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Pesquisa | Influenza A (H1N1)

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Resultados  1-12 de 1.557

Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus.

Autor(es): Du, Taofeng; Zhu, Guang; Wu, Xiaoping; Fang, Junyang; Zhou, En-Min
Fonte: Int J Nanomedicine;14: 9337-9349, 2019.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31819435
Resumo: Background: Enzyme-linked immunosorbent assay (ELISA) is a common method for diagnosing swine influenza. However, the production of classical antibodies is both costly and time-consuming. As a promising alternative diagnostic tool, single-domain antibodies (sdAbs) offer the advantages of simpler and faster generation, good stability and solubility, and high affinity and specificity. Methods: Phage display technology was used to isolate sdAbs against the SIV-NP protein from a camel VHH libra (mais)

Molecular characterization of influenza A(H1N1)pdm09 viruses circulating at various geographical locations in India, 2017.

Autor(es): Potdar, Varsha; Vijay, Neetu; Gupta, Nivedita; Arunkumar, G; Borkakoty, Bishwajyoti; Malhotra, Bharti; Rabha, Debajit; Hinge, Dilip; Kaur, Harmanmeet; Chadha, Mandeep
Fonte: Indian J Med Res;149(6): 783-789, 2019 06.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31496532
Resumo: Background & objectives: Influenza virological surveillance is an essential tool for the early detection of novel genetic variants of epidemiologic and clinical significance. This study was aimed to genetically characterize A(H1N1)pdm09 virus circulating in 2017 and to compare it with the global data. Methods: The regional/State Viral Research and Diagnostic Laboratories (VRDLs) provided influenza diagnosis for referred clinical samples and shared influenza A(H1N1)pdm09 positives with the I (mais)

Mucosal CD8+ T cell responses induced by an MCMV based vaccine vector confer protection against influenza challenge.

Autor(es): Zheng, Xiaoyan; Oduro, Jennifer D; Boehme, Julia D; Borkner, Lisa; Ebensen, Thomas; Heise, Ulrike; Gereke, Marcus; Pils, Marina C; Krmpotic, Astrid; Guzmán, Carlos A; Bruder, Dunja; Cicin-Sain, Luka
Fonte: PLoS Pathog;15(9): e1008036, 2019 09.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31525249
Resumo: Cytomegalovirus (CMV) is a ubiquitous ß-herpesvirus that establishes life-long latent infection in a high percentage of the population worldwide. CMV induces the strongest and most durable CD8+ T cell response known in human clinical medicine. Due to its unique properties, the virus represents a promising candidate vaccine vector for the induction of persistent cellular immunity. To take advantage of this, we constructed a recombinant murine CMV (MCMV) expressing an MHC-I restricted epitop (mais)

NSP Protein Encoded in Negative NS RNA Strand of Influenza A Virus Induces Cellular Immune Response in Infected Animals.

Autor(es): Zhirnov, O P; Isaeva, E I
Fonte: Dokl Biochem Biophys;486(1): 201-205, 2019 May.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31367821
Resumo: Infection of mice with influenza A viruses led to the formation of clones of lymphocytes that specifically recognizes viral domains in the central zone of the NSP protein (amino acid positions 83-119). Computer analysis of the primary structure of the NSP protein showed the presence of T-cell epitopes in the central part of the NSP molecule. The findings indicate that the viral NSP gene is expressed in the infected animals and verify the concept of the bipolar strategy (ambisense strategy) of the influenza A virus genome.

Priming and realignment by the influenza a virus RdRp is dependent on the length of the host primers and the extent of base pairing to viral RNA.

Autor(es): De Vlugt, Corey; Sikora, Dorota; Rocheleau, Lynda; Pelchat, Martin
Fonte: Virology;536: 91-100, 2019 10.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31404845
Resumo: Initiation of influenza A virus (IAV) transcription depends on RNA primers derived from host RNAs. During this process, some primers are elongated by a few nucleotides, realigned on the viral RNA templates (vRNA), and then used to initiate another round of transcription. Here, we used information on the host primers used by four IAV strains and four mini-replicons to investigate the characteristics of primer undergoing priming and realignment. We report that primers are biased towards this (mais)

Genetic characterisation of influenza A(H1N1)pdm09 viruses circulating in Assam, Northeast India during 2009-2015.

Autor(es): Biswas, Dipankar; Dutta, Mousumi; Sarmah, Kimmi; Yadav, Kaushal; Buragohain, Manika; Sarma, Kishore; Borkakoty, Biswajyoti
Fonte: Indian J Med Microbiol;37(1): 42-49, 2019.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31424009
Resumo: Introduction: Influenza A(H1N1)pdm09 virus, since its identification in April 2009, has continued to cause significant outbreaks of respiratory tract infections including pandemics in humans. In the course of its evolution, the virus has acquired many mutations with an ability to cause increased disease severity. A regular molecular surveillance of the virus is essential to mark the evolutionary changes that may cause a shift to the viral behavior. Materials and Methods: Samples of Throat/N (mais)

The effect of H1N1 vaccination on serum miRNA expression in children: A tale of caution for microRNA microarray studies.

