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1.
Kitasato Arch Exp Med ; 64(4): 205-12, 1991 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-1823920

RESUMO

Mycobacterium leprae is an obligate intracellular parasite and grows within mononuclear phagocytes. When murine macrophages derived from the peritoneal cavities of CBA mice were infected in vitro with M. leprae (Thai-53 strain), intracellular multiplication was observed three weeks after infection. On the other hand, there was no increase in the number of heat-killed M. leprae at the same times after infection. Morphological studies showed that the growth rate of the bacteria increased by about 20-30% in medium supplemented with cycloheximide. With the addition of cycloheximide to the culture medium, metabolic activity of macrophages was decreased but infected cells were maintained in good condition and seldom floated off from the culture flask.


Assuntos
Técnicas Bacteriológicas , Cicloeximida/farmacologia , Macrófagos/microbiologia , Mycobacterium leprae/crescimento & desenvolvimento , Animais , Divisão Celular/efeitos dos fármacos , Feminino , Macrófagos/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos CBA/microbiologia
2.
Kitasato Arch Exp Med ; 64(4): 213-20, 1991 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-1823921

RESUMO

Mycobacterium leprae is an obligate intracellular parasite which grows within mononuclear phagocytes. In clinical cases, M. leprae reaches enormous numbers in the macrophage-rich granulomas of leprosy. Peritoneal macrophages from CBA mice were cultured in Waymouth medium containing fresh horse serum in Costar 3424 trays (24 wells, 16 mm in diameter) each containing 9 x 12 mm cover slips. This medium was supplemented with 0.5 micrograms/ml of cycloheximide. These cells were infected with M. leprae Thai-53 strain obtained by nude mice inoculation. Significant multiplication of the acid-fast bacilli in the macrophages was observed three weeks after inoculation. This experiment showed M. leprae mainly multiplied in cells and not by rephagocytization of M. leprae derived from destroyed cells.


Assuntos
Técnicas Bacteriológicas , Macrófagos/microbiologia , Mycobacterium leprae/crescimento & desenvolvimento , Animais , Técnicas Bacteriológicas/instrumentação , Divisão Celular/efeitos dos fármacos , Cicloeximida/farmacologia , Feminino , Macrófagos/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos CBA/microbiologia , Camundongos Nus/microbiologia , Fagocitose
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