ABSTRACT
OBJECTIVE: To study the anticoagulant activity of different portions of leech ethanol extracts (LEEs). METHODS: Anticoagulant activity was determined by measuring prothrombin time(PT), thrombin time (TT), activated partial throboplstin time (APTT) and fibrinogen coagulation time (FCT). RESULTS: PT, TT, APTT and FCT were remarkably prolonged by ethyl acetate portion of LEEs. Portions of petroleum ethrer, n-butanol and water extracted from LEEs was much weaker on anticoagulant activity. CONCLUSION: The anticoagulant effect of ethyl acetate extract portion of LEEs is the strongest among the four portions, and this results may be from its inhibitory effect on thrombin-catalyzed fibrinogen hydrolysis.
Subject(s)
Anticoagulants/pharmacology , Blood Coagulation/drug effects , Leeches/chemistry , Materia Medica/pharmacology , Animals , Anticoagulants/isolation & purification , Blood Coagulation Tests , Ethanol , Humans , Materia Medica/isolation & purification , Partial Thromboplastin Time , Prothrombin Time , Thrombin TimeABSTRACT
A polypeptide, SV-PP-2, was purified from Agkistrodon blomhoffii Bioe by HPLC C18 column. The SV-PP-2 has molecular weight of 23339.00 Da, and can inhibit the ADP-induced platelet aggregation in dose-dependent manner. There have been no such polypeptides reported yet which has inhibition of platelet aggregation and similar molecular weight to SV-PP-2.
Subject(s)
Agkistrodon , Materia Medica/pharmacology , Peptides/isolation & purification , Peptides/pharmacology , Platelet Aggregation Inhibitors/pharmacology , Snake Venoms/chemistry , Animals , Chromatography, High Pressure Liquid/methods , Dose-Response Relationship, Drug , Female , In Vitro Techniques , Male , Materia Medica/isolation & purification , Platelet Aggregation/drug effects , Platelet Aggregation Inhibitors/isolation & purification , RabbitsABSTRACT
This is the first report to purify a new peptide SV-PP-1 from Agkistrodon blomhoffii Bioe. SV-PP-1 has molecular weight of 1234.616 Da and can inhibit on platelet aggregation. SV-PP-1 significantly inhibited the ADP-induced platelet aggregation. It has been found up to now that SV-PP-1 is the smallest peptide inhibiting on platelet aggregation from snake venom.