Autor(es): Drury, Ruth Elizabeth; Pollard, Andrew John; O'Connor, Daniel
Fonte: PLoS One;14(8): e0221143, 2019.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31430297
Resumo: BACKGROUND: MicroRNAs (miRNAs) are a class of small regulatory RNAs around 21-25 nucleotides in length which govern many aspects of immunity including the host innate and adaptive responses to infection. RT-qPCR studies of select microRNAs show that vaccination alters the expression circulating microRNAs but the effect of vaccination on the global microRNA population (i.e. micronome) has never been studied. AIM: To describe vaccine associated changes in the expression of microRNAs 21 days a (mais)

Identification of key hemagglutinin residues responsible for cleavage, acid stability, and virulence of fifth-wave highly pathogenic avian influenza A(H7N9) viruses.

Autor(es): Sun, Xiangjie; Belser, Jessica A; Yang, Hua; Pulit-Penaloza, Joanna A; Pappas, Claudia; Brock, Nicole; Zeng, Hui; Creager, Hannah M; Stevens, James; Maines, Taronna R
Fonte: Virology;535: 232-240, 2019 09.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31325838
Resumo: We previously demonstrated that despite no airborne transmissibility increase compared to low pathogenic avian influenza viruses, select human isolates of highly pathogenic avian influenza A(H7N9) virus exhibit greater virulence in animal models and a lower threshold pH for fusion. In the current study, we utilized both in vitro and in vivo approaches to identify key residues responsible for hemagglutinin (HA) intracellular cleavage, acid stability, and virulence in mice. We found that the (mais)

Biological properties of influenza A virus mutants with amino acid substitutions in the HA2 glycoprotein of the HA1/HA2 interaction region.

Autor(es): Jakubcová, L; Vozárová, M; Hollý, J; Tomcíková, K; Fogelová, M; Polcicová, K; Kostolanský, F; Fodor, E; Varecková, E
Fonte: J Gen Virol;100(9): 1282-1292, 2019 09.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31329089
Resumo: Influenza A viruses (IAVs) enter into cells by receptor-dependent endocytosis. Subsequently, conformational changes of haemagglutinin are triggered by low environmental pH and the N terminus of HA2 glycoprotein (gp) is inserted into the endosomal membrane, resulting in fusion pore formation and genomic vRNA release into the cytoplasm. However, the pH optimum of membrane fusion is host- and virus-specific and can have an impact on virus pathogenicity. We prepared mutants of neurotropic IAV A (mais)

Integration analysis of a miRNA-mRNA expression in A549 cells infected with a novel H3N2 swine influenza virus and the 2009 H1N1 pandemic influenza virus.

Autor(es): Gao, Lingxi; Gao, Jie; Liang, Ying; Li, Rui; Xiao, Qing; Zhang, Zengfeng; Fan, Xiaohui
Fonte: Infect Genet Evol;74: 103922, 2019 10.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31207403
Resumo: Swine are reservoirs for anthropogenic/zoonotic influenza viruses, and the prevalence and repeated introduction of the 2009 H1N1 pandemic influenza virus (pdm/09) into pigs raises the possibility of generating novel swine influenza viruses with the potential to infect humans. However, studies aiming to identify miRNAs involved in the transfer of novel swine influenza virus infection to human cells are rare. In this investigation, from the view of small RNA, microarrays and high-throughput s (mais)

Deep sequencing of 2009 influenza A/H1N1 virus isolated from volunteer human challenge study participants and natural infections.

Autor(es): Xiao, Yongli; Park, Jae-Keun; Williams, Stephanie; Ramuta, Mitchell; Cervantes-Medina, Adriana; Bristol, Tyler; Smith, Sarah; Czajkowski, Lindsay; Han, Alison; Kash, John C; Memoli, Matthew J; Taubenberger, Jeffery K
Fonte: Virology;534: 96-107, 2019 08.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31226666
Resumo: Nasal wash samples from 15 human volunteers challenged with GMP manufactured influenza A/California/04/2009(H1N1) and from 5 naturally infected influenza patients of the 2009 pandemic were deep sequenced using viral targeted hybridization enrichment. Ten single nucleotide polymorphism (SNP) positions were found in the challenge virus. Some of the nonsynonymous changes in the inoculant virus were maintained in some challenge participants, but not in others, indicating that virus is evolving (mais)

Detection of multiple viral sequences in the respiratory tract samples of suspected Middle East respiratory syndrome coronavirus patients in Jakarta, Indonesia 2015-2016.

Autor(es): Setianingsih, Tri Yuli; Wiyatno, Ageng; Hartono, Teguh Sarry; Hindawati, Evi; Dewantari, Aghnianditya Kresno; Myint, Khin Saw; Lisdawati, Vivi; Safari, Dodi
Fonte: Int J Infect Dis;86: 102-107, 2019 Sep.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 31238156
Resumo: OBJECTIVES: The identification and analysis of viral etiological agents from suspected Middle East respiratory syndrome coronavirus (MERS-CoV) cases admitted to Prof. Dr. Sulianti Saroso Infectious Disease Hospital (IDH) using molecular assays. METHODS: Biological samples were collected from 13 hospitalized patients suspected of MERS-CoV infection in Prof. Dr. Sulianti Saroso IDH from July 2015 to December 2016. The majority of patients presented with pneumonia, with symptoms including feve (mais)
Resultados  1-12 de 1.557