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1.
Int J Lepr Other Mycobact Dis ; 50(2): 200-4, 1982 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-6811449

RESUMO

An assay system has been developed based on automated radiometric quantification of 14CO2 produced through oxidation of [1-14C] fatty acids by mycobacteria. Two stains of M. tuberculosis (H37Rv and Erdman) and one of M. bovis (BCG) in 7H9 medium (ADC) with 1.0 microCi of one of the fatty acids (butyric, hexanoic, octanoic, decanoic, lauric, myristic, palmitic, stearic, oleic, linoleic and linolenic) were studied. Results previously published on M. lepraemurium (Hawaiian) were also included for comparison. Both strains of M. tuberculosis had maximum 14CO2 production from hexanoic acid. Oxidation of butyric and avid oxidation of lauric acids were also found with the H37Rv strain but not with Erdman. In contrast, 14CO2 production by M. bovis was greatest from lauric and somewhat less from decanoic acid. M. lepraemurium showed increasing oxidation rates from myristic, decanoic and lauric acids. Assimilation studies of M. tuberculosis H37Rv confirmed that most of the oxidized substrates were converted into by-products with no change in those from which no oxidation was found. These data suggest that the radiometric measurement of differential fatty acid metabolism may provide a basis of strain identification of the genus Mycobacterium.


Assuntos
Ácidos Graxos/metabolismo , Mycobacterium/metabolismo , Dióxido de Carbono/análise , Radioisótopos de Carbono , Meios de Cultura , Mycobacterium bovis/metabolismo , Mycobacterium lepraemurium/metabolismo , Mycobacterium tuberculosis/metabolismo , Oxirredução
2.
Int J Lepr Other Mycobact Dis ; 47(2): 126-32, 1979 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-378870

RESUMO

An assay system has been developed based on automated radiometric quantification of 14CO2 produced through oxidation of (1--14C) fatty acids by mycobacteria. With this system, the Hawaiian strain of M. lepraemurium was studied using the K-36 buffer as a suspending solution for the organisms along with 5.0 muCi of one of the following fatty acids: acetate, butyric, hexanoic, octanoic, decanoic, lauric, myristic, palmitic, stearic, oleic, linoleic, linolenic, and malonic. The 14CO2 production by this organism was greatest with lauric, decanoic, myristic, octanoic, stearic, oleic, linoleic, linolenic, and malonic. The 14CO2 production by this organism was greatest with lauric, decanoic, myristic, octanoic, and stearic acids, in decreasing order. Assimilation studies and radiochromatograms confirmed that most of the oxidized substrates were converted into by-products with no change in those from which no oxidation was found. These data suggest that the radiometric measurement of differential fatty acid metabolism may provide a basis of radiometric identification of M. lepraemurium and assessment of the growth requirements of this organism.


Assuntos
Ácidos Graxos/metabolismo , Mycobacterium lepraemurium/metabolismo , Radiometria/instrumentação , Dióxido de Carbono/metabolismo , Cromatografia em Papel , Cromatografia em Camada Delgada
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