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J Immunol ; 188(10): 4782-91, 2012 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-22504648


Leprosy is not eradicable with currently available diagnostics or interventions, as evidenced by its stable incidence. Early diagnosis of Mycobacterium leprae infection should therefore be emphasized in leprosy research. It remains challenging to develop tests based on immunological biomarkers that distinguish individuals controlling bacterial replication from those developing disease. To identify biomarkers for field-applicable diagnostics, we determined cytokines/chemokines induced by M. leprae proteins in blood of leprosy patients and endemic controls (EC) from high leprosy-prevalence areas (Bangladesh, Brazil, Ethiopia) and from South Korea, where leprosy is not endemic anymore. M. leprae-sonicate-induced IFN-γ was similar for all groups, excluding M. leprae/IFN-γ as a diagnostic readout. By contrast, ML2478 and ML0840 induced high IFN-γ concentrations in Bangladeshi EC, which were completely absent for South Korean controls. Importantly, ML2478/IFN-γ could indicate distinct degrees of M. leprae exposure, and thereby the risk of infection and transmission, in different parts of Brazilian and Ethiopian cities. Notwithstanding these discriminatory responses, M. leprae proteins did not distinguish patients from EC in one leprosy-endemic area based on IFN-γ. Analyses of additional cytokines/chemokines showed that M. leprae and ML2478 induced significantly higher concentrations of MCP-1, MIP-1ß, and IL-1ß in patients compared with EC, whereas IFN-inducible protein-10, like IFN-γ, differed between EC from areas with dissimilar leprosy prevalence. This study identifies M. leprae-unique Ags, particularly ML2478, as biomarker tools to measure M. leprae exposure using IFN-γ or IFN-inducible protein-10, and also shows that MCP-1, MIP-1ß, and IL-1ß can potentially distinguish pathogenic immune responses from those induced during asymptomatic exposure to M. leprae.

Citocinas/sangue , Hanseníase/epidemiologia , Hanseníase/imunologia , Mycobacterium leprae/imunologia , Adulto , Idoso , Antígenos de Bactérias/imunologia , Bangladesh/epidemiologia , Biomarcadores/sangue , Brasil/epidemiologia , Citocinas/biossíntese , Citocinas/genética , Etiópia/epidemiologia , Feminino , Humanos , Interferon gama/biossíntese , Interferon gama/sangue , Interferon gama/genética , Hanseníase/diagnóstico , Masculino , Pessoa de Meia-Idade , República da Coreia/epidemiologia , Células Th1/imunologia , Células Th1/microbiologia , Células Th2/imunologia , Células Th2/microbiologia , Adulto Jovem
Int J Lepr Other Mycobact Dis ; 47(4): 597-600, 1979 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-122630


The distribution of 24 histocompatibility antigens in 88 Azerbaijani patients with leprosy was determined and compared with those of 125 normal, ethnically matched individuals. HLA-BW35 was increased in frequency among the Kurdish patients as compared to the controls; HLA-A1, however, displayed decreased frequency in patients with the lepromatous form of the disease. Among the Turks, diminished frequency of HLA-BW15 was noted in the total patient population. None of these comparisons, however, reached statistical significance when corrected for the number of antigens tested. Across the two ethnic groups, differences in the frequencies of HLA antigens between the patients and the controls were only marginal.

Antígenos HLA/análise , Hanseníase/genética , Antígeno HLA-A1/análise , Antígenos HLA-B/análise , Antígeno HLA-B15 , Antígeno HLA-B35/análise , Humanos , Irã (Geográfico) , Hanseníase/imunologia , Hanseníase Virchowiana/genética , Hanseníase Virchowiana/imunologia