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1.
Antimicrob Agents Chemother ; 45(3): 800-4, 2001 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11181364

RESUMO

The Mycobacterium bovis P55 gene, located downstream from the gene that encodes the immunogenic lipoprotein P27, has been characterized. The gene was identical to the open reading frame of the Rv1410c gene in the genome of Mycobacterium tuberculosis H37Rv, annotated as a probable drug efflux protein. Genes similar to P55 were present in all species of the M. tuberculosis complex and other mycobacteria such as Mycobacterium leprae and Mycobacterium avium. By Western blotting, P55 was located in the membrane fraction of M. bovis. When transformed into Mycobacterium smegmatis after cloning, P55 conferred aminoglycoside and tetracycline resistance. The levels of resistance to streptomycin and tetracycline conferred by P55 were decreased in the presence of the protonophore carbonyl cyanide m-chlorophenylhydrazone and the pump inhibitors verapamil and reserpine. M. smegmatis cells expressing the plasmid-encoded P55 accumulated less tetracycline than the control cells. We conclude that P55 is a membrane protein implicated in aminoglycoside and tetracycline efflux in mycobacteria.


Assuntos
Proteínas de Bactérias/metabolismo , Proteínas de Transporte/metabolismo , Proteínas de Membrana Transportadoras , Mycobacterium bovis/metabolismo , Mycobacterium tuberculosis/metabolismo , Resistência a Tetraciclina/fisiologia , Motivos de Aminoácidos , Antibacterianos/farmacologia , Proteínas de Bactérias/isolamento & purificação , Proteínas de Transporte/antagonistas & inibidores , Proteínas de Transporte/isolamento & purificação , Bases de Dados Factuais , Resistência a Múltiplos Medicamentos , Lipoproteínas/fisiologia , Testes de Sensibilidade Microbiana , Mycobacterium bovis/efeitos dos fármacos , Mycobacterium tuberculosis/efeitos dos fármacos , Estreptomicina/farmacologia , Frações Subcelulares , Tetraciclinas , Verapamil/farmacologia
2.
Indian J Lepr ; 64(3): 341-7, 1992.
Artigo em Inglês | MEDLINE | ID: mdl-1431323

RESUMO

Skin scrapings from five different active sites were collected from 14 leprosy patients and inoculated into medium V. Skin scrapings from three leprosy patients were inoculated into medium V 1. All the cultures were incubated at 8-10 degrees C. M. tuberculosis H37Rv, pretreatment isolates and streptomycin resistant strains were inoculated into medium V, with and without antibiotics, and incubated at 8-10 degrees C as well as 37 degrees C. Smears were made from the M. leprae and M. tuberculosis cultures at 0 hours and at different time points. The number of bacilli in the smears were counted. There was no increase in the number of M. leprae or M. tuberculosis in any of the cultures.


Assuntos
Mycobacterium leprae/crescimento & desenvolvimento , Mycobacterium tuberculosis/crescimento & desenvolvimento , Meios de Cultura , Resistência Microbiana a Medicamentos , Mycobacterium leprae/efeitos dos fármacos , Mycobacterium tuberculosis/efeitos dos fármacos , Estreptomicina/farmacologia
5.
s.l; s.n; jan. 1982. 6 p. tab, graf.
Não convencional em Inglês | SES-SP, HANSEN, SESSP-ILSLACERVO, SES-SP | ID: biblio-1240470

RESUMO

A method of quantitative estimation to determine the interaction of antituberculosis drugs is suggested. The design of experiments, performed on 7H11 agar plates, is adjusted to the following statistical treatment by combined use of probit analysis and isobologram methods. By plotting the values reflecting the inhibition of 75% of the bacterial population (ED75) with their confidence limits on the isobologram, it was found that the clearest results proving synergism between the drugs could be obtained. Six 2-drug combinations and 6 3-drug combinations were tested against strains of Mycobacterium intracellulare (serovar 8), and a synergistic effect was demonstrated in most of them. These were various combinations of rifampin, streptomycin, ethambutol, and ethionamide. The application of probit analysis to the data derived from testing single drugs can provide a quantitative estimation of the actual drug resistance of the M. intracellulare strains.


Assuntos
Estreptomicina/farmacologia , Etambutol/farmacologia , Etionamida/farmacologia , Micobactérias não Tuberculosas , Mycobacterium , Rifampina/farmacologia , Sinergismo Farmacológico , Testes de Sensibilidade Microbiana
6.
Int J Lepr Other Mycobact Dis ; 48(3): 277-84, 1980 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-7002812

RESUMO

M. lepraemurium grow well in a Balb/c 3T3 recloned cell line (A31). In monolayer culture, the average generation time of M. lepraemurium in A31 cells was 5.3 to 9.4 days at 37 degrees C. A31 cells are very sensitive to infection with M. lepraemurium. Bacterial increases were readily apparent 30 days after inoculating 2 X 10(5) A31 cells in monolayer culture with only six bacilli. The intracellular bacilli were well transferred without apparent losses by host cell transfer. The growth of intracellular bacilli was inhibited by streptomycin 100 micrograms/ml, clindamycin 25 micrograms/ml, INH 5 micrograms/ml, and rifampin 5 micrograms/ml. When streptomycin or clindamycin was removed from the culture medium after 41 days of treatment and the cultivation continued in drug-free medium, the intracellular bacilli began to multiply once more without a lag period. When the intracellular bacilli were treated with INH for 35 days or rifampin for ten days, growth resumed, but only after lag periods after removal of these drugs. We utilized agar suspension techniques for the cultivation of host cells M. lepraemurium because normal cells or transformed cells ceased undergoing cell division and remained healthy for long periods of time in agar medium. M. lepraemurium grew well in A31, A31 transformed by polyoma virus, nude mouse foot pad, chick embryo, and human neuroblastoma cells, utilizing the agar suspension technique. The agar suspension cell culture method should provide useful clues for the cultivation of M. leprae.


