ABSTRACT
Despite optimised diagnosis and treatment, prostate cancer can only be cured in a specific subset of patients. Advanced prostate cancer may lead to complications that severely impair the patient's quality of life, e. g. recurrent intravesical blood clotting due to local tumor necrosis. We report the successful use of the homeopathic remedy Thlaspi bursa pastoris in 2 patients for whom conventional treatment was not sufficiently effective. These case reports imply that complementary or alternative medical treatment may be an efficient adjunctive treatment in patients with advanced prostate cancer.
Subject(s)
Bone Neoplasms/secondary , Bone Neoplasms/therapy , Complementary Therapies , Materia Medica/therapeutic use , Phytotherapy , Prostatic Neoplasms/pathology , Prostatic Neoplasms/therapy , Spinal Neoplasms/secondary , Spinal Neoplasms/therapy , Thlaspi , Aged , Bone Neoplasms/pathology , Combined Modality Therapy , Humans , Male , Middle Aged , Neoplasm Staging , Spinal Neoplasms/pathologyABSTRACT
OBJECTIVE: The activity of deer serum albumin on proliferation of rat osteogenic-like cells UMR-106 and the IGF-I secretion were investigated in order to elucidate pilose antler's bone-strengthening mechanism. METHOD: Deer serum albumin was isolated from freeze-dry pilose antler powder extract. The methods were Sephacryl S-200HR gel filtration, POROS 20QE ion-exchange and TSK G3000SW chromatographies. The effect of deer serum albumin on proliferatio of UMR-106 cells was assaied by MTT, and the secretion of IGF-I of UMR-106 cells was assaied by RIA. RESULT: Deer serum albumin, with the molecular weight of 56.3 kDa, significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF-I secretion. When concentration of deer serum albumin reached 0.149 microg x mL(-1), UMR-106 cell proliferation rate was 241.03% and IGF-I secretion was 66.89 ng x mL(-1). CONCLUSION: The concentration of deer serum albumin, from 14.9 ng x mL(-1) to 14.90 microg x mL(-1), significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF- I secretion.
Subject(s)
Antlers , Cell Proliferation/drug effects , Materia Medica/pharmacology , Osteosarcoma/pathology , Serum Albumin/pharmacology , Animals , Antlers/chemistry , Bone Neoplasms/pathology , Cell Line, Tumor , Deer , Insulin-Like Growth Factor I/metabolism , Materia Medica/isolation & purification , Osteoblasts/metabolism , Osteoblasts/pathology , Rats , Serum Albumin/isolation & purificationABSTRACT
OBJECTIVE: To investigate the modulation of pilose antler extract (PAE) on rat osteogenic cells UMR-106 in vitro. METHOD: Component P2 of PAE was isolated by Sephacryl S-200HR gel filtration chromatography. The proliferative effects of P2 and other components isolated by Sephacryl S-200HR on UMR-106 cells were investigated by MTT assay. RESULT: The P2 could significantly increase the proliferation rate of osteogenic cells. When the protein concentration of P2 was between 0.972 mg x L(-1) and 97.2 mg x L(-1), it could inhibit the proliferation of UMR-106 cells. But while the concentration was equal to or greater than 97.2 mg x L(-1), the P2 could increase the proliferation rate of cells, especially 477.92% at 9.72 g x L(-1), which was approximated to 499.62% of PAE. The molecular weight of the P2 was about 59 kDa determined by SDS-PAGE. On the other hand, inhibition was also observed in the sample of the P3, P4 and P5. CONCLUSION: Those regulative factors in PAE which have different molecular weight can affect the proliferation of UMR-106 cells two-wayly. And this adjustment also relies on the dose of those factors. This finding may help us to understand the possible mechanism of Chinese traditional medicine from animal materials.