Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 32
Plant Dis ; 90(5): 682, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-30781156


In Colombia, citrus is cultivated in mostly small plantings that total 55,000 ha by approximately 25,000 farmers. Production includes 1,200 tons of fresh fruits and 60 tons of juice for domestic consumption, resulting in a net worth of US$650,000 per year. Most of the production comes from areas located between the Cordillera Occidental and Cordillera Central mountain ranges (departments of Antioquia, Caldas, Quindio, and Risaralda) near coffee plantations. The departments of Meta and Casanare, located at the east plains (Llanos Orientales), include a zone parallel (4 to 5°N, 72 to 74°W) to the east mountain range and generate approximately 10% of the total Colombian citrus production. Suspected citrus leprosis symptoms on leaves and fruits of sweet oranges (Citrus sinensis (L.) Osb.) were first observed by plant pathologists for CORPOICA (Colombian National Agricultural Research Organization) in citrus orchards in Casanare in 2003, and later in 2004, in Meta. To confirm the visual identification, leaves and fruits from Valencia sweet orange exhibiting typical lesions of leprosis were collected from several locations in the departments of Casanare (Yopal, Aguazul) and Meta (Guamal, Villavicencio, and Cumaral). Samples were fixed in cacodylate-buffered paraformaldehyde/glutaraldehyde solution and subsequently processed for examination in thin sections using electron microscopy. Samples were processed and examined at the Citrus Research and Educational Center (CREC) of the University of Florida, Lake Alfred, and the Agricultural College (ESALQ) of the Universidade de São Paulo at Piracicaba, SP, Brazil. Some leaf samples collected in Meta were also dried and used for detection of Citrus leprosis virus, cytoplasmic type (CiLV-C) by reverse transcription-polymerase chain reaction (RT-PCR) at the Centro APTA Citros Sylvio Moreira at Cordeirópolis (CAPTACSM). The RT-PCR was performed with primers that specifically amplify a fragment of the viral genome that codes for the putative cell-to-cell movement protein (1). Locations at CREC and ESALQ each observed, using electron microscopy, cell changes characteristic of CiLV-C that include short bacilliform particles in the endoplasmic reticulum and dense, vacuolated, and irregularly shaped viroplasm in the cytoplasm (2) in samples from Casanare and Meta. RT-PCR amplified cDNA fragments of the expected size for samples collected in Meta and one of the amplicons was sequenced (GenBank Accession No. DQ272491). The sequence obtained was found to have 98% nucleotide sequence identity to the Brazilian CiLV-C isolate (GenBank Accession No. AY289190.1). Mites collected from affected plants from the department of Meta were identified at ESALQ as Brevipalpus phoenicis (Geijskes), a known principal vector of CiLV-C (2). These several lines of evidence confirmed that the symptoms observed in sweet oranges at Meta and Casanare are due to the infection by CiLV-C. To our knowledge, this is the first report of this virus in Colombia. References:(1) E. C. Locali et al. Plant Dis. 87:1317, 2003, (2) J. C. V. Rodrigues et al. Exp. Appl. Acarol. 30:161, 2003.

Lepr Rev ; 67(1): 4-12, 1996 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-8820514


A total of 23,863 individuals living in an area of low endemicity for leprosy were tested by enzyme-linked immunosorbent assay with a semisynthetic analogue of the phenolic glycolipid I antigen of Mycobacterium leprae. The proportion found positive was 3.86% which was significantly higher than that in a sample of a population known to be free of leprosy. Clinical examinations as well as Mitsuda and skin smear tests were organized for those defined as seropositive. The proportion of individuals with lepromin reactions of less than 3 mm increased 18.9% per serological interval as antibodies rose though it was not statistically significant. As a result of the clinical and bacteriological examinations, 2 cases with clinical signs and heavy bacillary load were found, whereas acid-fast bacilli were demonstrated in 2 other individuals without clinical manifestations of leprosy. The usefulness of the system for control purposes is discussed.

