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1.
Indian J Pediatr ; 72(12): 1043-6, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16388154

RESUMO

OBJECTIVE: This prospective study was carried out to assess the diagnostic value of in situ Polymerase Chain Reaction in leprosy, particularly in enhancing the histopathological diagnosis. METHOD: Clinical examination of 20 patients (< 16 yr) was done and skin smear for AFB was prepared. Biopsy of lesion site was taken for histopathological examination and in situ PCR testing. RESULTS: The histopathological examination confirmed the clinical diagnosis in 45% cases only; non-specific histopathology was reported in the remaining 55% cases. In situ PCR showed a positivity of 57.1% in early/localized form of leprosy (IIBT) and 61.5% in (BB/BL) group. When compared to histopathology examination, a significant enhancement of 15% in diagnosis was seen. With in situ PCR, the diagnosis could be confirmed in 4/11 (36.3%) cases with non-specific histopathological features, (which is common in early disease) in addition to confirmation of 8/9 (88.8%) histopathologically-confirmed tissue sections. Histopathology and in situ PCR, combined together, confirmed the diagnosis in 13/20 cases (65% of total cases). CONCLUSION: Thus, in situ PCR is an important diagnostic tool especially in early and doubtful cases of leprosy.


Assuntos
DNA Bacteriano/isolamento & purificação , Hanseníase/diagnóstico , Mycobacterium leprae/isolamento & purificação , Reação em Cadeia da Polimerase , Adolescente , Criança , Pré-Escolar , Feminino , Humanos , Hanseníase/genética , Hanseníase/patologia , Masculino , Mycobacterium leprae/genética , Estudos Prospectivos , Pele
2.
Artigo em Inglês | MEDLINE | ID: mdl-17656891

RESUMO

A 23-year-old male with multiple lentigenes, pulmonary stenosis, hyperelasticity of skin, hypermobile metacarpophalangeal joints is reported as a forme fruste form of leopard syndrome.

3.
Artigo em Inglês | MEDLINE | ID: mdl-17656947

RESUMO

A-16-year old female with laxity, atrophy, wrinkling and telangiectases of skin of both upper eyelids is being reported. These changes were preceded by recurrent, painless edema of eye lids, starting at the age of 14 years.

4.
Artigo em Inglês | MEDLINE | ID: mdl-17664739

RESUMO

An extensive, non-familial, unilateral nevus comedonicus involving right half of the trunk and both sides of the face is being reported, because of its rarity and unusual presentation.

5.
Artigo em Inglês | MEDLINE | ID: mdl-20944314

RESUMO

Comparative histopathology was studied in 25 cases of scabies versus 25 cases of nodular scabies which were selected from Dermato-Venereology out patients. Salient differences observed were that in scabies lifting of stratum corneum at places was seen in all 100% cases, spongiosis in 100%, spongiotic vesicles in 28%, burrows in 56%, mite in 40% and vasculitis in 28% whereas in nodular scabies acanthosis was seen in 100%, pseudo epitheliomatous hyperplasia in 8%, burrows in 48%, mite in 24% and vasculitis in 84%. In nodular scabies, dermal infiltrate in 32% cases was arranged as lymphoid follicles with admixture of plasma cells and eosinophils.

6.
Artigo em Inglês | MEDLINE | ID: mdl-20947959

RESUMO

One hundred patients with vitiligo were enrolled from out patient department from the year 1990-91 for screening the therapeutic effect of injection placental extract (Placentrex). Patients were divided into two groups of 50 each, age and sex matched and were given two different regimens of treatment. In Regimen- I, patients were given oral psoralens followed by sun exposure after 2 hours and then application of topical betamethasone-17 valerate. In Regimen-ll patients were given treatment as in regimen-l along with injection of placental extract. It was found that initial response rate of Regimen-ll therapy was 36% higher than Regimen-l whereas total efficacy of R-ll was 20% higher than R-l.