Assuntos
Ágar , Técnicas Bacteriológicas , Mycobacterium lepraemurium/crescimento & desenvolvimento , Ágar/farmacologia , Animais , Linhagem Celular , Embrião de Galinha , Clindamicina/farmacologia , Meios de Cultura/farmacologia , Técnicas de Cultura , Humanos , Camundongos , Mycobacterium lepraemurium/efeitos dos fármacos , Rifampina/farmacologia , Estreptomicina/farmacologia
7.
Acta Leprol ; (75): 49-54, 1979.
Artigo em Inglês | MEDLINE | ID: mdl-121020

RESUMO

Once M. leprae is grown on artificial media in the test tube, it might prove to have a great variety of characteristics quite different to those expected from our knowledge of M. leprae isolated from the susceptible host. The cultures might be slow or fast growing, pigmented or colorless, pathogenic for the armadillo, or not; they might produce limited disease in the foot pad of mice, or the contrary. The in vitro M. leprae culture might or might not provoke a lepromine reaction; the culture might grow at a lower or higher temperature. It is well documented that mycobacteria show great differences in elasticity and adaptability to cultivation conditions. It is absolutely certain that once grown in a test tube, M. leprae will behave as an atypical species. However, each individual culture of M. leprae obtained in vitro will have the same drug sensitivity pattern as in the lepromatous leprosy patient from whom it was cultivated.


Assuntos
Mycobacterium leprae/crescimento & desenvolvimento , Adaptação Fisiológica , Meios de Cultura , Resistência Microbiana a Medicamentos , Humanos , Hansenostáticos/farmacologia , Hanseníase/microbiologia , Mycobacterium leprae/efeitos dos fármacos , Mycobacterium leprae/metabolismo , Pigmentos Biológicos/biossíntese , Estreptomicina/farmacologia
10.
Microbios ; 19(76): 117-23, 1977.
Artigo em Inglês | MEDLINE | ID: mdl-366337

RESUMO

Sensitivity to anti-leprous drugs of M. leprae isolated from an L-type leprosy patient was tested using M--Y 14b liquid medium by direct and indirect methods. The results revealed that the strain, SR61-L74, was almost completely resistant to DDS, and responded only to the long-term administration of Streptomycin and Isoniazid. However, the strain was completely sensitive to rifampicin which had never been administered previously. The subsequent administration of rifampicin resulted in a rapid improvement of the patient's clinical symptoms. It can be concluded that the in vitro method, both direct and indirect, to test the sensitivity of M. leprae to anti-leprous drugs is economic, and accordingly available practically as one of the routine examinations in the laboratory of ordinary leprosaria. This must be very beneficial to the treatment of leprosy patients.


Assuntos
Hansenostáticos/farmacologia , Hanseníase/microbiologia , Mycobacterium leprae/efeitos dos fármacos , Dapsona/farmacologia , Resistência Microbiana a Medicamentos , Humanos , Isoniazida/farmacologia , Hanseníase/tratamento farmacológico , Testes de Sensibilidade Microbiana/métodos , Rifampina/farmacologia , Estreptomicina/farmacologia
11.
Int J Lepr Other Mycobact Dis ; 43(3): 234-8, 1975.
Artigo em Inglês | MEDLINE | ID: mdl-176120

RESUMO

A simple radiometric method has been developed for evaluating the effect of drugs on the metabolism of M. lepraemurium. The method is based on the measurement of the 14CO2 produced through bacterial metabolism of acetate-U-14C. Seventeen drugs were tested: bacitracin, cephaloridine, chloramphenicol, cycloserine, dactinomycin, DDS, ethionamide, INH, kanamycin, methenamine mandelate, nitrofurantoin, oxacillin, polymyxin B, rifampicin, streptomycin, sulfadimethoxine and vancomycin. The drugs which caused most marked inhibition were chloramphenicol, INH, ethionamide and nitrofurantoin in order of increasing effectiveness. The radiometric study which is completed in 15 days permits direct study of the drug effect on the metabolism of M. lepraemurium and a more rapid screening of antileprosy drugs than has previously been possible. Currently, these observations are being extended to studies of the structure-activity relationships of antileprosy drugs and the metabolism and drug susceptibility of M. leprae in vitro.


Assuntos
Antibacterianos/farmacologia , Infecções por Mycobacterium/tratamento farmacológico , Mycobacterium lepraemurium/efeitos dos fármacos , Bacitracina/farmacologia , Cefaloridina/farmacologia , Cloranfenicol/farmacologia , Dactinomicina/farmacologia , Dapsona/farmacologia , Etionamida/farmacologia , Isoniazida/farmacologia , Canamicina/farmacologia , Metenamina/farmacologia , Testes de Sensibilidade Microbiana , Nitrofurantoína/farmacologia , Oxacilina/farmacologia , Polimixinas/farmacologia , Radiometria , Rifampina/farmacologia , Estreptomicina/farmacologia , Sulfadimetoxina/farmacologia , Vancomicina/farmacologia
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