Anticorpos Antibacterianos/sangue , Antígenos de Bactérias , Glicolipídeos , Hanseníase/diagnóstico , Mycobacterium leprae/imunologia , Adolescente , Adulto , Idoso , Criança , Cuba/epidemiologia , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Hanseníase/sangue , Hanseníase/epidemiologia , Hanseníase/imunologia , Hanseníase/microbiologia , Masculino , Pessoa de Meia-Idade
J Antimicrob Chemother ; 32(6): 797-808, 1993 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-8144420


From the results of recently-published in-vitro studies, we have reviewed the activities of novel quinolones which are currently available, either commercially or for investigative purposes, against Mycobacterium tuberculosis, the atypical mycobacteria (principally the Mycobacterium avium complex, Mycobacterium chelonae, Mycobacterium xenopi, Mycobacterium marinum and Mycobacterium fortuitum) and Mycobacterium leprae. We have also evaluated the effects of the various methods for determining the susceptibilities of the mycobacteria on the in-vitro activities of these agents. Sparfloxacin, Win-57273, ciprofloxacin and ofloxacin were the most active agents overall. The in-vitro activities, efficacies in animal models, tissue and cell penetration and results of preliminary clinical investigations suggest that some of the newer quinolones might be effective alternatives to standard anti-tuberculous agents for the treatment of patients with infections caused by mycobacteria, particularly when there is resistance to the latter group of drugs.

Anti-Infecciosos/farmacologia , Mycobacterium leprae/efeitos dos fármacos , Mycobacterium tuberculosis/efeitos dos fármacos , Micobactérias não Tuberculosas/efeitos dos fármacos , Ciprofloxacina/farmacologia , Humanos , Testes de Sensibilidade Microbiana , Micobactérias não Tuberculosas/classificação , Norfloxacino/farmacologia , Ofloxacino/farmacologia
Lepr Rev ; 64(2): 128-35, 1993 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-8341115


A total of 1211 Cuban multibacillary leprosy patients treated for at least 5 years were clinically and bacteriologically examined. They were being treated according to a 2-phase monotherapy regimen with RMP first and DADDS afterwards. On skin-smear examination 50 patients were found positive, of which 9 showed a BI of 3+ or higher at any site. With regard to the clinical status the only cases found with clinical signs of relapse were 5 out of 7 long-standing patients with BI of 4+ and 5+. A 6th patient of this high BI group who showed a good clinical condition, except for a heavy infiltration of both earlobes, was receiving a second RMP course when examined and biopsied for this research. These 9 patients were biopsied and susceptibility tests to RMP and DDS performed. The results showed that in 1 case the Mycobacterium leprae were resistant to both drugs; the organisms from 2 other patients were susceptible to RMP but low-grade resistant to DDS. Those from another patient were susceptible to RMP and fully resistant to DDS. In 3 other cases the bacilli did not multiply in any of the mice but 1 of these strains was from the patient taking a second RMP course, therefore this strain might also be susceptible to RMP and resistant to DDS. In the last 2 cases multiplication was only observed in 2 of the controls and in 1 of the 0.0001% DDS treated mice; therefore, these experiments were not conclusive, and the AFB recovered were inoculated into fresh mice to repeat the tests but these failed to multiply.

Dapsona/farmacologia , Hanseníase/microbiologia , Mycobacterium leprae/efeitos dos fármacos , Rifampina/farmacologia , Animais , Resistência Microbiana a Medicamentos , Feminino , Humanos , Hanseníase/tratamento farmacológico , Masculino , Camundongos , Camundongos Endogâmicos BALB C
s.l; s.n; 1973. 8 p. ilus.
Não convencional em Espanhol | SES-SP, HANSEN, SESSP-ILSLACERVO, SES-SP | ID: biblio-1232561

Int. j. lepr ; 27(1): 74-74, Jan.-Marc. 1959.
Artigo em Inglês | SES-SP, HANSEN, SESSP-ILSLACERVO, SES-SP | ID: biblio-1227896
Int. j. lepr ; 26(4): 389-396, Oct.-Dec. 1958.
Artigo em Inglês | SES-SP, HANSEN, SESSP-ILSLACERVO, SES-SP | ID: biblio-1227881
s.l; s.n; 1950. 8 p.
Não convencional em Inglês | SES-SP, HANSEN, SESSP-ILSLACERVO, SES-SP | ID: biblio-1231935

