RESUMEN
Previously we showed that 65-kDa Mycobacterium leprae heat shock protein (Hsp65) is a target for the development of a tuberculosis vaccine. Here we evaluated peripheral blood mononuclear cells (PBMC) from healthy individuals or tuberculosis patients stimulated with two forms of Hsp65 antigen, recombinant DNA that encodes Hsp65 (DNA-HSP65) or recombinant Hsp65 protein (rHsp65) in attempting to mimic a prophylactic or therapeutic study in vitro, respectively. Proliferation and cytokine-producing CD4+ or CD8+ cell were assessed by flow cytometry. The CD4+ cell proliferation from healthy individuals was stimulated by DNA-HSP65 and rHsp65, while CD8+ cell proliferation from healthy individuals or tuberculosis patients was stimulated by rHSP65. DNA-HSP65 did not improve the frequency of IFN-gamma+ cells from healthy individuals or tuberculosis patients. Furthermore, we found an increase in the frequency of IL-10-producing cells in both groups. These findings show that Hsp65 antigen activates human lymphocytes and plays an immune regulatory role that should be addressed as an additional antigen for the development of antigen-combined therapies.
Asunto(s)
Proteínas Bacterianas/inmunología , Chaperonina 60/inmunología , Inmunidad Celular , Activación de Linfocitos , Tuberculosis/inmunología , Adulto , Proteínas Bacterianas/genética , Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD8-positivos/inmunología , Chaperonina 60/genética , Citotoxicidad Inmunológica , Femenino , Voluntarios Sanos , Humanos , Interferón gamma/biosíntesis , Interferón gamma/inmunología , Interleucina-10/biosíntesis , Interleucina-10/inmunología , Leucocitos Mononucleares/inmunología , Macrófagos Alveolares/inmunología , Masculino , Persona de Mediana Edad , Mycobacterium tuberculosis/inmunología , Proteínas Recombinantes/inmunología , Vacunas contra la Tuberculosis/inmunología , Regulación hacia Arriba , Vacunas de ADN/farmacología , Adulto JovenRESUMEN
Neste trabalho, nos quantificamos os niveis de anticorpos anti-PGL-I no soro de pacientes com hanseniase multibacilar (HV e HDV), paucibacilar (HT e HDT) e em comunicantes intra e extra domiciliares. A finalidade destas determinacoes visavam: 1- a implantacao de um teste sorologico de rotina laboratorial para o diagnostico precoce desta enfermidade; 2- a monitorizacao da resposta terapeutica e da reacao tipo I; 3- estabelecimento de correlacoes com os niveis sericos de interleucinas, com o indice baciloscopico e atividade da doenca, com a reacao de Mitsuda e com reacoes hansenicas. A quantificacao dos anticorpos foi feita pelo metodo de ELISA e foi altamente especifica para a hanseniase. Observou-se baixos niveis de anticorpos anti-PGL-I nos pacientes HT