Cross-reactivity of anti-10kD heat shock protein antibodies in leprosy and tuberculosis patients.
Medicina (B Aires)
; 57(5): 581-6, 1997.
Article
in En
| MEDLINE
| ID: mdl-9674227
ABSTRACT
The response to recombinant 10-kD heat shock protein (HSP) of Mycobacterium leprae (rML10) was evaluated by indirect ELISA in sera from leprosy patients, household contacts, tuberculosis patients and healthy controls in a leprosy-endemic area in the North East of Argentina. Some technical parameters were analyzed within-assay and between-assay variability, dose-response curves and detectability indexes (specificity and sensitivity) of ELISA applied to measure anti-10 kDa antibodies. High levels of these antibodies have already been reported in positive bacilloscopy patients; herein we have also demonstrated that tuberculosis patients sera cross-react with this M. leprae antigen. This test seems to have a low sensitivity and specificity for leprosy detection; it confirms that antibodies against highly conserved HSP antigens are important in the polyclonal response against mycobacterial epitopes in leprosy as well as in tuberculosis.
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Theme:
Complicacoes
/
Geral
/
Prevencao_controle
/
Tratamento_medicamentoso
Database:
MEDLINE
Main subject:
Tuberculosis
/
Chaperonin 10
/
Leprosy
/
Antibodies, Bacterial
/
Mycobacterium leprae
/
Antigens, Bacterial
Type of study:
Diagnostic_studies
Language:
En
Journal:
Medicina (B Aires)
Year:
1997
Document type:
Article