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Indian J Lepr ; 59(2): 152-7, 1987.
Artigo em Inglês | MEDLINE | ID: mdl-3309085


Cell free extracts of armadillo derived M. leprae, M. phlei, M. smegmatis and normal armadillo liver were analysed for the two key enzymes of TCA cycle. Aconitase activity was assayed in the presence of inhibitor fluorocitrate and it was observed that cell free extracts from cultivable mycobacteria as well as aramadillo derived M. leprae had this enzyme activity and 66-82% of this activity was inhibited by 0.1 mM fluorocitrate. 74% of M. leprae derived enzyme activity was inhibited by fluorocitrate in contrast with armadillo derived enzyme which was only 29% inhibited by fluorocitrate. PAGE separation of cell free extracts and staining for Isocitrate dehydrogenase (ICD) activity showed that an additional bond of ICD activity was demonstrable in the cell free extracts of armadillo derived M. leprae and this was NADP dependent. The mobility (ef) of this band of activity was in the same range as ICD from cultivable mycobacteria and much lower than ICD from normal armadillo liver. From this study and from the previously reported work, it is concluded that like other mycobacteria TCA cycle is operative in M. leprae.

Aconitato Hidratase/metabolismo , Isocitrato Desidrogenase/metabolismo , Mycobacterium leprae/enzimologia , Aconitato Hidratase/antagonistas & inibidores , Animais , Tatus , Citratos/farmacologia , Ciclo do Ácido Cítrico , Eletroforese em Gel de Poliacrilamida , Mycobacterium/enzimologia , Mycobacterium phlei/enzimologia
Biochem J ; 208(2): 377-82, 1982 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-7159406


A procedure for isolation and separation of purified luminal-membrane and basolateral-membrane vesicles from adult and newborn rabbit renal cortex by using Ca2+/Mg2+ precipitation, differential centrifugation and a self-orienting Percoll-gradient centrifugation is described. The purity of the membrane-vesicle suspensions was examined by electron microscopy and by measuring the activity of several marker enzymes. The activity of Na+ + K+-stimulated ATPase in the fraction mainly containing adult rabbit basolateral-membrane vesicles was enriched 16-fold, and the activity of alkaline phosphatase in the fraction mainly containing luminal-membrane vesicles was increased 13-fold, compared with the homogenate. Similar results were obtained with kidneys from newborn rabbits. Uptake studies, with a rapid filtration technique and the spectrophotometric method described in an accompanying paper [Kragh-Hansen, Jørgensen & Sheikh (1982) Biochem. J. 208, 359-368], showed that both adult and newborn rabbit luminal-membrane vesicles, in contrast with the basolateral-membrane preparations, possess an Na+-dependent electrogenic transport system for L-proline. Adult rabbit luminal-membrane vesicles take up citrate and L-malate by Na+-dependent electrogenic processes, whereas adult rabbit basolateral membrane vesicles do not exhibit electrogenic uptake of citrate. By contrast, these vesicles show Na+-dependent electrogenic uptake of L-malate.

Fracionamento Celular/métodos , Córtex Renal/ultraestrutura , Animais , Membrana Celular/enzimologia , Membrana Celular/metabolismo , Membrana Celular/ultraestrutura , Citratos/metabolismo , Ácido Cítrico , Técnicas In Vitro , Córtex Renal/enzimologia , Córtex Renal/metabolismo , Malatos/metabolismo , Microscopia Eletrônica , Prolina/metabolismo , Coelhos
Boll Ist Sieroter Milan ; 56(4): 384-6, 1977 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-334196


The multiplication of Mycobacterium leprae was modified by graded dilutions of organic acids. 0.01%-0.05% gluconic acid inhibited its multiplication. 0.005% of it promoted the growth of 2 out of 6 strains. 0.2% - 1.0% glucuronic acid promoted the multiplication of the majority of strains. 2.0% inhibited their multiplication, and 0.05% promoted the growth of one strain. Galacturonic and pyruvic acids were active in 0.2 - 2.0% concentrations, while the activity of citric acid was mainly noted at 1.0 and 2.0% concentrations.

Mycobacterium leprae/crescimento & desenvolvimento , Diferenciação Celular/efeitos dos fármacos , Citratos/farmacologia , Meios de Cultura , Gluconatos/farmacologia , Glucuronatos/farmacologia , Piruvatos/farmacologia