern many aspects of immunity including the host innate and adaptive responses to infection. RT-qPCR studies of select microRNAs show that vaccination alters the expression circulating microRNAs but the effect of vaccination on the global microRNA population (i.e. micronome) has never been studied. AIM: To describe vaccine associated changes in the expression of microRNAs 21 days a (mais)

Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases.

Autor(es): Xiao, Qian; Yan, Liping; Yao, Lu; Lei, Jing; Bi, Zhenwei; Hu, Jianhua; Chen, Yuqing; Fang, An; Li, Hui; Li, Yuan; Yan, Yan; Zhou, Jiyong
Fonte: BMC Vet Res;15(1): 253, 2019 Jul 19.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31324180
Resumo: BACKGROUND: Avian influenza virus (AIV), infectious bronchitis virus (IBV), and Newcastle disease virus (NDV) are important avian pathogens that can cause enormous economic loss on the poultry industry. Different respiratory etiological agents may induce similar clinical signs that make differential diagnosis difficult. Importantly, AIV brings about severe threat to human public health. Therefore, a novel method that can distinguish these viruses quickly and simultaneously is urgently neede (mais)

Magnetic SERS Strip for Sensitive and Simultaneous Detection of Respiratory Viruses.

Autor(es): Wang, Chongwen; Wang, Chaoguang; Wang, Xiaolong; Wang, Keli; Zhu, Yanhui; Rong, Zhen; Wang, Weiyun; Xiao, Rui; Wang, Shengqi
Fonte: ACS Appl Mater Interfaces;11(21): 19495-19505, 2019 May 29.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31058488
Resumo: Rapid and early diagnosis of respiratory viruses is key to preventing infections from spreading and guiding treatments. Here, we developed a sensitive and quantitative surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-based LFIA) strip for simultaneous detection of influenza A H1N1 virus and human adenovirus (HAdV) by using Fe3O4@Ag nanoparticles as magnetic SERS nanotags. The new type of Fe3O4@Ag magnetic tags, which were conjugated with dual-layer Raman dye molecules (mais)

Development of a multiplex real-time RT-PCR assay for simultaneous detection and differentiation of influenza A, B, C, and D viruses.

Autor(es): Zhang, Hewei; Wang, Yin; Porter, Elizabeth; Lu, Nanyan; Li, Yanhua; Yuan, Fangfeng; Lohman, Molly; Noll, Lance; Zheng, Wanglong; Stoy, Colin; Lang, Yuekun; Huber, Victor C; Ma, Wenjun; Peddireddi, Lalitha; Fang, Ying; Shi, Jishu; Anderson, Gary; Liu, Xuming; Bai, Jianfa
Fonte: Diagn Microbiol Infect Dis;95(1): 59-66, 2019 Sep.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31130238
Resumo: Influenza is a common and contagious respiratory disease caused by influenza A, B, C, and D viruses (IAV, IBV, ICV, and IDV). A multiplex real-time RT-PCR assay was developed for simultaneous detection of IAV, IBV, ICV, and IDV. The assay was designed to target unique sequences in the matrix gene of IBV and ICV, the RNA polymerase subunit PB1 of IDV, and combined with USDA and CDC IAV assays, both target the matrix gene. The host 18S rRNA gene was included as an internal control. In silico (mais)

Hemagglutinin gene based biosensor for early detection of swine flu (H1N1) infection in human.

Autor(es): Mohan, Hari; Gill, Paramjeet Singh; Kumar, Ashok
Fonte: Int J Biol Macromol;130: 720-726, 2019 Jun 01.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 30822474
Resumo: Hemagglutinin (HA) is a glycoprotein found on the surface of influenza A subtype virus H1N1 which play a major role in infection to the human by binding the virus to cells with sialic acid on the membrane of upper respiratory tract or erythrocytes. Based on sequence of HA gene an impedimetric biosensor was developed by immobilizing amino labeled single stranded DNA probe onto cysteine modified gold surface of the screen printed electrode for early and rapid detection of H1N1 (Swine flu) in (mais)

An electrochemical immunosensor for the corona virus associated with the Middle East respiratory syndrome using an array of gold nanoparticle-modified carbon electrodes.

Autor(es): Layqah, Laila Ali; Eissa, Shimaa
Fonte: Mikrochim Acta;186(4): 224, 2019 03 07.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 30847572
Resumo: The Middle East respiratory syndrome corona virus (MERS-CoV) is highly pathogenic. An immunosensor for the determination of MERS-CoV is described here. It is based on a competitive assay carried out on an array of carbon electrodes (DEP) modified with gold nanoparticles. Recombinant spike protein S1 was used as a biomarker for MERS CoV. The electrode array enables multiplexed detection of different CoVs. The biosensor is based on indirect competition between free virus in the sample and imm (mais)

Evaluation of the sentinel surveillance system for influenza-like illnesses in the Greater Accra region, Ghana, 2018.

Autor(es): Nuvey, Francis Sena; Edu-Quansah, Elijah Paa; Kuma, George Khumalo; Eleeza, John; Kenu, Ernest; Sackey, Samuel; Ameme, Donne; Abakar, Mahamat Fayiz; Kreppel, Katharina; Ngandolo, Richard Bongo; Afari, Edwin; Bonfoh, Bassirou
Fonte: PLoS One;14(3): e0213627, 2019.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 30870489
Resumo: BACKGROUND: Influenza-like Illness (ILI) is a medical diagnosis of possible influenza or another respiratory illness with a common set of symptoms. The deaths of four schoolchildren, during a pandemic influenza outbreak in December 2017 in Ghana, raised doubts about the ILI surveillance system's performance. We evaluated the ILI surveillance system in the Greater Accra region, Ghana, to assess the system's attributes and its performance on set objectives. METHODS: CDC guidelines were used t (mais)

Outcomes of a Negative Rapid Influenza Diagnostic Test in Pregnant Women.

Autor(es): Datkhaeva, Ilina; Has, Phinnara; Fitzgerald, Kelly; Hughes, Brenna L
Fonte: Am J Perinatol;36(1): 90-96, 2019 01.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 29986345
Resumo: OBJECTIVE: The objective of this study was to evaluate the negative predictive value (NPV) of a rapid influenza diagnostic test (RIDT) compared with polymerase chain reaction (PCR) in pregnant women. STUDY DESIGN: Retrospective cohort study of pregnant women with a negative RIDT followed by confirmatory PCR for influenza A, H1N1, and B during the influenza seasons from 2012 to 2015. RESULTS: The NPV of the RIDT was 85.4% (211 of 247), 93.5% (231 of 247), and 97.9% (242 of 247) for influenza (mais)

Evaluation of a Field-Deployable Insulated Isothermal Polymerase Chain Reaction Nucleic Acid Analyzer for Influenza A Virus Detection at Swine Exhibitions.

Autor(es): Lauterbach, Sarah E; Nelson, Sarah N; Nolting, Jacqueline M; Trujillo, Jessie D; Richt, Jürgen A; Bowman, Andrew S
Fonte: Vector Borne Zoonotic Dis;19(3): 212-216, 2019 03.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 30183529
Resumo: Rapid detection of influenza A virus (IAV) at swine exhibitions, where zoonotic transmission has occurred, can allow exhibition officials to quickly implement mitigation strategies and reduce public health risk. While laboratory diagnostic methods using PCR exist, pen-side detection of IAV can reduce lag time between sample collection and results. Portable insulated isothermal PCR (RT-iiPCR) has been used for point-of-care pathogen detection in veterinary medicine. This study compared labor (mais)
Resultados  1-12 de 697