7.
Artigo em Inglês | MEDLINE | ID: mdl-20947994

RESUMO

A 65-year-old female had psoriasiform, depigmented, angiolupoid, lupus pernio, papular and verrucous plague type of cutaneous sarcoidosis. Systemic involvement (hepatomegaly, bilateral persistent swelling of the parotid glands, pain in the hands, weakness and atrophic rhinitis of the left nasal cavity) was also present. The histopathological features of a non-caseating epithelioid cell granuloma with giant cells confirmed the diagnosis. The patient is responding favourably to prednisolone 10 mg daily.

8.
Artigo em Inglês | MEDLINE | ID: mdl-20948033

RESUMO

A rare case of nodular colloid degeneration is reported which presented clinically as plaques studded with soft yellow papules simulating adult colloid milia superimposed only on herpes zoster scars of right side of the face.

9.
Artigo em Inglês | MEDLINE | ID: mdl-20948079
10.
Artigo em Inglês | MEDLINE | ID: mdl-20948084
11.
Artigo em Inglês | MEDLINE | ID: mdl-20952881
12.
Hum Immunol ; 32(4): 246-53, 1991 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-1783571

RESUMO

Among major histocompatibility complex class II antigens, HLA-DR2 appears to have a much larger degree of polymorphism than usually recognized by routine serology or restriction fragment length polymorphisms. We have utilized oligonucleotide probes to further identify the DR2 specificity and its molecular subtypes on the basis of specific DNA sequences as they occur in a select sample from the Asian Indian population. In addition, oligonucleotide typing of HLA-DQA1 and -DQB1 genes allowed us to determine specific associations of DRB1, DRB5, DQA1, and DQB1 alleles in DR2 individuals. A set of 60 oligonucleotide probes were hybridized to polymerase chain reaction (PCR)-amplified DNA from DR2 homozygous or heterozygous individuals. The most common DR2 subtypes that occurred in this selected population are: DRB1*1501 (60%), DRB1*1502 (33.8%), and DRB1*1602 (6.2%). No example of DRB1*1601 was detected. By combining these results with the allelic variations at DQA1 and DQB1, we were able to detect at least seven different haplotypes, the most common being DRB1*1502-DRB5*0102-DQA1*0103-DQB1*0601 and DRB1*1501-DRB5*0101-DQA1*0102-DQB1*0502. At least five unexpected combinations, not reported among Western Caucasians, were noticed in this sample. Thus oligonucleotide typing is a valuable tool for defining further polymorphisms in the HLA-D region as exemplified by its applications to typing DR2-positive patients with tuberculoid leprosy and pulmonary tuberculosis.


Assuntos
Antígeno HLA-DR2/genética , Polimorfismo Genético/genética , Alelos , Sequência de Bases , DNA/genética , Antígenos HLA-DQ/genética , Cadeias alfa de HLA-DQ , Cadeias beta de HLA-DQ , Haplótipos , Humanos , Índia , Hanseníase Tuberculoide/genética , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Sondas de Oligonucleotídeos , Reação em Cadeia da Polimerase , Tuberculose Pulmonar/genética
14.
Tissue Antigens ; 21(5): 380-4, 1983 May.
Artigo em Inglês | MEDLINE | ID: mdl-6868057

RESUMO

Investigations for the HLA-A, -B, -C and -DR antigens were conducted on 124 random North Indian patients with confirmed diagnosis of pulmonary tuberculosis by the demonstration of acid fast bacilli in the sputum. 109 appropriately matched controls from the same ethnic background were also tissue typed. No significant deviation was observed in the HLA-A, -B, and -C locus antigens. With the HLA-DR typing, there was a marginal increase in DR2 and a concurrent significant decrease in DRw6 in the patient group. These deviations were, however, insignificant when correction for the P value was made. ABO blood group typing results indicate that blood group 'O' may afford protection against TB. The involvement of both DR2 and DRw6 is interesting as it is also implicated in leprosy, another mycobacterial disease. The results suggest the possibility of a common gene in the MHC for both tuberculosis and leprosy.


Assuntos
Antígenos HLA/genética , Tuberculose Pulmonar/genética , Sistema ABO de Grupos Sanguíneos/genética , Adolescente , Adulto , Idoso , Tipagem e Reações Cruzadas Sanguíneas , Feminino , Frequência do Gene , Humanos , Índia , Masculino , Pessoa de Meia-Idade
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