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Infect Immun ; 41(1): 121-7, 1983 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-6345387


Macrophage cultures pulsed with viable Mycobacterium leprae were assessed for erythrocyte rosetting in three groups of individuals, i.e., normal subjects, and tuberculoid and lepromatous patients. Of these, only the lepromatous group showed a reduction in rosetting ability after infection with M. leprae. The specificity of such a reduction pattern was confirmed by using various mycobacteria to infect the macrophages. A threshold effect was noted in all three groups. Although a reduction was obtained in the amount of rosetting of macrophages from lepromatous patients with 10(4) acid-fast bacilli per culture, tuberculoid and normal macrophages resisted such an effect with as large a dose as 20 X 10(6) to 30 X 10(6) and 30 X 10(6) bacilli per culture, respectively. The M. leprae-caused alterations in macrophages from lepromatous patients were reversible by treatment with trypsin and colchicine. Cytochalasin B and Tween 80 were unable to alter the pattern. Treatment of cells with neuraminidase was inconclusive since it enhanced rosetting values of both control and infected cultures. These manipulations were significant in elucidating the target point of the host (macrophage) and parasite (M. leprae) interaction and in delineation of the external and internal effects upon the macrophages. Both M. leprae and macrophages were participants in Fc reduction, as treatment of the former with rifampicin and of the latter with cyclocheximide significantly augmented the rosetting ability. In conclusion, it appears that M. leprae, upon entering a lepromatous macrophage, initiates the production of a protein which acts via the microtubules to alter membrane topography. It is possible that the altered membrane prevents effective macrophage-lymphocyte interaction. This could be one of the mechanisms by which cell-mediated immunity is suppressed in lepromatous leprosy.

Hanseníase/imunologia , Macrófagos/imunologia , Mycobacterium leprae/fisiologia , Animais , Células Cultivadas , Colchicina/farmacologia , Cicloeximida/farmacologia , Citocalasina B/farmacologia , Humanos , Macrófagos/microbiologia , Neuraminidase/farmacologia , Polissorbatos/farmacologia , Receptores Fc/fisiologia , Rifampina/farmacologia , Formação de Roseta , Tripsina/farmacologia
Arch Dermatol ; 111(3): 331-4, 1975 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-164154


Immunoglobulin deposits were detected in ten of 13 biopsy specimens from apparently uninvolved skin of patients with lepromatous leprosy. There were deposits of IgM at the dermoepidermal junction in the skin of five patients, and deposits of IgM along the dermal collagen and elastic fibers in the skin of the other five. The deposits were eluted with acid buffers and high molarity salt solution. Circulating IgG antibodies to intercellular substance of epithelial cells, similar to those present in pemphigus vulgaris, were found in 25% of patients with lepromatous leprosy who were studied. These antibodies appeared to be different from the skin-bound immunoglobulin deposits.

Anticorpos Anti-Idiotípicos , Imunoglobulinas , Hanseníase/imunologia , Pele/imunologia , Adulto , Animais , Anticorpos , Colágeno/imunologia , Desoxirribonucleases/farmacologia , Células Epiteliais , Epitélio/imunologia , Feminino , Imunofluorescência , Cobaias/imunologia , Humanos , Imunoglobulina G , Hanseníase/enzimologia , Hanseníase/patologia , Masculino , Colagenase Microbiana/farmacologia , Pessoa de Meia-Idade , Neuraminidase/farmacologia , Ácido Periódico/farmacologia , Coelhos/imunologia , Pele/efeitos dos fármacos , Pele/patologia