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1.
Clin Exp Immunol ; 47(1): 53-8, 1982 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-6807589

RESUMO

The enzyme-linked immunosorbent assay (ELisa) was used to detect the presence of antibodies to muramyl dipeptide (MDP) in serum of patients with leprosy or tuberculosis. Using a conjugate of MDP-lysine to horse radish peroxidase, no such antibodies could be detected in sera of either patients or controls. Antibodies to a sonicate antigen of Mycobacterium tuberculosis were found in sera of all individuals tested and the binding of these antibodies to the M. tuberculosis antigen could not be inhibited by MDP. On the other hand, binding of MDP to anti-MDP antibodies, raised in rabbits, was largely inhibited by free MDP, slightly inhibited by M. tuberculosis antigen and was not inhibited by the patients' sera.


Assuntos
Acetilmuramil-Alanil-Isoglutamina/imunologia , Anticorpos/análise , Glicopeptídeos/imunologia , Hanseníase/imunologia , Tuberculose/imunologia , Adulto , Anticorpos/imunologia , Anticorpos Antibacterianos/análise , Anticorpos Antibacterianos/imunologia , Antígenos de Bactérias/imunologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Mycobacterium tuberculosis/imunologia
2.
Immunology ; 41(4): 865-73, 1980 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-6161879

RESUMO

A report is given of the use of the enzyme-linked immunosorbent assay to measure antibody to preparations of human thymocyte membranes (HTMA) and to beta 2-microglobulin. The assay described is simple and rapid, and requires only small quantities of an easily stored membrane preparation. The advantages of this technique over conventional methods involving cytotoxicity are discussed. Raised levels of IgM antibody to beta 2-microglobulin were detected in sera from SLE patients. Raised levels of IgG and IgM antibody to HTMA were found in sera from most active lepromatous cases. Two of eight sera from SLE patients showed raised IgG anti-HMTA, but not raised IgM. An attempt was made to study the subclass of the IgG antibodies found, but when checked against purified human IgG myeloma proteins, the available anti-subclass sera were found to lack the necessary degree of specificity in this assay.


Assuntos
Autoanticorpos/análise , beta-Globulinas/imunologia , Hanseníase/imunologia , Lúpus Eritematoso Sistêmico/imunologia , Linfócitos T/imunologia , Microglobulina beta-2/imunologia , Antígenos de Superfície/imunologia , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina G/análise , Imunoglobulina G/classificação , Imunoglobulina M/análise , Fator Reumatoide/análise
3.
s.l; s.n; dec. 1980. 9 p. graf.
Não convencional em Inglês | SES-SP, SES-SP, HANSEN, HANSENIASE, SESSP-ILSLACERVO, SES-SP | ID: biblio-1240475

RESUMO

A report is given of the use of the enzyme-linked immunosorbent assay to measure antibody to preparations of human thymocyte membranes (HTMA) and to beta 2-microglobulin. The assay described is simple and rapid, and requires only small quantities of an easily stored membrane preparation. The advantages of this technique over conventional methods involving cytotoxicity are discussed. Raised levels of IgM antibody to beta 2-microglobulin were detected in sera from SLE patients. Raised levels of IgG and IgM antibody to HTMA were found in sera from most active lepromatous cases. Two of eight sera from SLE patients showed raised IgG anti-HMTA, but not raised IgM. An attempt was made to study the subclass of the IgG antibodies found, but when checked against purified human IgG myeloma proteins, the available anti-subclass sera were found to lack the necessary degree of specificity in this assay.


Assuntos
Humanos , Autoanticorpos/análise , Ensaio de Imunoadsorção Enzimática , Hanseníase/imunologia , Imunoglobulina G/análise , Imunoglobulina G/classificação , Imunoglobulina M/análise , Linfócitos T/imunologia , Lúpus Eritematoso Sistêmico/imunologia , beta-Globulinas/imunologia
4.
Int J Lepr Other Mycobact Dis ; 47(4): 597-600, 1979 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-122630

RESUMO

The distribution of 24 histocompatibility antigens in 88 Azerbaijani patients with leprosy was determined and compared with those of 125 normal, ethnically matched individuals. HLA-BW35 was increased in frequency among the Kurdish patients as compared to the controls; HLA-A1, however, displayed decreased frequency in patients with the lepromatous form of the disease. Among the Turks, diminished frequency of HLA-BW15 was noted in the total patient population. None of these comparisons, however, reached statistical significance when corrected for the number of antigens tested. Across the two ethnic groups, differences in the frequencies of HLA antigens between the patients and the controls were only marginal.


Assuntos
Antígenos HLA/análise , Hanseníase/genética , Antígeno HLA-A1/análise , Antígenos HLA-B/análise , Antígeno HLA-B15 , Antígeno HLA-B35/análise , Humanos , Irã (Geográfico) , Hanseníase/imunologia , Hanseníase Virchowiana/genética , Hanseníase Virchowiana/imunologia
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