Int. j. lepr ; 15(1): 109-114, Jan.-Mar. 1947.
Artigo em Inglês | SES-SP, HANSEN, SESSP-ILSLACERVO, SES-SP | ID: biblio-1227336
Int. j. lepr ; 8(3): 307-332, July-Sep. 1940. ilus
Artigo em Inglês | SES-SP, HANSEN, SESSP-ILSLACERVO, SES-SP | ID: biblio-1228371


Three cases which bear on the unsettled question of the transformation of tuberculoid leprosy to the lepromatous type are presented. Two of them have been for long periods in a peculiarly unstable, "borderline" condition, repeatedly suspected of becoming lepromatous. The third one presented, in the phase here dealt with, as at one time one of the others did to a less extent, lesions of peculiar, atypical morphology and histology that are designated as "relapse tuberculoid". The persistently borderline cases have long been characterized mainly by repeated reactions of more or less mild degree and repeated, at times continuous, eruptions of papular lesions, the earlier large leprids having subsided relatively early in the course of the disease. The papular elements have usually not been discrete, but diffused peripherally, and when acute they have ordinarily yielded numerous to abundant bacilli; yet they have regularly receded spontaneously or yielded promptly to local treatment in the way of papular leprids, while the new ones appeared coincidentally or in later eruptions. Histologically most of them have been indeterminate, neither tuberculoid nor frank lepromatous. Seldom have they shown any attempt to anlarge, but that development has been seen. At one time in Case 1 a few become miniature circinate leprids, of clear-cut tuberculoid. Nor have these cases become lepromatous, though the prognosis remains uncertain. Other lesions of highly atypical, suspicious character appeared during one period in Case 2, and in the third case such lesions were extensive and very striking. With their diffused outlines, abundant bacilli and peculiar histology, they might easily be taken for lepromata; and, with enclosed or adjoining unaffected areas that are know to have been sites of previous major tuberculoid lesions, against which the infiltration is sharply limited, they might seem to have arisen by lepromatous transformation of such earlier lesions; yet they later subsided in the way of leprids. The explanation of the obvious relationship of these "relapse tuberculoid" lesions to the apparently healed, previously tuberculoid areas is a matter of speculation. There is reason to believe that such lesions have at times been taken to be lepromata, thus confusing the picture of transformation of tuberculoid cases if that occurs, which remains to be established satisfactorily...

Hanseníase/classificação , Hanseníase/complicações , Hanseníase/diagnóstico , Hanseníase/patologia
Int. j. lepr ; 8(3): 333-344, July-Sep. 1940. ilus
Artigo em Inglês | SES-SP, HANSEN, SESSP-ILSLACERVO, SES-SP | ID: biblio-1228372


Our records of the case here reported cover the period from 1930 to the present time, during which period the course of the disease has shown startling developments. In one phase there suddenly appeared numerous bullae, followed by ulceration and finally leading to the formation of a mixture of pigmented, achromic and spotted scars. These phenomena, taken together, used to be regarded by some of the old writers as characteristic of the so-called "lazarine leprosy". The original rapidly spreading macular lesions in our case showed a typical tuberculoid picture both clinically and histologically, with numerous bacilli. The occurrence of bullae in the course of the different types of the disease is discussed. Perhaps because it is commonly known that blisters do sometimes occur in the "pure" neural and the lepromatous types, as well as in acute tuberculoid reaction, "lepra lazarina" is not at present looked upon as a separate type or subtype of the disease and is seldom mentioned. However, the article of Pardo-Castella and Caballero (1931), which described a peculiar monosymptomatic form of the sisease, accompanied by blister formation, which those authors called lazarine leprosy, has awakened anew considerable interest in this old term. Pending restudy of the histology of their cases in the light of current knowledge of tuberculoid leprosy, it is suggested the term lazarine leprosy be limited to this special Cuban form. We have tentatively designated our case as one of bullous tuberculoid leprosy.

Hanseníase/classificação , Hanseníase/complicações , Hanseníase/diagnóstico , Hanseníase/fisiopatologia